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[187280_s_at]

Description Y110A7A.18 /REP_DB=WormBase Gene ID /WP=CE23239 /TR=Q9N585 /GB=AAF60414.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 98      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_total > 96      4grp_total > 40      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 454
1 2 3 4 range
A 180.9
(-3)
P 		387.1
(0.8)
P 		504
(3)
P 		410.5
(1.3)
P 		323
B 275.7
(-3)
P 		481
(0.4)
P 		635.2
(3)
P 		549.7
(1.6)
P 		360
C 236.8
(-3)
P 		418.1
(-0.2)
P 		620.1
(3)
P 		377.1
(-0.8)
P 		383
avr 231.1
(-3) 		428.7
(0.3) 		586.4
(3) 		445.8
(0.6) 		355

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1899
1 2 3 4 5 6 range
A 172.3
(-3)
A 		337.9
(-2.5)
P 		376.3
(-2.4)
P 		855.1
(-0.8)
P 		1297.1
(0.6)
P 		2067.7
(3)
P 		1895
B 169.1
(-3)
P 		389.3
(-2.1)
P 		487.4
(-1.7)
P 		883.4
(0)
P 		1132.5
(1.1)
P 		1591.3
(3)
P 		1422
C 221.3
(-3)
P 		348.5
(-2.5)
P 		672.6
(-1.2)
P 		763.2
(-0.8)
P 		1695.4
(3)
P 		1537.3
(2.4)
P 		1474
avr 187.6
(-3) 		358.6
(-2.3) 		512.1
(-1.7) 		833.9
(-0.5) 		1375
(1.6) 		1732.1
(3) 		1545
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 65.9
(-2.5)
P 		84
(-1)
P 		131.8
(3)
P 		60.1
(-3)
P 		72
B 87.9
(-3)
P 		118.8
(0.1)
P 		147.2
(3)
P 		117.1
(-0)
P 		59
C 78.4
(-2.4)
P 		120.2
(-0.8)
P 		216.1
(3)
P 		63.6
(-3)
P 		153
avr 77.4
(-3) 		107.7
(-0.9) 		165
(3) 		80.3
(-2.8) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 562
1 2 3 4 5 6 range
A 31.8
(-3)
A 		120.2
(-1.9)
P 		82
(-2.4)
P 		216.9
(-0.6)
P 		391.4
(1.6)
P 		503.4
(3)
P 		472
B 58.8
(-3)
P 		129.6
(-2.2)
P 		118.4
(-2.3)
P 		234
(-1)
P 		460.1
(1.5)
P 		593.4
(3)
P 		535
C 82
(-3)
P 		106.3
(-2.7)
P 		171.2
(-1.9)
P 		153.5
(-2.1)
P 		548.6
(3)
P 		514.6
(2.6)
P 		467
avr 57.5
(-3) 		118.7
(-2.2) 		123.9
(-2.2) 		201.5
(-1.2) 		466.7
(2.1) 		537.1
(3) 		480
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187280_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.725
Sequence Type Exemplar sequence
Representative Public ID Y110A7A.18 NCBI
Target Description Y110A7A.18 /REP_DB=WormBase Gene ID /WP=CE23239 /TR=Q9N585 /GB=AAF60414.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059134(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059134 NCBI Caenorhabditis elegans PAZ/PIWI domain-containing family member (ppw-2) (ppw-2) mRNA, complete cds. 11/11 None
SNAP00000029218 ENSEMBL cdna:SNAP chromosome:CEL140:I:5158818:5162207:-1 11/11 None
GENEFINDER00000029236 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5158818:5162207:-1 11/11 None
Y110A7A.18 ENSEMBL cdna:known chromosome:CEL140:I:5158530:5162207:-1 gene:Y110A7A.18 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5158817-5162207(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ppw-2
Ensembl Y110A7A.18
Entrez Gene 172152 Entrez gene
SwissProt Q9N585 EMBL-EBI
WormBase CE23239 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60414 Paz/piwi domain-containing protein 2 [Caenorhabditis elegans] ref|NP_491535.1| PAZ/PIWI domain-containing family member (ppw-2) [Caenorhabditis elegans] 0.0
blast CAB04524 Hypothetical protein F58G1.1 [Caenorhabditis elegans] ref|NP_496751.1| F58G1.1 [Caenorhabditis elegans] 0.0
blast T18974 hypothetical protein C06A1.4 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003100 EMBL-EBI Argonaute and Dicer protein, PAZ 4.5E-37
Pfam IPR003165 EMBL-EBI Stem cell self-renewal protein Piwi 2.7E-109
Sequence
Target Sequence 
>C. ELEGANS:187280_S_AT
gaaatcacggaccaatcatctcctatgaagttcctcttgctcgtgttgccatgagaaact
tttcgccggacactcaattgctttatatcgtggtctcgaaggagcacacctatagattct
tcaagaaagagtctggtggatcttccagcggtggatctaacagcgctggcgcaagcaatt
ctggcacattgacttcggcaccgccaaaaccatgggagctcaatattggtccaggactta
ccgttgattatggtgttaccaacccagcatgcaagcagttcttcttgaactcgcacatga
ctcttcagggatcggcaaagaccccgctctatactgttcttgctgatgatcgtaatattg
gtatgtcggcattggaagaattcaccttcaatctgtgtcacctccatcaaattgtcgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATCACGGACCAATCATCTCCTA 127 363 2324 Antisense
TCATCTCCTATGAAGTTCCTCTTGC 492 621 2339 Antisense
AAACTTTTCGCCGGACACTCAATTG 35 127 2379 Antisense
GGAGCACACCTATAGATTCTTCAAG 690 519 2424 Antisense
GCTGGCGCAAGCAATTCTGGCACAT 36 295 2488 Antisense
GCACCGCCAAAACCATGGGAGCTCA 529 301 2521 Antisense
TATGGTGTTACCAACCCAGCATGCA 4 657 2572 Antisense
AACTCGCACATGACTCTTCAGGGAT 645 141 2611 Antisense
CGCTCTATACTGTTCTTGCTGATGA 462 253 2648 Antisense
GAATTCACCTTCAATCTGTGTCACC 315 325 2701 Antisense
GTGTCACCTCCATCAAATTGTCGGA 276 487 2718 Antisense
Sequence Source Affymetrix Proprietary Database