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[187238_at]

Description F52B11.2 /REP_DB=WormBase Gene ID /WP=CE18722 /TR=SW:Q9XUE6 /GB=CAB05198.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 23      4grp_high_8_8 > 55      6grp_highest_nonorm > 41      6grp_total > 13      4grp_total > 57      6grp_high1_sub > 02      6grp_total_sub > Decrease      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1018
1 2 3 4 range
A 411.8
(-2.3)
P 		1115.9
(1.8)
P 		1312.4
(3)
P 		294.9
(-3)
A 		1018
B 472.9
(-2.1)
P 		858.6
(0.4)
P 		1258
(3)
P 		327.8
(-3)
P 		930
C 438.2
(-2.8)
P 		994.8
(0.9)
P 		1311.8
(3)
P 		411.3
(-3)
P 		901
avr 441
(-2.4) 		989.8
(1.1) 		1294.1
(3) 		344.7
(-3) 		949

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 486
1 2 3 4 5 6 range
A 775.4
(3)
P 		721.2
(1.8)
P 		691.4
(1.1)
P 		688.7
(1)
P 		621.1
(-0.5)
P 		509.9
(-3)
P 		266
B 828.8
(1.3)
P 		949
(3)
P 		789.1
(0.8)
P 		731.3
(-0)
P 		664.2
(-1)
P 		517.9
(-3)
P 		431
C 727.9
(-0.3)
P 		862.4
(1.4)
P 		702.5
(-0.6)
P 		992.1
(3)
P 		585.5
(-2)
P 		506.2
(-3)
P 		486
avr 777.4
(1.8) 		844.2
(3) 		727.7
(0.9) 		804
(2.3) 		623.6
(-1) 		511.3
(-3) 		333
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 414
1 2 3 4 range
A 149.9
(-0.9)
P 		242.2
(1)
P 		343.1
(3)
P 		43.2
(-3)
A 		300
B 150.8
(-0.8)
P 		212
(0.8)
P 		291.5
(3)
P 		69.9
(-3)
P 		222
C 145.1
(-1.8)
P 		286
(0.4)
P 		457.2
(3)
P 		69.4
(-3)
P 		388
avr 148.6
(-1.3) 		246.7
(0.7) 		363.9
(3) 		60.8
(-3) 		303

