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[187299_at]

Description F48E8.6 /REP_DB=WormBase Gene ID /WP=CE02758 /TR=SW:Q09568 /GB=AAC46543.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 96      4grp_highest_8_8 > 26      4grp_high_8_8 > 58      6grp_highest_nonorm > 72      6grp_total > 85      4grp_total > 61      6grp_highest_sub > In      6grp_high1_sub > In      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 230
1 2 3 4 range
A 540.6
(3)
P 		391.6
(-3)
P 		516
(2)
P 		401.6
(-2.6)
P 		149
B 482.4
(1.9)
P 		474.8
(1.6)
P 		516.6
(3)
P 		335.8
(-3)
P 		181
C 513.4
(2.5)
P 		452.1
(0.8)
P 		532
(3)
P 		310.9
(-3)
P 		221
avr 512.1
(2.7) 		439.5
(0.1) 		521.5
(3) 		349.4
(-3) 		172

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 313
1 2 3 4 5 6 range
A 326.1
(-2.5)
P 		394.9
(-1)
P 		301.1
(-3)
P 		482.4
(0.9)
P 		478.7
(0.8)
P 		582
(3)
P 		281
B 383.9
(0.6)
P 		314.3
(-2.2)
P 		293.5
(-3)
P 		436.6
(2.7)
P 		393.4
(1)
P 		443.6
(3)
P 		150
C 516.2
(2.1)
P 		268.7
(-3)
P 		559.2
(3)
P 		426.3
(0.3)
P 		465
(1.1)
P 		474.7
(1.3)
P 		291
avr 408.7
(-0.1) 		326
(-3) 		384.6
(-1) 		448.4
(1.2) 		445.7
(1.1) 		500.1
(3) 		174
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 144
1 2 3 4 range
A 196.8
(3)
P 		85
(-1.9)
P 		134.9
(0.3)
P 		58.8
(-3)
P 		138
B 153.8
(3)
P 		117.3
(0.3)
P 		119.7
(0.5)
P 		71.6
(-3)
P 		82
C 170
(2.3)
P 		130
(0.5)
P 		185.4
(3)
P 		52.4
(-3)
P 		133
avr 173.5
(3) 		110.8
(-0.3) 		146.7
(1.6) 		60.9
(-3) 		113

