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[187265_at]

Description F30B5.4 /REP_DB=WormBase Gene ID /WP=CE04465 /TR=SW:Q19910 /GB=AAA83493.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 11      4grp_highest_8_8 > 15      4grp_high_8_8 > 06      6grp_highest_nonorm > 21      6grp_total > 13      4grp_total > 02      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1722
1 2 3 4 range
A 1303.2
(-1.9)
P 		1015.5
(-3)
P 		1401.8
(-1.5)
P 		2584
(3)
P 		1569
B 1090.4
(-2.6)
P 		1171
(-2.4)
P 		988.9
(-3)
P 		2711.1
(3)
P 		1722
C 1067.2
(-3)
P 		1450.1
(-1.2)
P 		1265.5
(-2.1)
P 		2360.3
(3)
P 		1293
avr 1153.6
(-3) 		1212.2
(-2.7) 		1218.7
(-2.7) 		2551.8
(3) 		1398

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 659
1 2 3 4 5 6 range
A 1457.1
(3)
P 		1458
(3)
P 		1178.7
(-0.1)
P 		1206.8
(0.2)
P 		1264.9
(0.8)
P 		925.4
(-3)
P 		533
B 1569.3
(3)
P 		1396.4
(1.2)
P 		1254.8
(-0.2)
P 		1422
(1.5)
P 		1164
(-1.1)
P 		977.7
(-3)
P 		592
C 1388
(2.2)
P 		1330.4
(1.6)
P 		1459.5
(3)
P 		1436.9
(2.8)
P 		1230.3
(0.5)
P 		910.7
(-3)
P 		549
avr 1471.5
(3) 		1394.9
(2.1) 		1297.7
(1) 		1355.2
(1.7) 		1219.7
(0.2) 		937.9
(-3) 		534
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 357
1 2 3 4 range
A 474.5
(3)
P 		220.4
(-3)
P 		366.5
(0.4)
P 		378.2
(0.7)
P 		254
B 347.7
(-1)
P 		289.2
(-2)
P 		229.1
(-3)
P 		577.7
(3)
P 		349
C 353.3
(-3)
P 		416.9
(1.3)
P 		441.1
(3)
P 		398
(0.1)
P 		88
avr 391.8
(0.5) 		308.8
(-3) 		345.6
(-1.5) 		451.3
(3) 		143

