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[187254_at]

Description ZK353.2 /REP_DB=WormBase Gene ID /WP=CE00386 /TR=SW:P34625 /GB=AAA28199.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 25      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 19.8
(-3)
A 		52.2
(3)
P 		29.5
(-1.2)
A 		50.3
(2.6)
A 		32
B 35.3
(-3)
A 		69.7
(1.8)
A 		37.3
(-2.7)
A 		78.5
(3)
M 		43
C 5
(-3)
A 		33.6
(-0)
A 		50.9
(1.8)
P 		62.3
(3)
A 		57
avr 20
(-3) 		51.8
(1.4) 		39.2
(-0.4) 		63.7
(3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 39.3
(-3)
A 		61.9
(1.7)
P 		59
(1.1)
A 		50.1
(-0.7)
P 		61.6
(1.7)
P 		68
(3)
P 		29
B 27.1
(-1.6)
A 		65.6
(1.1)
P 		7.6
(-3)
A 		44.3
(-0.4)
P 		93.1
(3)
P 		49.8
(-0)
P 		86
C 18.5
(-3)
A 		54.8
(1.5)
A 		46
(0.4)
P 		67
(3)
P 		60.3
(2.2)
P 		64.2
(2.7)
P 		49
avr 28.3
(-3) 		60.8
(1.5) 		37.5
(-1.7) 		53.8
(0.5) 		71.7
(3) 		60.7
(1.5) 		43
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 7.2
(-3)
A 		11.3
(3)
P 		7.7
(-2.3)
A 		7.4
(-2.7)
A 		4
B 11.3
(-1.1)
A 		17.2
(3)
A 		8.6
(-3)
A 		16.7
(2.7)
M 		9
C 1.6
(-3)
A 		9.7
(0)
A 		17.8
(3)
P 		10.5
(0.3)
A 		16
avr 6.7
(-3) 		12.7
(3) 		11.4
(1.7) 		11.5
(1.8) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 7.2
(-3)
A 		22
(3)
P 		12.8
(-0.7)
A 		12.7
(-0.8)
P 		18.6
(1.6)
P 		16.6
(0.8)
P 		15
B 9.4
(-1.7)
A 		21.8
(0.3)
P 		1.8
(-3)
A 		11.7
(-1.4)
P 		37.8
(3)
P 		18.6
(-0.2)
P 		36
C 6.8
(-3)
A 		16.7
(1)
A 		11.7
(-1)
P 		13.5
(-0.3)
P 		19.5
(2.2)
P 		21.5
(3)
P 		15
avr 7.8
(-3) 		20.2
(1.3) 		8.8
(-2.7) 		12.6
(-1.4) 		25.3
(3) 		18.9
(0.8) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187254_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8069
Sequence Type Exemplar sequence
Representative Public ID ZK353.2 NCBI
Target Description ZK353.2 /REP_DB=WormBase Gene ID /WP=CE00386 /TR=SW:P34625 /GB=AAA28199.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066452(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066452 NCBI Caenorhabditis elegans ZK353.2 (ZK353.2) mRNA, complete cds. 11/11 None
SNAP00000044094 ENSEMBL cdna:SNAP chromosome:CEL140:III:8398639:8399051:1 11/11 None
GENEFINDER00000044108 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8398639:8399051:1 11/11 None
ZK353.2 ENSEMBL cdna:known chromosome:CEL140:III:8398639:8399051:1 gene:ZK353.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8398634-8399047(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK353.2
Entrez Gene 191286 Entrez gene
SwissProt P34625 EMBL-EBI
WormBase CE00386 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA28199 Hypothetical protein ZK353.2 [Caenorhabditis elegans] ref|NP_498853.1| ZK353.2 [Caenorhabditis elegans] sp|P34625|YOJ2_CAEEL Hypothetical protein ZK353.2 in chromosome III 6.0E-23
blast CAE71266 Hypothetical protein CBG18148 [Caenorhabditis briggsae] 2.0E-22
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498853.1 2 7-29,68-90
ZK353.2 2 7-29,68-90
SNAP00000044094 2 7-29,68-90
GENEFINDER00000044108 2 7-29,68-90
Sequence
Target Sequence 
>C. ELEGANS:187254_AT
gtggatacggtaattgtggtggtggaatacttggatcagttcttggaatggctggtggaa
tggctggaggatacggtggatatggtggatatggaggttatggaagatgtggagcagata
atgtgttttatcgttggcgttgttgtgactacagtccttatgaatgttgtatacaattgg
agacatgggttgtagtatttcttgtcatattcataattggattcttcgtatgtctttgtg
cttgtctagccgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGATACGGTAATTGTGGTGGTGG 204 487 29 Antisense
GTGGTGGTGGAATACTTGGATCAGT 612 477 44 Antisense
GTGGAATGGCTGGAGGATACGGTGG 644 487 83 Antisense
GATGTGGAGCAGATAATGTGTTTTA 347 409 134 Antisense
AATGTGTTTTATCGTTGGCGTTGTT 147 163 148 Antisense
GTTGGCGTTGTTGTGACTACAGTCC 9 435 161 Antisense
GTGACTACAGTCCTTATGAATGTTG 668 481 173 Antisense
GGGTTGTAGTATTTCTTGTCATATT 159 495 215 Antisense
GTCATATTCATAATTGGATTCTTCG 422 467 232 Antisense
GGATTCTTCGTATGTCTTTGTGCTT 519 509 247 Antisense
ATGTCTTTGTGCTTGTCTAGCCGGA 156 49 258 Antisense
Sequence Source Affymetrix Proprietary Database