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 192
1 2 3 4 5 6 range
A 142.9
(-2.1)
P 		256.5
(3)
P 		150.6
(-1.8)
P 		174.7
(-0.7)
P 		187.4
(-0.1)
P 		124.1
(-3)
P 		132
B 288.4
(1.7)
P 		315.9
(3)
P 		191.6
(-3)
P 		193.7
(-2.9)
P 		269.9
(0.8)
P 		193.1
(-2.9)
P 		124
C 269.8
(3)
P 		263.1
(2.6)
P 		178.8
(-2.4)
P 		199.5
(-1.2)
P 		189.5
(-1.8)
P 		169.4
(-3)
P 		100
avr 233.7
(0.7) 		278.5
(3) 		173.7
(-2.4) 		189.3
(-1.6) 		215.6
(-0.2) 		162.2
(-3) 		116
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187238_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8574
Sequence Type Exemplar sequence
Representative Public ID F52B11.2 NCBI
Target Description F52B11.2 /REP_DB=WormBase Gene ID /WP=CE18722 /TR=SW:Q9XUE6 /GB=CAB05198.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070297(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070297 NCBI Caenorhabditis elegans F52B11.2 (F52B11.2) mRNA, complete cds. 11/11 None
SNAP00000035580 ENSEMBL cdna:SNAP chromosome:CEL140:IV:14083537:14084510:1 11/11 None
GENEFINDER00000035591 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:14083537:14084510:1 11/11 None
F52B11.2 ENSEMBL cdna:known chromosome:CEL140:IV:14083773:14084510:1 gene:F52B11.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:14083778-14084516(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F52B11.2
Entrez Gene 178364 Entrez gene
WormBase CE36163 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:14061_AT eukaryotic phosphomannomutase family protein at
ATH1-121501:266928_AT eukaryotic phosphomannomutase family protein at
CANINE:1604745_AT similar to Phosphomannomutase 2 (PMM 2) cfa
CANINE_2:CFAAFFX.29042.1.S1_AT similar to Phosphomannomutase 2 (PMM 2) cfa
DROSGENOME1:148668_AT dm
DROSOPHILA_2:1636257_AT dm
CHICKEN:GGA.13436.1.S1_S_AT phosphomannomutase 2 gga
HG-U133_PLUS_2:203201_AT phosphomannomutase 2 hs
HG-FOCUS:203201_AT phosphomannomutase 2 hs
HG-U133A:203201_AT phosphomannomutase 2 hs
HG-U133A_2:203201_AT phosphomannomutase 2 hs
HU35KSUBA:U85773_AT phosphomannomutase 2 hs
U133_X3P:G4557838_3P_AT phosphomannomutase 2 hs
HG-U95AV2:32028_AT phosphomannomutase 2 hs
HG-U95D:81800_R_AT Phosphomannomutase 2 hs
MOE430A:1431938_A_AT phosphomannomutase 2 mm
MOUSE430_2:1431938_A_AT phosphomannomutase 2 mm
MOUSE430A_2:1431938_A_AT phosphomannomutase 2 mm
MG-U74AV2:101949_AT phosphomannomutase 2 mm
MG-U74BV2:164722_F_AT phosphomannomutase 2 mm
MOE430A:1431464_A_AT phosphomannomutase 2 mm
MOUSE430A_2:1431464_A_AT phosphomannomutase 2 mm
MOUSE430_2:1431464_A_AT phosphomannomutase 2 mm
MU19KSUBC:TC35149_AT Phosphomannomutase 2 (Pmm2), mRNA mm
MU19KSUBC:TC36732_AT Phosphomannomutase 2 (Pmm2), mRNA mm
RG-U34C:RC_AI180239_AT phosphomannomutase 2 (predicted) rn
RAE230A:1371535_AT phosphomannomutase 2 (predicted) rn
RAT230_2:1371535_AT phosphomannomutase 2 (predicted) rn
RAE230B:1397458_AT phosphomannomutase 2 (predicted) rn
RAT230_2:1397458_AT phosphomannomutase 2 (predicted) rn
YEAST_2:1769378_AT Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
19307 mannose biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4615 phosphomannomutase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05198 Hypothetical protein F52B11.2 [Caenorhabditis elegans] ref|NP_502698.2| F52B11.2 [Caenorhabditis elegans] 1.0E-129
blast CAB05198 Hypothetical protein F52B11.2 [Caenorhabditis elegans] ref|NP_502698.2| F52B11.2 [Caenorhabditis elegans] 1.0E-128
blast Q9XUE6 Probable phosphomannomutase (PMM) 1.0E-125
blast CAE71768 Hypothetical protein CBG18762 [Caenorhabditis briggsae] 1.0E-102
Protein Domains
Database ID Description E-Value
Pfam IPR005002 EMBL-EBI Eukaryotic phosphomannomutase 1.0E-126
Pfam IPR005002 EMBL-EBI Eukaryotic phosphomannomutase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187238_AT
cctgttcgactacacattctcggagaacgggctctacggattcaagggcaccgaaccgta
tccagtacaatcaatccagaaagccatcggcgatgcgaagctccaagagctcatcaattt
tgctctccgctacatgtctgacattcaacttcctgtgaaacgtggaaatttcgtcgaatt
ccgaaacgggatgatcaatttgtcgcctattggacggagttgctcacaagaggaacgaat
gcaatttgtggagtttgataagaagcacggaattcgccaaaagttcaccgagcagctccg
cgagaagttcgggcaatacgggctccagtttgcaatcggaggacaaattagtgttgatgt
attccctactggatgggataagaccttttgtttgcaatatttagtgccagatttcgatac
aattcactttttcggagataaaaccgcgcctggcggaaacgatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCTGTTCGACTACACATTCTCGGAG 163 267 189 Antisense
CGGGCTCTACGGATTCAAGGGCACC 153 205 216 Antisense
CACCGAACCGTATCCAGTACAATCA 261 199 237 Antisense
ATCGGCGATGCGAAGCTCCAAGAGC 306 27 274 Antisense
ACGGGATGATCAATTTGTCGCCTAT 281 95 374 Antisense
GTCGCCTATTGGACGGAGTTGCTCA 230 475 390 Antisense
TCGCCAAAAGTTCACCGAGCAGCTC 385 599 462 Antisense
GCAGCTCCGCGAGAAGTTCGGGCAA 451 311 480 Antisense
CAATACGGGCTCCAGTTTGCAATCG 216 179 502 Antisense
AGTGTTGATGTATTCCCTACTGGAT 691 59 538 Antisense
AAACCGCGCCTGGCGGAAACGATCA 247 111 629 Antisense
Sequence Source Affymetrix Proprietary Database