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[187258_at]

Description C54D1.5 /REP_DB=WormBase Gene ID /WP=CE06981 /TR=SW:Q18823 /GB=AAC48152.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 46      4grp_total > 52      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 164.1
(2.5)
A 		176.8
(3)
A 		129.7
(1.1)
A 		26.5
(-3)
A 		150
B 167
(3)
M 		157.9
(2.6)
A 		166.4
(3)
A 		43.2
(-3)
A 		124
C 179.9
(2.2)
P 		197.5
(3)
A 		126.6
(-0.2)
A 		65.2
(-3)
A 		132
avr 170.3
(2.7) 		177.4
(3) 		140.9
(1.3) 		45
(-3) 		132

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 80
1 2 3 4 5 6 range
A 91.8
(2.2)
A 		77.8
(1.2)
A 		75.9
(1)
A 		53.3
(-0.7)
A 		21.9
(-3)
A 		102.1
(3)
A 		80
B 81.9
(3)
A 		58.3
(0.4)
A 		41.9
(-1.4)
A 		27
(-3)
A 		34.7
(-2.2)
A 		77.8
(2.6)
A 		55
C 89.4
(1.6)
A 		89.5
(1.7)
A 		101.3
(3)
A 		92.1
(1.9)
A 		48.8
(-3)
A 		93.9
(2.2)
A 		53
avr 87.7
(2.6) 		75.2
(1.3) 		73
(1) 		57.5
(-0.6) 		35.1
(-3) 		91.3
(3) 		56
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 59.7
(3)
A 		38.4
(0.7)
A 		33.9
(0.2)
A 		3.9
(-3)
A 		56
B 53.3
(3)
M 		39
(1.1)
A 		38.5
(1)
A 		9.2
(-3)
A 		44
C 59.6
(3)
P 		56.8
(2.7)
A 		44.1
(1.1)
A 		11
(-3)
A 		49
avr 57.5
(3) 		44.7
(1.4) 		38.8
(0.7) 		8
(-3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 16.9
(-0.1)
A 		27.7
(3)
A 		16.5
(-0.2)
A 		13.5
(-1)
A 		6.6
(-3)
A 		24.8
(2.2)
A 		21
B 28.5
(2.9)
A 		19.4
(0.4)
A 		10.2
(-2.2)
A 		7.2
(-3)
A 		14.1
(-1.1)
A 		29
(3)
A 		22
C 33.1
(3)
A 		27.3
(1)
A 		25.8
(0.5)
A 		18.5
(-2.1)
A 		15.8
(-3)
A 		31.4
(2.4)
A 		17
avr 26.2
(2.2) 		24.8
(1.7) 		17.5
(-1) 		13.1
(-2.7) 		12.2
(-3) 		28.4
(3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187258_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15997
Sequence Type Exemplar sequence
Representative Public ID C54D1.5 NCBI
Target Description C54D1.5 /REP_DB=WormBase Gene ID /WP=CE06981 /TR=SW:Q18823 /GB=AAC48152.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076803(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076803 NCBI Caenorhabditis elegans C54D1.5 (C54D1.5) mRNA, complete cds. 11/11 None
SNAP00000023886 ENSEMBL cdna:SNAP chromosome:CEL140:X:7144883:7151134:-1 9/11 None
GENEFINDER00000023893 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:7144883:7151089:-1 9/11 None
C54D1.5 ENSEMBL cdna:known chromosome:CEL140:X:7144822:7151089:-1 gene:C54D1.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:7144881-7151088(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C54D1.5
Entrez Gene 180980 Entrez gene
SwissProt Q18823 EMBL-EBI
