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[187261_s_at]

Description B0228.7 /REP_DB=WormBase Gene ID /WP=CE01747 /TR=SW:Q09438 /GB=AAC38810.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 74      4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_highest_nonorm > 55      6grp_total > 64      4grp_total > 40      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1523
1 2 3 4 range
A 1785.1
(-3)
P 		2557.6
(0.6)
P 		3078
(3)
P 		2252.9
(-0.8)
P 		1293
B 1852.8
(-3)
P 		2592
(0.4)
P 		3144.9
(3)
P 		2787.9
(1.3)
P 		1292
C 1912.6
(-3)
P 		2541.5
(-0.3)
P 		3308.2
(3)
P 		2774.6
(0.7)
P 		1396
avr 1850.2
(-3) 		2563.7
(0.2) 		3177
(3) 		2605.1
(0.4) 		1327

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 961
1 2 3 4 5 6 range
A 1124.1
(-3)
P 		1517
(-0.4)
P 		2037.2
(3)
P 		1967.1
(2.5)
P 		1290.3
(-1.9)
P 		1628.2
(0.3)
P 		913
B 1420.6
(-3)
P 		1527.6
(-1.5)
P 		1624.4
(-0.2)
P 		1700.7
(0.8)
P 		1614
(-0.4)
P 		1860.8
(3)
P 		440
C 1286.5
(-3)
P 		1613.6
(-0.5)
P 		1805.2
(0.9)
P 		2005.5
(2.4)
P 		1408.4
(-2.1)
P 		2084.6
(3)
P 		798
avr 1277.1
(-3) 		1552.7
(-0.3) 		1822.3
(2.3) 		1891.1
(3) 		1437.6
(-1.4) 		1857.9
(2.7) 		614
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 823
1 2 3 4 range
A 649.9
(1)
P 		555.2
(-0.2)
P 		804.7
(3)
P 		329.8
(-3)
P 		475
B 590.9
(-3)
P 		640.2
(-0.9)
P 		728.7
(3)
P 		594.1
(-2.9)
P 		138
C 633.1
(-1.6)
P 		730.7
(-0.7)
P 		1153
(3)
P 		467.8
(-3)
P 		685
avr 624.6
(-0.8) 		642
(-0.5) 		895.5
(3) 		463.9
(-3) 		432

