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[187294_at]

Description C28H8.1 /REP_DB=WormBase Gene ID /WP=CE01820 /TR=SW:Q09242 /GB=AAA62289.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 77      4grp_highest_8_8 > 0      4grp_high_8_8 > 36      6grp_highest_nonorm > 84      6grp_total > 67      4grp_total > 43      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 135
1 2 3 4 range
A 421.3
(0.3)
P 		458.1
(3)
P 		389.8
(-1.9)
P 		375.3
(-3)
P 		83
B 428.9
(-3)
P 		507.6
(3)
P 		474.9
(0.5)
P 		436.8
(-2.4)
P 		79
C 474.7
(-0.5)
P 		510.3
(3)
P 		448.5
(-3)
P 		450.9
(-2.8)
P 		62
avr 441.6
(-1.3) 		492
(3) 		437.7
(-1.6) 		421
(-3) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 593
1 2 3 4 5 6 range
A 560.8
(-1.2)
P 		510.4
(-2.8)
P 		699.4
(3)
P 		502.4
(-3)
P 		507.8
(-2.8)
P 		654.7
(1.6)
P 		197
B 624.7
(-1.8)
P 		566.9
(-2.8)
P 		664.6
(-1)
P 		558.1
(-3)
P 		674.1
(-0.8)
P 		880.2
(3)
P 		322
C 553.1
(-1.5)
P 		519.5
(-1.8)
P 		435.3
(-2.7)
P 		405.6
(-3)
P 		627.7
(-0.8)
P 		999
(3)
P 		593
avr 579.5
(-1.5) 		532.3
(-2.3) 		599.8
(-1.1) 		488.7
(-3) 		603.2
(-1.1) 		844.6
(3) 		356
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 153.4
(3)
P 		99.4
(-0.3)
P 		101.9
(-0.1)
P 		54.9
(-3)
P 		99
B 136.8
(3)
P 		125.4
(1.4)
P 		110
(-0.7)
P 		93.1
(-3)
P 		44
C 157.1
(3)
P 		146.7
(2.2)
P 		156.3
(2.9)
P 		76
(-3)
P 		81
avr 149.1
(3) 		123.8
(1) 		122.7
(0.9) 		74.7
(-3) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 253
1 2 3 4 5 6 range
A 103.4
(-3)
P 		181.5
(3)
P 		152.3
(0.8)
P 		127.4
(-1.2)
P 		153.2
(0.8)
P 		159.4
(1.3)
P 		78
B 217.4
(-0.7)
P 		188.7
(-1.6)
P 		161.4
(-2.5)
P 		147.8
(-3)
P 		273.9
(1.2)
P 		328.2
(3)
P 		180
C 205
(-0.1)
P 		158.5
(-1.2)
P 		110.8
(-2.3)
P 		81.6
(-3)
P 		203.1
(-0.1)
P 		334.4
(3)
P 		253
avr 175.3
(-0.8) 		176.2
(-0.8) 		141.5
(-2.1) 		118.9
(-3) 		210.1
(0.5) 		274
(3) 		155
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187294_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6010
Sequence Type Exemplar sequence
Representative Public ID C28H8.1 NCBI
Target Description C28H8.1 /REP_DB=WormBase Gene ID /WP=CE01820 /TR=SW:Q09242 /GB=AAA62289.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065886(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065886 NCBI Caenorhabditis elegans C28H8.1 (C28H8.1) mRNA, complete cds. 11/11 None
SNAP00000005439 ENSEMBL cdna:SNAP chromosome:CEL140:III:5926035:5926744:1 11/11 None
GENEFINDER00000005451 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5926035:5926744:1 11/11 None
C28H8.1 ENSEMBL cdna:known chromosome:CEL140:III:5926035:5926933:1 gene:C28H8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5926033-5926743(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C28H8.1
Entrez Gene 175838 Entrez gene
SwissProt Q09242 EMBL-EBI
WormBase CE01820 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498287 C28H8.1 [Caenorhabditis elegans] gb|AAA62289.1| Hypothetical protein C28H8.1 [Caenorhabditis elegans] sp|Q09242|YP91_CAEEL Hypothetical protein C28H8.1 in chromosome III 8.0E-80
blast CAE64362 Hypothetical protein CBG09049 [Caenorhabditis briggsae] 2.0E-70
blast NP_997725 B-cell CLL/lymphoma 7A [Danio rerio] gb|AAQ94590.1| B-cell CLL/lymphoma 7A [Danio rerio] 6.0E-16
Protein Domains
Database ID Description E-Value
Pfam IPR006804 EMBL-EBI BCL7, N-terminal 8.0E-38
Sequence
Target Sequence 
>C. ELEGANS:187294_AT
aaatagatctcatcgcgctgaaacaagaaatcgctcaaaagatgaactccgaaaggttat
caactcattggaaaaagttcgtcggtgggaaaagaagttagttcttatcaaggacacaaa
tattcggatctacaaatgggttccggtgagcgctcaaaacataatggctcctccaaagat
caaggaagtcaaagaagtggatgaggagagcaatcaagtcccatctgctgagaattccca
ggattccaccagcgttacccaaccaccaccacaattcgatatcaacgaagattccaattt
ttccactggcgatcactttgatagtgactccaatcaaactttcgagccacaaaactatca
aggtggagccactggttcaacggatttcagttcgatgcgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATAGATCTCATCGCGCTGAAACA 615 115 24 Antisense
GAACTCCGAAAGGTTATCAACTCAT 565 347 67 Antisense
ACAAATGGGTTCCGGTGAGCGCTCA 178 111 155 Antisense
GCGCTCAAAACATAATGGCTCCTCC 18 289 173 Antisense
GAGGAGAGCAATCAAGTCCCATCTG 38 405 226 Antisense
CAAGTCCCATCTGCTGAGAATTCCC 398 183 238 Antisense
CTGAGAATTCCCAGGATTCCACCAG 679 235 251 Antisense
TTTTCCACTGGCGATCACTTTGATA 547 677 322 Antisense
GACTCCAATCAAACTTTCGAGCCAC 381 375 349 Antisense
AGGTGGAGCCACTGGTTCAACGGAT 602 57 384 Antisense
TTCAACGGATTTCAGTTCGATGCGC 710 653 399 Antisense
Sequence Source Affymetrix Proprietary Database