WormBase CE30916 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1590151_AT similar to Laminin gamma-1 chain precursor (Laminin B2 chain) cfa
CANINE:1589041_AT similar to Laminin gamma-1 chain precursor (Laminin B2 chain) cfa
CANINE_2:CFA.15747.1.S1_AT similar to Laminin gamma-1 chain precursor (Laminin B2 chain) cfa
CANINE_2:CFA.3306.1.S1_S_AT similar to Laminin gamma-1 chain precursor (Laminin B2 chain) cfa
CANINE_2:CFAAFFX.20472.1.S1_S_AT similar to Laminin gamma-1 chain precursor (Laminin B2 chain) cfa
DROSOPHILA_2:1632666_AT Laminin B2 dm
DROSGENOME1:153856_AT Laminin B2 dm
CHICKEN:GGA.1330.1.S1_S_AT laminin, gamma 1 (formerly LAMB2) gga
CHICKEN:GGA.16971.1.S1_AT laminin, gamma 1 (formerly LAMB2) gga
CHICKEN:GGAAFFX.12913.1.S1_AT laminin, gamma 1 (formerly LAMB2) gga
HG-U133_PLUS_2:200770_S_AT laminin, gamma 1 (formerly LAMB2) hs
HG-U133A_2:200770_S_AT laminin, gamma 1 (formerly LAMB2) hs
HG-U133A:200770_S_AT laminin, gamma 1 (formerly LAMB2) hs
HG-U133_PLUS_2:200771_AT laminin, gamma 1 (formerly LAMB2) hs
HG-FOCUS:200771_AT laminin, gamma 1 (formerly LAMB2) hs
HG-U133A_2:200771_AT laminin, gamma 1 (formerly LAMB2) hs
HG-U133A:200771_AT laminin, gamma 1 (formerly LAMB2) hs
HG-U95AV2:232_AT laminin, gamma 1 (formerly LAMB2) hs
HC-G110:232_AT laminin, gamma 1 (formerly LAMB2) hs
HU35KSUBC:RC_AA037058_S_AT laminin, gamma 1 (formerly LAMB2) hs
HUGENEFL:M55210_AT laminin, gamma 1 (formerly LAMB2) hs
U133_X3P:G9845497_3P_AT laminin, gamma 1 (formerly LAMB2) hs
U133_X3P:G186916_3P_A_AT laminin, gamma 1 (formerly LAMB2) hs
HG-U133_PLUS_2:243591_AT Laminin, gamma 1 (formerly LAMB2) hs
HG-U133B:243591_AT Laminin, gamma 1 (formerly LAMB2) hs
HG-U95B:52430_R_AT Laminin, gamma 1 (formerly LAMB2) hs
HG-U95D:75708_AT Laminin, gamma 1 (formerly LAMB2) hs
HG-U95E:84551_AT Laminin, gamma 1 (formerly LAMB2) hs
HU35KSUBB:RC_R27606_AT Laminin, gamma 1 (formerly LAMB2) hs
HU35KSUBD:RC_AA443985_AT Laminin, gamma 1 (formerly LAMB2) hs
U133_X3P:HS.293662.0.A1_3P_AT Laminin, gamma 1 (formerly LAMB2) hs
MU11KSUBA:J02930_S_AT laminin, gamma 1 mm
MG-U74AV2:102404_AT laminin, gamma 1 mm
MG-U74AV2:161706_F_AT laminin, gamma 1 mm
MOE430A:1423886_AT laminin, gamma 1 mm
MOUSE430A_2:1423886_AT laminin, gamma 1 mm
MOUSE430_2:1423886_AT laminin, gamma 1 mm
MOE430A:1423885_AT laminin, gamma 1 mm
MOUSE430A_2:1423885_AT laminin, gamma 1 mm
MOUSE430_2:1423885_AT laminin, gamma 1 mm
MU19KSUBB:TC27250_AT Laminin, gamma 1, mRNA (cDNA clone IMAGE:5708226) mm
MU19KSUBB:TC32460_AT Laminin, gamma 1, mRNA (cDNA clone IMAGE:5708226) mm
MU19KSUBB:TC32460_G_AT Laminin, gamma 1, mRNA (cDNA clone IMAGE:5708226) mm
RG-U34A:X94551_AT laminin, gamma 1 rn