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 321
1 2 3 4 5 6 range
A 268.6
(-2.1)
P 		518.5
(3)
P 		256.7
(-2.4)
P 		306.1
(-1.3)
P 		381.7
(0.2)
P 		225.3
(-3)
P 		293
B 546
(3)
P 		464.8
(1)
P 		304.7
(-3)
P 		376.6
(-1.2)
P 		472.9
(1.2)
P 		364.6
(-1.5)
P 		241
C 514.5
(3)
P 		405.9
(0.1)
P 		371.5
(-0.8)
P 		289
(-3)
P 		398.1
(-0.1)
P 		304.9
(-2.6)
P 		226
avr 443
(2.3) 		463.1
(3) 		311
(-2.5) 		323.9
(-2.1) 		417.6
(1.3) 		298.3
(-3) 		165
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187265_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10521
Sequence Type Exemplar sequence
Representative Public ID F30B5.4 NCBI
Target Description F30B5.4 /REP_DB=WormBase Gene ID /WP=CE04465 /TR=SW:Q19910 /GB=AAA83493.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068113(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068113 NCBI Caenorhabditis elegans F30B5.4 (F30B5.4) mRNA, complete cds. 11/11 None
SNAP00000023152 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4216317:4218063:1 11/11 None
GENEFINDER00000023162 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4216292:4218063:1 11/11 None
F30B5.4.2 ENSEMBL cdna:known chromosome:CEL140:IV:4205968:4218184:1 gene:F30B5.4 11/11 None
F30B5.4.1 ENSEMBL cdna:known chromosome:CEL140:IV:4213570:4218224:1 gene:F30B5.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4216653-4218069(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F30B5.4
Entrez Gene 177185 Entrez gene
SwissProt Q19910 EMBL-EBI
WormBase CE28552 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605258_AT similar to Protein C8orf1 (hT41) cfa
CANINE_2:CFAAFFX.14159.1.S1_AT similar to Protein C8orf1 (hT41) cfa
CHICKEN:GGA.13993.1.S1_S_AT similar to cDNA sequence BC010311 gga
CHICKEN:GGAAFFX.24807.1.S1_AT similar to cDNA sequence BC010311 gga
CHICKEN:GGAAFFX.24807.1.S1_S_AT similar to cDNA sequence BC010311 gga
HG-U133_PLUS_2:204024_AT chromosome 8 open reading frame 1 hs
HG-U133A:204024_AT chromosome 8 open reading frame 1 hs
HG-U133A_2:204024_AT chromosome 8 open reading frame 1 hs
HU35KSUBC:RC_AA293495_AT chromosome 8 open reading frame 1 hs
HU35KSUBC:RC_AA256275_AT chromosome 8 open reading frame 1 hs
U133_X3P:G4757889_3P_AT chromosome 8 open reading frame 1 hs
U133_X3P:HS2.40539.2.S1_3P_S_AT chromosome 8 open reading frame 1 hs
HG-U133_PLUS_2:1554414_A_AT chromosome 8 open reading frame 1 hs
HG-U95AV2:41554_AT chromosome 8 open reading frame 1 hs
HG-U95E:72999_F_AT chromosome 8 open reading frame 1 hs
HU35KSUBC:RC_N25650_AT Chromosome 8 open reading frame 1 hs
HG-U133A_2:214161_AT Chromosome 8 open reading frame 1 hs
HG-U133_PLUS_2:214161_AT Chromosome 8 open reading frame 1 hs
HG-U133A:214161_AT Chromosome 8 open reading frame 1 hs
HG-U133A:41553_AT Chromosome 8 open reading frame 1 hs
HG-U133A_2:41553_AT Chromosome 8 open reading frame 1 hs
HG-FOCUS:41553_AT Chromosome 8 open reading frame 1 hs
HG-U133_PLUS_2:41553_AT Chromosome 8 open reading frame 1 hs
HG-U95AV2:41553_AT Chromosome 8 open reading frame 1 hs
HU35KSUBC:RC_AA252791_AT Chromosome 8 open reading frame 1 hs
HU35KSUBC:RC_AA027049_AT Chromosome 8 open reading frame 1 hs
U133_X3P:HS.40539.1.A1_3P_AT Chromosome 8 open reading frame 1 hs
U133_X3P:HS.40539.1.A1_3P_S_AT Chromosome 8 open reading frame 1 hs
MOE430A:1424290_AT cDNA sequence BC010311 mm
MOUSE430A_2:1424290_AT cDNA sequence BC010311 mm
MOUSE430_2:1424290_AT cDNA sequence BC010311 mm
MOUSE430_2:1441568_AT cDNA sequence BC010311 mm
MOE430B:1441568_AT cDNA sequence BC010311 mm
MG-U74BV2:163765_AT cDNA sequence BC010311 mm
MOE430A:1424289_AT cDNA sequence BC010311 mm
MOUSE430A_2:1424289_AT cDNA sequence BC010311 mm
MOUSE430_2:1424289_AT cDNA sequence BC010311 mm
RAE230B:1380553_AT similar to Protein C8orf1 (hT41) (predicted) rn
RAT230_2:1380553_AT similar to Protein C8orf1 (hT41) (predicted) rn
Protein Similarities
Method ID Description E-Value
blast NP_500514 F30B5.4 [Caenorhabditis elegans] sp|Q19910|YKYL_CAEEL Hypothetical protein F30B5.4 in chromosome IV 0.0
blast CAE61458 Hypothetical protein CBG05349 [Caenorhabditis briggsae] 0.0
blast T29571 hypothetical protein F30B5.4 - Caenorhabditis elegans 0.0
Sequence
Target Sequence 
>C. ELEGANS:187265_AT
cacctgctcaccggtgattgtcgttggggacggagtgtcgtctgttgattgcgtacgaca
ctgtctcgagagagatataccagtggttcatgtgattcggaggactttgagggagcttag
aaatgtaatgctatcccgcctctcaccaatccactactcggaatacaccgacatctatcg
aatgatgatcggccgctccgttcacaagaactaccaacgtgtgctcgacgcaagcatcac
gtccgtctcgaagatccacgtggaaatcacgacaggcagcaaccgggagatcatcgaaat
gccctactcaacagtagccgtgtgcatcggtcgggaatctcatttctccacggtcttcga
gaacgcacacacattcctcgactaccagagcgaacaggatgagacgctgttcgccgttgg
agcatacgctggagaccattttgtacggttcctggttggaggatgtcttcgggtcgccca
acatatcactggagctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACCTGCTCACCGGTGATTGTCGTT 286 201 558 Antisense
GGAGTGTCGTCTGTTGATTGCGTAC 696 515 589 Antisense
CAAGAACTACCAACGTGTGCTCGAC 649 183 762 Antisense
TGTGCTCGACGCAAGCATCACGTCC 390 557 777 Antisense
GATCATCGAAATGCCCTACTCAACA 659 415 846 Antisense
AACAGTAGCCGTGTGCATCGGTCGG 118 135 867 Antisense
TCGGTCGGGAATCTCATTTCTCCAC 56 599 884 Antisense
CACATTCCTCGACTACCAGAGCGAA 261 197 927 Antisense
TTCGCCGTTGGAGCATACGCTGGAG 547 697 967 Antisense
GGAGACCATTTTGTACGGTTCCTGG 394 519 988 Antisense
GTCGCCCAACATATCACTGGAGCTG 131 475 1030 Antisense
Sequence Source Affymetrix Proprietary Database