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[187249_s_at]

Description K04H4.6B /REP_DB=WormBase Gene ID /WP=CE00251 /TR=SW:P34508 /GB=CAA81586.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 21      4grp_high_8_8 > 50      6grp_highest_nonorm > 61      6grp_total > 32      4grp_total > 53      6grp_cgc > 71      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 367
1 2 3 4 range
A 332.4
(3)
P 		261.3
(1.1)
P 		245.3
(0.7)
P 		106.3
(-3)
P 		226
B 316.3
(3)
P 		285.4
(2.3)
P 		271
(1.9)
P 		66.7
(-3)
A 		250
C 423.5
(3)
P 		289.8
(0.8)
P 		237.1
(-0)
P 		56.3
(-3)
A 		367
avr 357.4
(3) 		278.8
(1.3) 		251.1
(0.7) 		76.4
(-3) 		281

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 417
1 2 3 4 5 6 range
A 521.7
(3)
P 		472.2
(2)
P 		509.5
(2.8)
P 		382.4
(0.2)
P 		223.2
(-3)
P 		227.6
(-2.9)
P 		299
B 410.1
(-0.3)
P 		422.7
(-0.1)
P 		640
(3)
P 		341.9
(-1.3)
P 		264.3
(-2.4)
P 		224.6
(-3)
P 		415
C 432.4
(1.8)
P 		428.5
(1.7)
P 		458.9
(2.5)
P 		478.1
(3)
P 		253.2
(-2.7)
P 		240.9
(-3)
P 		237
avr 454.7
(1.4) 		441.1
(1.1) 		536.1
(3) 		400.8
(0.3) 		246.9
(-2.7) 		231
(-3) 		305
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 131
1 2 3 4 range
A 121
(3)
P 		56.7
(-0.7)
P 		64.1
(-0.2)
P 		15.6
(-3)
P 		105
B 100.9
(3)
P 		70.5
(0.9)
P 		62.8
(0.4)
P 		14.2
(-3)
A 		87
C 140.2
(3)
P 		83.3
(0.4)
P 		82.7
(0.4)
P 		9.5
(-3)
A 		131
avr 120.7
(3) 		70.2
(0.2) 		69.9
(0.2) 		13.1
(-3) 		108

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 96.2
(-0.8)
P 		168
(3)
P 		111
(-0)
P 		97
(-0.8)
P 		67.4
(-2.4)
P 		55.4
(-3)
P 		113
B 142.7
(1.9)
P 		140.7
(1.8)
P 		155.4
(3)
P 		90.6
(-2.4)
P 		107.4
(-1)
P 		83.8
(-3)
P 		72
C 160.3
(3)
P 		130.8
(0.8)
P 		116.8
(-0.3)
P 		96.1
(-1.8)
P 		81.9
(-2.9)
P 		80.7
(-3)
P 		80
avr 133.1
(1.9) 		146.5
(3) 		127.7
(1.5) 		94.6
(-1.3) 		85.6
(-2) 		73.3
(-3) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187249_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7867
Sequence Type Exemplar sequence
Representative Public ID K04H4.6B NCBI
Target Description K04H4.6B /REP_DB=WormBase Gene ID /WP=CE00251 /TR=SW:P34508 /GB=CAA81586.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066660(11), NM_066661(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066660 NCBI Caenorhabditis elegans Cell-death-Related Nuclease family member (crn-6) (crn-6) mRNA, complete cds. 11/11 A 
NM_066661 NCBI Caenorhabditis elegans Cell-death-Related Nuclease family member (crn-6) (crn-6) mRNA, complete cds. 11/11 A 
SNAP00000032563 ENSEMBL cdna:SNAP chromosome:CEL140:III:9367169:9369334:1 11/11 None
GENEFINDER00000032566 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9367169:9369334:1 11/11 None
K04H4.6a ENSEMBL cdna:known chromosome:CEL140:III:9367169:9369334:1 gene:K04H4.6 11/11 A 
K04H4.6b ENSEMBL cdna:known chromosome:CEL140:III:9367169:9370219:1 gene:K04H4.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9362341-9369137(+) 100.0 100.0
Public Domain and Genome References
Ensembl K04H4.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA81586 Hypothetical protein K04H4.6b [Caenorhabditis elegans] emb|CAD45582.1| Hypothetical protein K04H4.6b [Caenorhabditis elegans] ref|NP_499061.2| Cell-death-Related Nuclease family member (crn-6) [Caenorhabditis elegans] sp|P34508|CRN6_CAEEL Cell death-related nuclease 6 precursor 0.0
blast CAB76843 Hypothetical protein K04H4.6a [Caenorhabditis elegans] ref|NP_499062.1| Cell-death-Related Nuclease family member (crn-6) [Caenorhabditis elegans] gb|AAP57302.1| cell death-related nuclease 6 [Caenorhabditis elegans] 0.0
blast AAF43008 DNAse II homolog K04H4.6 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004947 EMBL-EBI Deoxyribonuclease II 4.7E-86
Sequence
Target Sequence 
>C. ELEGANS:187249_S_AT
gattcacagtattccaaagttcccgagtaaagatatgttccgattcccatcaaatgcgca
ttattatgggcaaatgggaatttgtatttcttacaatacggtttccttggcaactattgc
tcaacaactcttctactacaacacattcacttatcagttcaatttgccacagagttttgc
aaaccagtttcctgttttatctcaattgaaaaataaggaatataacaagagtccaccact
cacttccacgaaggttttgaaatcacttggtggccaacacttccgacatttcgcgaaaac
cggtgaatggggaaaagatctctacagcgattttgtcggtcctacattgaagtcttcgat
caaagttgaaacatggaatcatcagagcggagatgagtataatctcccatcagtttgtga
tcccaatcatgttcagtcgacaatgagtgcaaaatacattcgtcttccatatgcaatcga
ttactccagctatga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCACAGTATTCCAAAGTTCCCG 257 429 972 Antisense
TTCCGATTCCCATCAAATGCGCATT 220 699 1009 Antisense
TACGGTTTCCTTGGCAACTATTGCT 150 647 1068 Antisense
ATTGCTCAACAACTCTTCTACTACA 170 7 1087 Antisense
ACCAGTTTCCTGTTTTATCTCAATT 208 87 1154 Antisense
CACCACTCACTTCCACGAAGGTTTT 102 203 1205 Antisense
CCAACACTTCCGACATTTCGCGAAA 506 275 1245 Antisense
ACAGCGATTTTGTCGGTCCTACATT 386 109 1295 Antisense
GAGTATAATCTCCCATCAGTTTGTG 381 397 1366 Antisense
GTGATCCCAATCATGTTCAGTCGAC 301 483 1388 Antisense
ATGCAATCGATTACTCCAGCTATGA 386 43 1442 Antisense
Sequence Source Affymetrix Proprietary Database