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[187277_s_at]

Description F25B5.2 /REP_DB=WormBase Gene ID /WP=CE01919 /TR=SW:Q09314 /GB=AAC46524.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 50      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 142
1 2 3 4 range
A 7.8
(-3)
A 		24.4
(-1.5)
A 		75.4
(3)
A 		32.5
(-0.8)
A 		68
B 75.6
(-2.7)
A 		149.6
(3)
A 		122.3
(0.9)
A 		71.3
(-3)
A 		78
C 94.5
(-0.1)
A 		146.4
(3)
A 		90.1
(-0.3)
A 		44.7
(-3)
A 		102
avr 59.3
(-2) 		106.8
(3) 		95.9
(1.9) 		49.5
(-3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 673
1 2 3 4 5 6 range
A 91.6
(-2.5)
A 		36
(-3)
A 		112.8
(-2.3)
A 		290.8
(-0.6)
A 		319.9
(-0.3)
P 		666.8
(3)
P 		631
B 122
(-2.4)
A 		63.1
(-3)
A 		130
(-2.3)
A 		106.3
(-2.6)
A 		270.8
(-1)
P 		672.3
(3)
P 		609
C 105.6
(-2.7)
A 		69.1
(-3)
A 		153.7
(-2.2)
A 		201.7
(-1.8)
A 		355.3
(-0.3)
P 		708.6
(3)
P 		640
avr 106.4
(-2.5) 		56.1
(-3) 		132.2
(-2.3) 		199.6
(-1.6) 		315.3
(-0.5) 		682.6
(3) 		627
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 2.9
(-3)
A 		5.3
(-2.1)
A 		19.7
(3)
A 		4.8
(-2.3)
A 		17
B 24.1
(-0.5)
A 		36.9
(3)
A 		28.3
(0.6)
A 		15.2
(-3)
A 		22
C 31.3
(1.1)
A 		42.1
(3)
A 		31.4
(1.1)
A 		7.5
(-3)
A 		35
avr 19.4
(0.2) 		28.1
(3) 		26.5
(2.5) 		9.2
(-3) 		19

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 238
1 2 3 4 5 6 range
A 16.9
(-2.8)
A 		12.8
(-3)
A 		24.6
(-2.5)
A 		73.7
(-0.6)
A 		96.5
(0.4)
P 		162.4
(3)
P 		150
B 42.4
(-2.4)
A 		21
(-3)
A 		31.6
(-2.7)
A 		28.2
(-2.8)
A 		110
(-0.7)
P 		250.7
(3)
P 		230
C 39.1
(-2.5)
A 		21.1
(-3)
A 		39.1
(-2.5)
A 		40.6
(-2.5)
A 		115
(-0.4)
P 		237.2
(3)
P 		216
avr 32.8
(-2.6) 		18.3
(-3) 		31.8
(-2.6) 		47.5
(-2.1) 		107.2
(-0.3) 		216.8
(3) 		199
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187277_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6943
Sequence Type Exemplar sequence
Representative Public ID F25B5.2 NCBI
Target Description F25B5.2 /REP_DB=WormBase Gene ID /WP=CE01919 /TR=SW:Q09314 /GB=AAC46524.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065897(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065897 NCBI Caenorhabditis elegans F25B5.2 (F25B5.2) mRNA, complete cds. 11/11 None
SNAP00000007787 ENSEMBL cdna:SNAP chromosome:CEL140:III:5959249:5961977:1 11/11 None
GENEFINDER00000007793 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5959249:5961977:1 11/11 None
F25B5.2.1 ENSEMBL cdna:known chromosome:CEL140:III:5958720:5962225:1 gene:F25B5.2 11/11 None
F25B5.2.2 ENSEMBL cdna:known chromosome:CEL140:III:5959239:5962225:1 gene:F25B5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5959247-5961976(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F25B5.2
Entrez Gene 175845 Entrez gene
SwissProt Q09314 EMBL-EBI
WormBase CE01919 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498298 F25B5.2 [Caenorhabditis elegans] gb|AAC46524.1| Hypothetical protein F25B5.2 [Caenorhabditis elegans] sp|Q09314|YQT2_CAEEL Hypothetical protein F25B5.2 in chromosome III 0.0
blast CAE64346 Hypothetical protein CBG09033 [Caenorhabditis briggsae] 0.0
blast CAE64346 Hypothetical protein CBG09033 [Caenorhabditis briggsae] 1.0E-170
Sequence
Target Sequence 
>C. ELEGANS:187277_S_AT
ccactactgctgccacattcatggacagtgaactttcgaaaagggtgaaagaggagatcg
tgtcaaattatcgacctgatcgctgcggatcaatcaccgaatacgaaccggagctcgtgt
ttgtgatgcctgagcagccacaagcgagcttgaaaagtcttaatgactcggtttcctcta
atgctgacatcaaccttgatcaccttcggaatgttagaagtggcttgtgcaagtcgattg
aagaatggaaaaggatcacaaaaatgcgagaatcatctacaatgctcatttatccaatgt
tctgccaatcagaagaggaatgggattcaaaaggaaccatcgacgcaatattcctgatgc
tcgatggaatattaatgaatacaaaacgatggatgccgaacggaaaatgtattctcgctg
gggtgactccaaataatgtcatccttctacgcaacaagttcgatgaactcaaactgtcgg
ttgcaatctccgaatctgatgggccggcaagttcaaacgatgatttcgacactcaatcca
cagcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCACTACTGCTGCCACATTCATGGA 119 275 1709 Antisense
AATTATCGACCTGATCGCTGCGGAT 209 175 1774 Antisense
GAACCGGAGCTCGTGTTTGTGATGC 400 345 1813 Antisense
TGTGATGCCTGAGCAGCCACAAGCG 405 555 1830 Antisense
CTTAATGACTCGGTTTCCTCTAATG 438 219 1867 Antisense
TGACATCAACCTTGATCACCTTCGG 705 567 1893 Antisense
GAACCATCGACGCAATATTCCTGAT 89 345 2042 Antisense
AAATGTATTCTCGCTGGGGTGACTC 574 119 2113 Antisense
TAATGTCATCCTTCTACGCAACAAG 380 635 2142 Antisense
GAATCTGATGGGCCGGCAAGTTCAA 477 325 2200 Antisense
TGATTTCGACACTCAATCCACAGCG 205 565 2229 Antisense
Sequence Source Affymetrix Proprietary Database