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[187246_s_at]

Description K07E8.7 /REP_DB=WormBase Gene ID /WP=CE11898 /TR=SW:O16686 /GB=AAB66153.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 98      4grp_highest_8_8 > 31      4grp_high_8_8 > 20      6grp_highest_nonorm > 90      6grp_total > 98      4grp_total > 20      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 141
1 2 3 4 range
A 165.2
(-3)
P 		200.7
(-1.2)
P 		177.2
(-2.4)
P 		285.7
(3)
P 		121
B 206.1
(2.5)
P 		172.9
(-0.5)
P 		145
(-3)
P 		211.9
(3)
P 		67
C 217.4
(3)
P 		213.8
(2.4)
P 		207.9
(1.4)
P 		182
(-3)
P 		35
avr 196.2
(-0.7) 		195.8
(-0.7) 		176.7
(-3) 		226.5
(3) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 122.3
(-1.9)
P 		106.8
(-3)
P 		133.8
(-1.1)
P 		129.6
(-1.4)
P 		193.3
(3)
P 		187.2
(2.6)
P 		87
B 144.1
(-2.4)
P 		135.5
(-3)
P 		187.3
(0.4)
P 		156.5
(-1.6)
P 		221.6
(2.7)
P 		226.9
(3)
P 		91
C 101.4
(-2.5)
P 		140.3
(0.2)
P 		94.3
(-3)
P 		124
(-1)
P 		160.9
(1.6)
P 		181.3
(3)
P 		87
avr 122.6
(-3) 		127.5
(-2.6) 		138.5
(-1.7) 		136.7
(-1.9) 		191.9
(2.5) 		198.5
(3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 60.2
(3)
P 		43.6
(-2.4)
P 		46.3
(-1.5)
P 		41.8
(-3)
P 		18
B 65.7
(3)
P 		42.7
(-1.3)
P 		33.6
(-3)
P 		45.2
(-0.8)
P 		32
C 72
(2.9)
P 		61.5
(1.4)
P 		72.5
(3)
P 		30.7
(-3)
P 		42
avr 66
(3) 		49.3
(-0.7) 		50.8
(-0.4) 		39.2
(-3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 22.5
(-3)
P 		38
(-0.4)
P 		29.1
(-1.9)
P 		32.9
(-1.3)
P 		58.3
(3)
P 		45.6
(0.9)
P 		36
B 50.2
(-1.9)
P 		45.1
(-2.5)
P 		45.5
(-2.5)
P 		41.4
(-3)
P 		90
(3)
P 		84.6
(2.3)
P 		49
C 37.6
(-0.8)
P 		42.8
(0.1)
P 		24
(-3)
P 		24.9
(-2.9)
P 		52.1
(1.6)
P 		60.7
(3)
P 		37
avr 36.8
(-2.3) 		42
(-1.4) 		32.9
(-3) 		33.1
(-3) 		66.8
(3) 		63.6
(2.4) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187246_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5538
Sequence Type Exemplar sequence
Representative Public ID K07E8.7 NCBI
Target Description K07E8.7 /REP_DB=WormBase Gene ID /WP=CE11898 /TR=SW:O16686 /GB=AAB66153.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061377(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061377 NCBI Caenorhabditis elegans K07E8.7 (K07E8.7) mRNA, complete cds. 11/11 None
SNAP00000018449 ENSEMBL cdna:SNAP chromosome:CEL140:II:641071:644301:-1 11/11 None
GENEFINDER00000018465 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:641071:643607:-1 10/11 None
K07E8.7 ENSEMBL cdna:known chromosome:CEL140:II:641071:650373:-1 gene:K07E8.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:641070-650357(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K07E8.7
Entrez Gene 173456 Entrez gene
SwissProt O16686 EMBL-EBI
WormBase CE11898 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1585158_AT similar to RNA pseudouridylate synthase domain containing 2 cfa
CANINE_2:CFA.4769.1.A1_AT similar to RNA pseudouridylate synthase domain containing 2 cfa
CANINE_2:CFAAFFX.14474.1.S1_S_AT similar to RNA pseudouridylate synthase domain containing 2 cfa
DROSOPHILA_2:1637054_S_AT dm
DROSGENOME1:146164_AT dm
