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[187266_at] Potassium channel protein

Description F22B7.8 /REP_DB=WormBase Gene ID /WP=CE00161 /TR=SW:P34411 /GB=AAA65462.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 27      4grp_total > 0      6grp_cgc > 05      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 27.2
(0.2)
A 		5.2
(-3)
A 		8.1
(-2.6)
A 		46.1
(3)
A 		41
B 39.6
(1)
A 		7.8
(-3)
A 		55.3
(3)
A 		51.1
(2.5)
A 		48
C 14.7
(-1.7)
A 		5.1
(-3)
A 		20.2
(-1)
A 		50.7
(3)
A 		46
avr 27.2
(-0.1) 		6
(-3) 		27.9
(0) 		49.3
(3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 45.8
(3)
A 		1.9
(-3)
A 		8.3
(-2.1)
A 		5.5
(-2.5)
A 		15.5
(-1.1)
A 		7
(-2.3)
A 		44
B 7.9
(-1.8)
A 		4.6
(-2.6)
A 		9.6
(-1.3)
A 		5.5
(-2.4)
A 		3.2
(-3)
A 		25.8
(3)
A 		23
C 21.8
(-0.2)
A 		42
(3)
A 		7.6
(-2.5)
A 		4.4
(-3)
A 		4.4
(-3)
A 		15.4
(-1.2)
A 		38
avr 25.2
(3) 		16.2
(0.3) 		8.5
(-2) 		5.1
(-3) 		7.7
(-2.2) 		16.1
(0.3) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 9.9
(3)
A 		1.1
(-3)
A 		2.1
(-2.3)
A 		6.7
(0.8)
A 		9
B 12.6
(2.9)
A 		1.9
(-3)
A 		12.8
(3)
A 		10.9
(2)
A 		11
C 4.9
(-0.1)
A 		1.5
(-3)
A 		7
(1.7)
A 		8.5
(3)
A 		7
avr 9.1
(3) 		1.5
(-3) 		7.3
(1.6) 		8.7
(2.7) 		8

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 12
1 2 3 4 5 6 range
A 8.4
(3)
A 		0.7
(-3)
A 		1.8
(-2.1)
A 		1.4
(-2.5)
A 		4.7
(0.1)
A 		1.7
(-2.2)
A 		8
B 2.7
(-2)
A 		1.5
(-2.9)
A 		2.3
(-2.3)
A 		1.5
(-2.9)
A 		1.3
(-3)
A 		9.6
(3)
A 		8
C 8.1
(0.6)
A 		12.8
(3)
A 		1.9
(-2.5)
A 		0.9
(-3)
A 		1.4
(-2.7)
A 		5.1
(-0.9)
A 		12
avr 6.4
(3) 		5
(1.4) 		2
(-2.2) 		1.3
(-3) 		2.5
(-1.6) 		5.5
(1.9) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187266_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6430
Sequence Type Exemplar sequence
Representative Public ID F22B7.8 NCBI
Target Description F22B7.8 /REP_DB=WormBase Gene ID /WP=CE00161 /TR=SW:P34411 /GB=AAA65462.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066502(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066502 NCBI Caenorhabditis elegans TWiK family of potassium channels family member (twk-7) (twk-7) mRNA, complete cds. 11/11 None
GENEFINDER00000003844 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8634483:8647754:-1 11/11 None
SNAP00000003834 ENSEMBL cdna:SNAP chromosome:CEL140:III:8640766:8643625:-1 11/11 None
F22B7.7 ENSEMBL cdna:known chromosome:CEL140:III:8633981:8643642:-1 gene:F22B7.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8640760-8647749(-) 100.0 100.0
Public Domain and Genome References
Gene Title Potassium channel protein
Gene Symbol twk-7
Ensembl F22B7.7
Entrez Gene 192073 Entrez gene
SwissProt P34410 EMBL-EBI
WormBase CE37508 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1637725_AT dm
DROSGENOME1:144878_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6811 ion transport inferred from electronic annotation QuickGO AmiGO
6813 potassium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5267 potassium channel activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAU87822 Twik family of potassium channels protein 7 [Caenorhabditis elegans] ref|NP_498903.3| TWiK family of potassium channels family member (twk-7) [Caenorhabditis elegans] sp|P34410|TWK7_CAEEL TWiK family of potassium channels protein 0.0
blast CAE75020 Hypothetical protein CBG22924 [Caenorhabditis briggsae] 1.0E-155
blast AAU87822 Twik family of potassium channels protein 7 [Caenorhabditis elegans] ref|NP_498903.3| TWiK family of potassium channels family member (twk-7) [Caenorhabditis elegans] sp|P34410|TWK7_CAEEL TWiK family of potassium channels protein 2.0E-84
blast CAE75022 Hypothetical protein CBG22926 [Caenorhabditis briggsae] 1.0E-83
blast CAE75022 Hypothetical protein CBG22926 [Caenorhabditis briggsae] 6.0E-77
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 8.7E-19
Pfam IPR005821 EMBL-EBI Ion transport protein 8.7E-19
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498903.3 6 162-184,265-287,294-316,369-388,395-414,424-446
F22B7.7 6 162-184,265-287,294-316,369-388,395-414,424-446
GENEFINDER00000003844 7 279-301,380-402,407-428,483-505,510-529,541-563,578-600
SNAP00000003834 1 143-165
Sequence
Target Sequence 
>C. ELEGANS:187266_AT
atgacttcatcatctcgtggatatcaacgagttgactctagtggtgatggtggcagtcta
cttatggaagaggaaggtgataatccacatgaagcattgcttcatcgtaataacgatgat
tacgcttcaacataccaccacgtaggagttgaatatgacagctatgaagaacttgaacgg
catgagatggatgatcgagtcactgaaattccagaaggatttcatagacggcagagaagg
aacttgatttacggagatgacgtggcaacagatgaagagttgagagttttgaggagggaa
gctttattcggtgaagaagtaacaagagctggacttggaagagctgaatattatgaagat
agtcatttaattcacagttgtgataagagtggccatgaagatatcgacgatgaaagtgat
gatgaaagtaaagatgaagatgaagaagaagaggagactggaatcaggaagtttgctaaa
ctcgtcctacctcatgttgcacttgtacttctaacgtgtacatatacagtaattggagca
ttgatattttatagtgttgagcagccgcacgagca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGACTTCATCATCTCGTGGATATC 426 45 379 Antisense
ACGAGTTGACTCTAGTGGTGATGGT 571 93 405 Antisense
GTGGTGATGGTGGCAGTCTACTTAT 272 489 419 Antisense
GAAGCATTGCTTCATCGTAATAACG 62 341 469 Antisense
TGATTACGCTTCAACATACCACCAC 276 563 495 Antisense
AACATACCACCACGTAGGAGTTGAA 402 135 507 Antisense
AGAGTGGCCATGAAGATATCGACGA 318 69 764 Antisense
GAAGTTTGCTAAACTCGTCCTACCT 651 335 846 Antisense
CTACCTCATGTTGCACTTGTACTTC 384 225 865 Antisense
GTTGCACTTGTACTTCTAACGTGTA 116 439 874 Antisense
ATAGTGTTGAGCAGCCGCACGAGCA 643 19 929 Antisense
Sequence Source Affymetrix Proprietary Database