RG-U34C:RC_AI071644_S_AT laminin, gamma 1 rn
RAE230A:1370993_AT laminin, gamma 1 rn
RAT230_2:1370993_AT laminin, gamma 1 rn
RAE230A:1371322_AT Laminin, gamma 1 rn
RAT230_2:1371322_AT Laminin, gamma 1 rn
RAE230B:1396877_AT Laminin, gamma 1 rn
RAT230_2:1396877_AT Laminin, gamma 1 rn
RAE230B:1396879_AT Laminin, gamma 1 rn
RAT230_2:1396879_AT Laminin, gamma 1 rn
RG-U34B:RC_AI009131_AT Laminin, gamma 1 rn
RG-U34B:RC_AI059685_AT Laminin, gamma 1 rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
5578 extracellular matrix (sensu Metazoa) inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC48152 Hypothetical protein C54D1.5 [Caenorhabditis elegans] ref|NP_509204.2| C54D1.5 [Caenorhabditis elegans] sp|Q18823|LAML1_CAEEL Laminin-like protein C54D1.5 precursor 0.0
blast T28811 hypothetical protein C54D1.5 - Caenorhabditis elegans 0.0
blast CAE65849 Hypothetical protein CBG10982 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.3.1.Two-domain cytochrome c All alpha proteins; Cytochrome c; Cytochrome c; Two-domain cytochrome c 1.20000004768372
Pfam IPR008211 EMBL-EBI Laminin, N-terminal 5.6E-114
Pfam IPR000034 EMBL-EBI Laminin B 9.5E-32
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 9.7E-21
Pfam IPR008211 EMBL-EBI Laminin, N-terminal 5.6E-114
Pfam IPR000034 EMBL-EBI Laminin B 9.5E-32
Pfam IPR002049 EMBL-EBI Laminin-type EGF-like domain 9.7E-21
Sequence
Target Sequence 
>C. ELEGANS:187258_AT
ttgttgactctgtatcgactgctgataagactttgaaggatgccgaagccgctttacaag
ttttggaagagttcggcgccaagatcgaaaaatctcgaaatgatgctgtcgcagaatttg
ctggagtagaaggaatcaatcagagacttgatgatattattgatgctcaagataaaagaa
gaaactctctcccaattgataagcagtttgttatcgactacagaaaatccgctgatgttc
ttctcaatgaaactcacgctcttgccgacagatacaaggatattattcattcagatgttg
atacgcgagattccaccgaagctgttcagtatgatattgaacaattaatggaagaactca
cagattcaaatgaaaacctgcagtattacaagaaacaagccgaagacgataagcaaatgg
ctactgaagcggtccgcaagcaactcttgccaagaattctgccatcgaagcaaatgctac
cattctcgctgaggaagatgaaatcaagaaaatcatcaattctttgggttttgaacctag
aggaaatccgtgacaacttgccaaccaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTTGACTCTGTATCGACTGCTGA 346 707 4076 Antisense
GAAGGATGCCGAAGCCGCTTTACAA 675 333 4110 Antisense
GAAACTCTCTCCCAATTGATAAGCA 517 357 4256 Antisense
CAGAAAATCCGCTGATGTTCTTCTC 116 205 4296 Antisense
CTCAATGAAACTCACGCTCTTGCCG 433 227 4318 Antisense
GATGTTGATACGCGAGATTCCACCG 162 409 4369 Antisense
GATTCCACCGAAGCTGTTCAGTATG 424 433 4384 Antisense
TGAAGCGGTCCGCAAGCAACTCTTG 104 573 4500 Antisense
ACTCTTGCCAAGAATTCTGCCATCG 656 99 4518 Antisense
GAAGCAAATGCTACCATTCTCGCTG 458 341 4542 Antisense
ATCCGTGACAACTTGCCAACCAAAT 158 37 4621 Antisense
Sequence Source Affymetrix Proprietary Database