DROSGENOME1:146163_AT dm
HUGENEFL:U67934_AT RNA pseudouridylate synthase domain containing 2 hs
U133_X3P:HS.173311.0.S1_3P_AT RNA pseudouridylate synthase domain containing 2 hs
HG-U133A_2:221940_AT RNA pseudouridylate synthase domain containing 2 hs
HG-U133_PLUS_2:221940_AT RNA pseudouridylate synthase domain containing 2 hs
HG-U133A:221940_AT RNA pseudouridylate synthase domain containing 2 hs
HG-U95AV2:35075_AT RNA pseudouridylate synthase domain containing 2 hs
HG-U95E:78257_AT RNA pseudouridylate synthase domain containing 2 hs
HG-U95E:84757_AT RNA pseudouridylate synthase domain containing 2 hs
MOE430B:1453138_AT RNA pseudouridylate synthase domain containing 2 mm
MOUSE430_2:1453138_AT RNA pseudouridylate synthase domain containing 2 mm
MOE430B:1429442_AT RNA pseudouridylate synthase domain containing 2 mm
MOUSE430_2:1429442_AT RNA pseudouridylate synthase domain containing 2 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6396 RNA processing inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
4730 pseudouridylate synthase activity inferred from electronic annotation QuickGO AmiGO
9982 pseudouridine synthase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB66153 Hypothetical protein K07E8.7 [Caenorhabditis elegans] ref|NP_493778.1| K07E8.7 [Caenorhabditis elegans] sp|O16686|YK27_CAEEL Hypothetical protein K07E8.7 in chromosome IV 0.0
blast CAE72389 Hypothetical protein CBG19546 [Caenorhabditis briggsae] 1.0E-139
blast CAE72389 Hypothetical protein CBG19546 [Caenorhabditis briggsae] 7.0E-92
blast CAE72389 Hypothetical protein CBG19546 [Caenorhabditis briggsae] 8.0E-89
blast XP_624656 PREDICTED: similar to ENSANGP00000004675 [Apis mellifera] 5.0E-69
Protein Domains
Database ID Description E-Value
Pfam IPR006145 EMBL-EBI Pseudouridine synthase 6.4E-58
Pfam IPR006145 EMBL-EBI Pseudouridine synthase 5.2E-43
Pfam IPR006145 EMBL-EBI Pseudouridine synthase 6.4E-58
Sequence
Target Sequence 
>C. ELEGANS:187246_S_AT
agagcaagtctgtgagcagcccatcggcccgctggtcatctcaatgggaatccaatgtgt
tcggccggatggcaaggatgccaagtcgagattccgaaaactttggtcagatggaacaca
atcggtggttcaggtgcacattgagactggaagaactcatcagattcgggtccactcgca
gtttttaggccacccaatcgccggggaccaaatctacaactcggcggtctggggacccac
aaaaggaaaaaatgcagattaccagaagagttttgatgagctctgcgaagacgtgcggaa
cactcataaatgcgagaattggcacgagaagccgaatccagaattcgagcaacgaatgga
gcacctggcagccgacacaacgccaattacccctgaagctccgagcctgacactggagca
gcggccagaattcgatgagatttgtcagaaatgcaatgtggagtcgaagaaagtgcccga
gaatcacttccagttgtacctgcactgcttgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGCAAGTCTGTGAGCAGCCCATC 488 69 738 Antisense
GCCCGCTGGTCATCTCAATGGGAAT 244 287 764 Antisense
ATGGGAATCCAATGTGTTCGGCCGG 203 25 781 Antisense
AGAACTCATCAGATTCGGGTCCACT 459 73 889 Antisense
ATCGCCGGGGACCAAATCTACAACT 33 37 934 Antisense
TGAGCTCTGCGAAGACGTGCGGAAC 98 569 1014 Antisense
AGCAACGAATGGAGCACCTGGCAGC 72 77 1085 Antisense
TCCGAGCCTGACACTGGAGCAGCGG 99 595 1137 Antisense
GGAGCAGCGGCCAGAATTCGATGAG 707 519 1152 Antisense
TGCCCGAGAATCACTTCCAGTTGTA 634 583 1211 Antisense
TCCAGTTGTACCTGCACTGCTTGAA 140 591 1226 Antisense
Sequence Source Affymetrix Proprietary Database