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[187244_at]

Description B0228.1 /REP_DB=WormBase Gene ID /WP=CE01742 /TR=SW:Q09220 /GB=AAC38805.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 55      6grp_high1_10_10 > 44      4grp_highest_8_8 > 07      4grp_high_8_8 > 21      6grp_highest_nonorm > 74      6grp_total > 55      4grp_total > 18      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 766
1 2 3 4 range
A 670.9
(-2.2)
P 		585.9
(-3)
P 		588.4
(-3)
P 		1227
(3)
P 		641
B 581.7
(-3)
P 		827.3
(-0.9)
P 		646.4
(-2.5)
P 		1292.5
(3)
P 		711
C 672.4
(-2.8)
P 		1051.2
(0.4)
P 		650.8
(-3)
P 		1348
(3)
P 		697
avr 641.7
(-2.9) 		821.5
(-1.2) 		628.5
(-3) 		1289.2
(3) 		661

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 407
1 2 3 4 5 6 range
A 621
(-2.7)
P 		783.9
(0.4)
P 		923.2
(3)
P 		702
(-1.2)
P 		606.3
(-3)
P 		825.7
(1.2)
P 		317
B 587.8
(-2.9)
P 		873.6
(3)
P 		583.3
(-3)
P 		621.6
(-2.2)
P 		671.3
(-1.2)
P 		756.7
(0.6)
P 		290
C 515.9
(-3)
P 		890.4
(3)
P 		763.1
(1)
P 		684.3
(-0.3)
P 		565.2
(-2.2)
P 		874.6
(2.7)
P 		375
avr 574.9
(-3) 		849.3
(3) 		756.5
(1) 		669.3
(-0.9) 		614.3
(-2.1) 		819
(2.3) 		274
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 175
1 2 3 4 range
A 244.3
(3)
P 		127.2
(-3)
P 		153.8
(-1.6)
P 		179.6
(-0.3)
P 		117
B 185.5
(-1.3)
P 		204.3
(-0.4)
P 		149.8
(-3)
P 		275.4
(3)
P 		126
C 222.6
(-3)
P 		302.2
(3)
P 		226.8
(-2.7)
P 		227.3
(-2.6)
P 		80
avr 217.5
(1.8) 		211.2
(1.1) 		176.8
(-3) 		227.4
(3) 		51

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 178
1 2 3 4 5 6 range
A 114.5
(-3)
P 		278.8
(3)
P 		201.1
(0.2)
P 		178
(-0.7)
P 		182.9
(-0.5)
P 		201
(0.2)
P 		164
B 204.5
(-0.5)
P 		290.8
(3)
P 		141.7
(-3)
P 		164.7
(-2.1)
P 		272.7
(2.3)
P 		282.2
(2.7)
P 		149
C 191.2
(-0.9)
P 		271.7
(2.2)
P 		194.2
(-0.8)
P 		137.6
(-3)
P 		182.9
(-1.2)
P 		292.8
(3)
P 		155
avr 170.1
(-2.5) 		280.4
(3) 		179
(-2.1) 		160.1
(-3) 		212.8
(-0.4) 		258.7
(1.9) 		120
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187244_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5517
Sequence Type Exemplar sequence
Representative Public ID B0228.1 NCBI
Target Description B0228.1 /REP_DB=WormBase Gene ID /WP=CE01742 /TR=SW:Q09220 /GB=AAC38805.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063227(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063227 NCBI Caenorhabditis elegans B0228.1 (B0228.1) mRNA, complete cds. 11/11 None
SNAP00000020606 ENSEMBL cdna:SNAP chromosome:CEL140:II:7755929:7756767:1 11/11 None
GENEFINDER00000020739 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7756013:7756767:1 11/11 None
B0228.1 ENSEMBL cdna:known chromosome:CEL140:II:7756013:7756829:1 gene:B0228.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7756009-7756764(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0228.1
Entrez Gene 181861 Entrez gene
SwissProt Q09220 EMBL-EBI
WormBase CE01742 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC38805 Hypothetical protein B0228.1 [Caenorhabditis elegans] ref|NP_495628.1| B0228.1 [Caenorhabditis elegans] sp|Q09220|YP71_CAEEL Hypothetical protein B0228.1 in chromosome II 1.0E-105
blast CAE67468 Hypothetical protein CBG12970 [Caenorhabditis briggsae] 2.0E-91
blast CAE67468 Hypothetical protein CBG12970 [Caenorhabditis briggsae] 2.0E-84
Sequence
Target Sequence 
>C. ELEGANS:187244_AT
gaaggttcctatttctcatagaccaagaggtttcacagcaggtagcgattccaaaaagtt
ggctgtacagaggttcaaacttctcatctctgatttcttgtcagatgcgcaactgtccaa
gagtattgatgtagctgctgttggaatgcacaaaggaaagtcaatggatgatattttaga
tgatgtttacactcgtctccgcagaacattatccacaaaacaaataagtgagttagcaaa
agctcaaaaaggactagtcgaagatcttgatgaaaaatcagcaaagttagtgaaggcaag
agttaaacgaatgattgtctactcatttgatccggctgctgaacaaattcataaatatgc
aacacgtccttcaatggcttttgctctaattgctgaaacaatcaatgaaagatttgttgg
atcagtcaaggatttgattcgtgacgtactgactccaaaggaatatgatattttccgtaa
acactatcacccgcatatcttcaaattggac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGGTTCCTATTTCTCATAGACCA 119 335 39 Antisense
GGTTTCACAGCAGGTAGCGATTCCA 244 505 67 Antisense
AAACTTCTCATCTCTGATTTCTTGT 102 129 115 Antisense
TAGATGATGTTTACACTCGTCTCCG 496 653 215 Antisense
TCGTCTCCGCAGAACATTATCCACA 105 605 231 Antisense
GATTGTCTACTCATTTGATCCGGCT 403 435 351 Antisense
GCAACACGTCCTTCAATGGCTTTTG 473 319 397 Antisense
AATGGCTTTTGCTCTAATTGCTGAA 591 161 411 Antisense
GATTCGTGACGTACTGACTCCAAAG 441 431 474 Antisense
TATTTTCCGTAAACACTATCACCCG 294 663 507 Antisense
TCACCCGCATATCTTCAAATTGGAC 125 625 525 Antisense
Sequence Source Affymetrix Proprietary Database