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[187243_at]

Description B0523.3 /REP_DB=WormBase Gene ID /WP=CE20462 /TR=SW:P34266 /GB=AAB37089.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 96      4grp_high_8_8 > 21      6grp_total > 87      4grp_total > 18      6grp_cgc > 58      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 85
1 2 3 4 range
A 34.7
(-3)
A 		77.1
(1.3)
P 		40.9
(-2.4)
A 		93.2
(3)
M 		59
B 53.1
(-2.2)
P 		63.5
(-1.4)
A 		42.8
(-3)
A 		119.3
(3)
P 		77
C 48.2
(-3)
P 		64.1
(-0.9)
M 		54.1
(-2.2)
P 		93.2
(3)
P 		45
avr 45.3
(-3) 		68.2
(-0.6) 		45.9
(-2.9) 		101.9
(3) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 253
1 2 3 4 5 6 range
A 46.6
(-1.8)
A 		14.9
(-3)
A 		92.6
(0.1)
P 		81.2
(-0.4)
P 		112.1
(0.8)
P 		167.7
(3)
P 		153
B 44
(-2.9)
P 		40.2
(-3)
P 		48.7
(-2.8)
P 		66.7
(-2.3)
P 		173.4
(0.5)
P 		268.1
(3)
P 		228
C 27.8
(-3)
P 		40.7
(-2.7)
A 		70.7
(-1.9)
P 		71.5
(-1.9)
P 		82.2
(-1.6)
P 		262.5
(3)
P 		235
avr 39.5
(-2.8) 		31.9
(-3) 		70.7
(-1.8) 		73.1
(-1.8) 		122.6
(-0.3) 		232.8
(3) 		201
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 12.6
(-1.1)
A 		16.7
(3)
P 		10.7
(-3)
A 		13.6
(-0.1)
M 		6
B 16.9
(-0.3)
P 		15.7
(-0.8)
A 		9.9
(-3)
A 		25.4
(3)
P 		16
C 15.9
(-2.6)
P 		18.4
(2.2)
M 		18.8
(3)
P 		15.7
(-3)
P 		3
avr 15.1
(-0.6) 		16.9
(1.5) 		13.1
(-3) 		18.2
(3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 8.6
(-2.4)
A 		5.3
(-3)
A 		20.2
(-0.5)
P 		20.6
(-0.4)
P 		33.8
(1.8)
P 		40.8
(3)
P 		36
B 15.3
(-2.8)
P 		13.4
(-2.9)
P 		11.8
(-3)
P 		17.7
(-2.6)
P 		70.4
(1)
P 		100
(3)
P 		88
C 10.3
(-3)
P 		12.4
(-2.8)
A 		18
(-2.4)
P 		14.4
(-2.7)
P 		26.6
(-1.7)
P 		87.9
(3)
P 		78
avr 11.4
(-2.9) 		10.4
(-3) 		16.7
(-2.4) 		17.6
(-2.3) 		43.6
(0) 		76.2
(3) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187243_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7258
Sequence Type Exemplar sequence
Representative Public ID B0523.3 NCBI
Target Description B0523.3 /REP_DB=WormBase Gene ID /WP=CE20462 /TR=SW:P34266 /GB=AAB37089.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066510(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066510 NCBI Caenorhabditis elegans P GranuLe abnormality family member (pgl-2) (pgl-2) mRNA, complete cds. 11/11 None
SNAP00000022048 ENSEMBL cdna:SNAP chromosome:CEL140:III:8670558:8675296:-1 11/11 None
B0523.3 ENSEMBL cdna:known chromosome:CEL140:III:8670451:8674953:-1 gene:B0523.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000022052 8/11 Cross Hyb Matching Probes None
GENEFINDER00000022053 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8670552-8674944(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol pgl-2
Ensembl B0523.3
Entrez Gene 182022 Entrez gene
SwissProt P34266 EMBL-EBI
WormBase CE20462 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB37089 P granule abnormality protein 2 [Caenorhabditis elegans] ref|NP_498911.1| P GranuLe abnormality family member (pgl-2) [Caenorhabditis elegans] dbj|BAC87887.1| PGL-2 [Caenorhabditis elegans] sp|P34266|PGL2_CAEEL P granule abnormality protein 0.0
blast S44733 b0523.4 protein - Caenorhabditis elegans 0.0
blast H88535 protein B0523.4 [imported] - Caenorhabditis elegans 1.0E-179
blast H88535 protein B0523.4 [imported] - Caenorhabditis elegans 1.0E-154
Sequence
Target Sequence 
>C. ELEGANS:187243_AT
ggcaccgcaaagtttcgcaaaggctatcaaaaatgtttgggtgagacatgcaattattgg
gatcctcgactcactgaagttctcactcaacaaggatgtttttaatcaattagtgccgtt
gattcctgggagtcaagtttcgaacgatgtgatttctggaaaagttgaaaatgctatttc
atgcgcaatttggatcgtgaaaacatttggaaccgaaggctctagagactcaatcgaaaa
acttccatggttcgatagttcggtggaattgacagaattgataaattccattgaaattgc
tgtaacttcagtggaagttgaagaccagacagtcaacttcagaaaaatcccacaaaaaga
ttggttggatggaaatttgcagaaagcattaaaaacggagccaacttctgaaaaattcgg
aatttcgaattcactcaaaattaaggacgaaaatcaaagaaatcagattcggggattcaa
agataattccaaggaaccaaatgccaaagtttctgcaagatttggctcgaaattccaaag
agtggattctagtaccaagagacgtcttttcggtggagtagga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCACCGCAAAGTTTCGCAAAGGCT 424 535 993 Antisense
ATTATTGGGATCCTCGACTCACTGA 657 13 1045 Antisense
TTAGTGCCGTTGATTCCTGGGAGTC 419 651 1102 Antisense
TCCTGGGAGTCAAGTTTCGAACGAT 432 589 1116 Antisense
GCTATTTCATGCGCAATTTGGATCG 429 303 1165 Antisense
GGAACCGAAGGCTCTAGAGACTCAA 125 531 1201 Antisense
ACTTCCATGGTTCGATAGTTCGGTG 650 101 1233 Antisense
AGGAACCAAATGCCAAAGTTTCTGC 33 53 1484 Antisense
AAGTTTCTGCAAGATTTGGCTCGAA 430 157 1499 Antisense
GGATTCTAGTACCAAGAGACGTCTT 119 509 1536 Antisense
GAGACGTCTTTTCGGTGGAGTAGGA 118 391 1551 Antisense
Sequence Source Affymetrix Proprietary Database