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 131
1 2 3 4 5 6 range
A 60.1
(-3)
P 		140.4
(2.7)
P 		65.6
(-2.6)
P 		122.3
(1.4)
P 		144.5
(3)
P 		141.7
(2.8)
P 		84
B 133.6
(1)
P 		104.6
(-0.9)
P 		71.3
(-3)
P 		115.6
(-0.2)
P 		159.8
(2.6)
P 		165.4
(3)
P 		94
C 191.3
(3)
P 		82
(-3)
P 		142.3
(0.3)
P 		85.7
(-2.8)
P 		150.5
(0.8)
P 		158.9
(1.2)
P 		109
avr 128.3
(0.4) 		109
(-1.5) 		93.1
(-3) 		107.9
(-1.6) 		151.6
(2.6) 		155.3
(3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187299_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7180
Sequence Type Exemplar sequence
Representative Public ID F48E8.6 NCBI
Target Description F48E8.6 /REP_DB=WormBase Gene ID /WP=CE02758 /TR=SW:Q09568 /GB=AAC46543.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065759(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065759 NCBI Caenorhabditis elegans F48E8.6 (F48E8.6) mRNA, complete cds. 11/11 None
GENEFINDER00000003647 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5443262:5446248:1 11/11 None
SNAP00000003637 ENSEMBL cdna:SNAP chromosome:CEL140:III:5443273:5446248:1 11/11 None
F48E8.6 ENSEMBL cdna:known chromosome:CEL140:III:5443262:5446451:1 gene:F48E8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5443260-5446247(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F48E8.6
Entrez Gene 175748 Entrez gene
SwissProt Q09568 EMBL-EBI
WormBase CE02758 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:259704_AT ribonuclease II family protein at
CANINE_2:CFAAFFX.17430.1.S1_S_AT similar to CG16940-PA, isoform A cfa
CHICKEN:GGAAFFX.4840.1.S1_AT similar to hypothetical protein MGC42174 gga
CHICKEN:GGAAFFX.4840.2.S1_S_AT similar to hypothetical protein MGC42174 gga
MG-U74CV2:135825_AT RIKEN cDNA 4930429A22 gene mm
MG-U74BV2:163161_AT RIKEN cDNA 4930429A22 gene mm
MG-U74CV2:170067_I_AT RIKEN cDNA 4930429A22 gene mm
MOE430B:1434090_AT RIKEN cDNA 4930429A22 gene mm
MOUSE430_2:1434090_AT RIKEN cDNA 4930429A22 gene mm
MOE430B:1437951_AT RIKEN cDNA 4930429A22 gene mm
MOUSE430_2:1437951_AT RIKEN cDNA 4930429A22 gene mm
MOE430B:1459328_AT RIKEN cDNA 4930429A22 gene (4930429A22Rik), mRNA mm
MOUSE430_2:1459328_AT RIKEN cDNA 4930429A22 gene (4930429A22Rik), mRNA mm
MOUSE430_2:1459365_AT RIKEN cDNA 4930429A22 gene (4930429A22Rik), mRNA mm
MOE430B:1459365_AT RIKEN cDNA 4930429A22 gene (4930429A22Rik), mRNA mm
MU19KSUBA:TC14614_F_AT RIKEN cDNA 4930429A22 gene (4930429A22Rik), mRNA mm
MU19KSUBA:TC14618_AT RIKEN cDNA 4930429A22 gene (4930429A22Rik), mRNA mm
RG-U34A:RC_AA875511_AT similar to RIKEN cDNA 4930429A22 rn
RAE230A:1372378_AT similar to RIKEN cDNA 4930429A22 rn
RAT230_2:1372378_AT similar to RIKEN cDNA 4930429A22 rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
4540 ribonuclease activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46543 Hypothetical protein F48E8.6 [Caenorhabditis elegans] ref|NP_498160.1| F48E8.6 [Caenorhabditis elegans] sp|Q09568|YR86_CAEEL Hypothetical protein F48E8.6 in chromosome III 0.0
blast CAE61341 Hypothetical protein CBG05180 [Caenorhabditis briggsae] 0.0
blast XP_694466 PREDICTED: similar to RIKEN cDNA 4930429A22 [Danio rerio] 1.0E-167
Protein Domains
Database ID Description E-Value
Pfam IPR001900 EMBL-EBI Ribonuclease II 1.0E-126
Pfam IPR001900 EMBL-EBI Ribonuclease II 1.0E-126
Pfam IPR001900 EMBL-EBI Ribonuclease II 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187299_AT
tgcactttcgagatgcctctttcattttatcatcattttgctctcaacgtggatcattat
acacatttcacatcgccaattcgtcggtatccagatgtgattgttcatcgacaacttgct
gctgctcttggttataatgaaagatctgaaagagttcctgaagagatacaggagatttgc
acccgctgcaatgacacaaagctcgcatcgaaggaagcttcggatgagtctgcgatgctc
tattttggagttttcatccatcaaaccggtccgatgaagtgccaagctgttgttcttggt
gtaatggatctctcattcgacgtcctcatcgttgagtatggagttgtgaagagagtttat
gtggataaaatgaagcgagacttcaacaaatcgaccgagaagcttaccatttactggcca
gcagatccgaatgcggaatccggaaaccgtgaagagttttcgagctcaattcaaatgtgc
aacgtggtttacgtgattctcgtgccgtacaaaagcattgaagtcagcgcgacaattgtt
cgtccgagtctcgagcaacgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCACTTTCGAGATGCCTCTTTCAT 519 577 1846 Antisense
TTCACATCGCCAATTCGTCGGTATC 375 687 1912 Antisense
TGTTCATCGACAACTTGCTGCTGCT 172 563 1947 Antisense
GATACAGGAGATTTGCACCCGCTGC 146 423 2010 Antisense
GCTTCGGATGAGTCTGCGATGCTCT 606 305 2062 Antisense
GAGTTTTCATCCATCAAACCGGTCC 89 397 2093 Antisense
AACCGGTCCGATGAAGTGCCAAGCT 12 147 2109 Antisense
GAAGCTTACCATTTACTGGCCAGCA 282 341 2244 Antisense
GGTTTACGTGATTCTCGTGCCGTAC 495 505 2331 Antisense
GTCAGCGCGACAATTGTTCGTCCGA 46 467 2368 Antisense
GTTCGTCCGAGTCTCGAGCAACGTA 519 293 2383 Antisense
Sequence Source Affymetrix Proprietary Database