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 491
1 2 3 4 5 6 range
A 207.2
(-3)
P 		539.5
(3)
P 		443.7
(1.3)
P 		498.9
(2.3)
P 		389.3
(0.3)
P 		396.4
(0.4)
P 		332
B 494.3
(-1)
P 		508.5
(-0.7)
P 		394.5
(-3)
P 		450.5
(-1.9)
P 		655.8
(2.2)
P 		693.9
(3)
P 		299
C 476.9
(-1.5)
P 		492.4
(-1.2)
P 		459.4
(-1.9)
P 		403.3
(-3)
P 		455.8
(-1.9)
P 		697.8
(3)
P 		295
avr 392.8
(-3) 		513.5
(0.6) 		432.5
(-1.8) 		450.9
(-1.3) 		500.3
(0.2) 		596
(3) 		203
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187261_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5699
Sequence Type Exemplar sequence
Representative Public ID B0228.7 NCBI
Target Description B0228.7 /REP_DB=WormBase Gene ID /WP=CE01747 /TR=SW:Q09438 /GB=AAC38810.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063228(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063228 NCBI Caenorhabditis elegans B0228.7 (B0228.7) mRNA, complete cds. 11/11 None
SNAP00000020607 ENSEMBL cdna:SNAP chromosome:CEL140:II:7757149:7758299:-1 11/11 None
GENEFINDER00000020738 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7757056:7758299:-1 11/11 None
B0228.7 ENSEMBL cdna:known chromosome:CEL140:II:7757133:7758311:-1 gene:B0228.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7757145-7758296(-) 99.3 100.0
Public Domain and Genome References
Gene Title
Ensembl B0228.7
Entrez Gene 174252 Entrez gene
SwissProt Q09438 EMBL-EBI
WormBase CE34630 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1597406_S_AT similar to S-methyl-5-thioadenosine phosphorylase (5-methylthioadenosine phosphorylase) (MTA phosphorylase) (MTAPase) cfa
CANINE_2:CFA.15125.1.S1_AT similar to S-methyl-5-thioadenosine phosphorylase (5-methylthioadenosine phosphorylase) (MTA phosphorylase) (MTAPase) cfa
CANINE_2:CFAAFFX.3413.1.S1_S_AT similar to S-methyl-5-thioadenosine phosphorylase (5-methylthioadenosine phosphorylase) (MTA phosphorylase) (MTAPase) cfa
DROSOPHILA_2:1634972_AT dm
DROSGENOME1:153128_AT dm
CHICKEN:GGAAFFX.13180.1.S1_S_AT methylthioadenosine phosphorylase gga
HG-U133_PLUS_2:231984_AT methylthioadenosine phosphorylase hs
HG-U133B:231984_AT methylthioadenosine phosphorylase hs
HG-U133_PLUS_2:216685_S_AT methylthioadenosine phosphorylase hs
HG-U133A_2:216685_S_AT methylthioadenosine phosphorylase hs
HG-U133A:216685_S_AT methylthioadenosine phosphorylase hs
HG-U133_PLUS_2:204956_AT methylthioadenosine phosphorylase hs
HG-U133A:204956_AT methylthioadenosine phosphorylase hs
HG-FOCUS:204956_AT methylthioadenosine phosphorylase hs
HG-U133A_2:204956_AT methylthioadenosine phosphorylase hs
HG-U133_PLUS_2:211363_S_AT methylthioadenosine phosphorylase hs
HG-U133A:211363_S_AT methylthioadenosine phosphorylase hs
HG-U133A_2:211363_S_AT methylthioadenosine phosphorylase hs
HG-U133_PLUS_2:211364_AT methylthioadenosine phosphorylase hs
HG-U133A:211364_AT methylthioadenosine phosphorylase hs
HG-U133A_2:211364_AT methylthioadenosine phosphorylase hs
HU35KSUBD:RC_AA488387_S_AT methylthioadenosine phosphorylase hs
HUGENEFL:U22233_AT methylthioadenosine phosphorylase hs
U133_X3P:G6006025_3P_AT methylthioadenosine phosphorylase hs
U133_X3P:G4378719_3P_AT methylthioadenosine phosphorylase hs
U133_X3P:HS.152817.1.S1_3P_X_AT methylthioadenosine phosphorylase hs
U133_X3P:HS.152817.1.S1_3P_A_AT methylthioadenosine phosphorylase hs
U133_X3P:HS.118190.0.S1_3P_AT methylthioadenosine phosphorylase hs
HG-U95AV2:38150_AT methylthioadenosine phosphorylase hs
HG-U95E:76183_AT methylthioadenosine phosphorylase hs
HG-U133A_2:217134_AT methylthioadenosine phosphorylase hs
HG-U133_PLUS_2:217134_AT methylthioadenosine phosphorylase hs
HG-U133A:217134_AT methylthioadenosine phosphorylase hs
HG-U95B:48605_AT Methylthioadenosine phosphorylase hs
HU35KSUBD:RC_R89260_AT Methylthioadenosine phosphorylase hs
MG-U74BV2:108535_AT methylthioadenosine phosphorylase mm
MOE430A:1451345_AT methylthioadenosine phosphorylase mm
MOUSE430A_2:1451345_AT methylthioadenosine phosphorylase mm
MOUSE430_2:1451345_AT methylthioadenosine phosphorylase mm
MOE430A:1451346_AT methylthioadenosine phosphorylase mm
MOUSE430A_2:1451346_AT methylthioadenosine phosphorylase mm
MOUSE430_2:1451346_AT methylthioadenosine phosphorylase mm
MOE430A:1424426_AT methylthioadenosine phosphorylase mm
MOUSE430A_2:1424426_AT methylthioadenosine phosphorylase mm
MOUSE430_2:1424426_AT methylthioadenosine phosphorylase mm
MOE430A:1424425_A_AT methylthioadenosine phosphorylase mm
MOUSE430A_2:1424425_A_AT methylthioadenosine phosphorylase mm
MOUSE430_2:1424425_A_AT methylthioadenosine phosphorylase mm
MU19KSUBC:TC35066_AT Methylthioadenosine phosphorylase, mRNA (cDNA clone MGC:6590 IMAGE:3485422) mm
MU19KSUBC:TC38116_AT Methylthioadenosine phosphorylase, mRNA (cDNA clone MGC:6590 IMAGE:3485422) mm
YEAST_2:1778120_AT Methylthioadenosine phosphorylase (MTAP), catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression Sc
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4645 phosphorylase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC38810 Hypothetical protein B0228.7 [Caenorhabditis elegans] ref|NP_495629.2| B0228.7 [Caenorhabditis elegans] sp|Q09438|MTAP_CAEEL Putative S-methyl-5-thioadenosine phosphorylase (5'-methylthioadenosine phosphorylase) (MTA phosphorylase) (MTAPase) 1.0E-166
blast CAE67466 Hypothetical protein CBG12968 [Caenorhabditis briggsae] 1.0E-141
blast EAL25138 GA18442-PA [Drosophila pseudoobscura] 1.0E-77
Protein Domains
Database ID Description E-Value
Pfam IPR001369 EMBL-EBI Purine (and other) phosphorylase, family 2 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187261_S_AT
cagcatgcggatctctccaggaaaacgtcgaaccaggacatttactgttcccagactcag
taacaactgggcgccaaagcacattcttcgatggaagctacgatcaagctccaggagtct
gtcatattcaagctcatccaacgtacaatgagaaactgagacaggttctaatttcaactg
ctgaaagatgtcaacttgtccatcatagaaccggtttcggagtgtgcattgaaggtccac
gattctcaacaaaagctgagagcatggtgtttaagtcatggggagcaagcttagttaaca
tgacaatgatgccagagtgtattcttgccaaggaactcggtattccatacgctaccacag
ctcttgtcactgactacgattgctggaaagaagaagatcatgtgactgcatcatctgtaa
tgaaagtgtttgcagcaaatgttgaaaaggccaagacattgttcgtagaagctgttggag
aaatcggaaaaattgattggagcgcagaaattcttaaactcaagaccgaagctcgcgaat
ctgtcatgatttcaccggacgtcgtc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGCATGCGGATCTCTCCAGGAAAA 279 203 266 Antisense
CAGGACATTTACTGTTCCCAGACTC 211 207 299 Antisense
GGCGCCAAAGCACATTCTTCGATGG 177 543 335 Antisense
GCTACGATCAAGCTCCAGGAGTCTG 73 303 362 Antisense
AGTCTGTCATATTCAAGCTCATCCA 531 61 381 Antisense
GTCAACTTGTCCATCATAGAACCGG 638 463 455 Antisense
GTGCATTGAAGGTCCACGATTCTCA 226 479 489 Antisense
GATGCCAGAGTGTATTCTTGCCAAG 442 415 573 Antisense
CTTGTCACTGACTACGATTGCTGGA 584 217 628 Antisense
ACCGAAGCTCGCGAATCTGTCATGA 28 89 790 Antisense
TGTCATGATTTCACCGGACGTCGTC 560 557 807 Antisense
Sequence Source Affymetrix Proprietary Database