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[187237_at]

Description ZK353.3 /REP_DB=WormBase Gene ID /WP=CE00387 /TR=SW:P34626 /GB=AAA28200.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 48      4grp_total > 28      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 43.7
(3)
A 		29.9
(0.9)
A 		4.6
(-3)
A 		25.7
(0.2)
A 		39
B 9.1
(-3)
A 		34.3
(3)
A 		27.6
(1.4)
A 		24.3
(0.6)
A 		25
C 7.5
(-1.7)
A 		3.5
(-3)
A 		5.4
(-2.4)
A 		22
(3)
A 		19
avr 20.1
(1) 		22.6
(2.3) 		12.5
(-3) 		24
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 4.3
(-3)
A 		25.5
(0.7)
A 		7.5
(-2.4)
A 		11.2
(-1.8)
A 		16.6
(-0.9)
A 		38.9
(3)
A 		35
B 42.9
(3)
A 		10.6
(-1.7)
A 		1.6
(-3)
A 		9.8
(-1.8)
A 		9.6
(-1.8)
A 		32.3
(1.5)
A 		41
C 43.2
(1.5)
A 		40.9
(1.2)
A 		8
(-3)
A 		8.5
(-2.9)
A 		11.5
(-2.6)
A 		55.4
(3)
P 		47
avr 30.1
(1) 		25.7
(0.3) 		5.7
(-3) 		9.8
(-2.3) 		12.6
(-1.9) 		42.2
(3) 		37
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 15.9
(3)
A 		6.5
(-0.8)
A 		1.2
(-3)
A 		3.8
(-1.9)
A 		15
B 2.9
(-3)
A 		8.5
(3)
A 		6.4
(0.8)
A 		5.2
(-0.5)
A 		6
C 2.5
(0.3)
A 		1
(-3)
A 		1.9
(-1)
A 		3.7
(3)
A 		3
avr 7.1
(3) 		5.3
(0.2) 		3.2
(-3) 		4.2
(-1.5) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 0.8
(-3)
A 		9.1
(2.7)
A 		1.6
(-2.4)
A 		2.8
(-1.6)
A 		5
(-0.1)
A 		9.5
(3)
A 		9
B 14.9
(3)
A 		3.5
(-1.7)
A 		0.4
(-3)
A 		2.6
(-2.1)
A 		3.9
(-1.6)
A 		12
(1.8)
A 		15
C 16
(2.1)
A 		12.5
(0.9)
A 		2
(-2.9)
A 		1.7
(-3)
A 		3.7
(-2.3)
A 		18.5
(3)
P 		17
avr 10.6
(1.7) 		8.4
(0.6) 		1.3
(-3) 		2.4
(-2.5) 		4.2
(-1.6) 		13.3
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187237_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7973
Sequence Type Exemplar sequence
Representative Public ID ZK353.3 NCBI
Target Description ZK353.3 /REP_DB=WormBase Gene ID /WP=CE00387 /TR=SW:P34626 /GB=AAA28200.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066451(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066451 NCBI Caenorhabditis elegans ZK353.3 (ZK353.3) mRNA, complete cds. 11/11 None
SNAP00000044093 ENSEMBL cdna:SNAP chromosome:CEL140:III:8395972:8396613:1 11/11 None
GENEFINDER00000044101 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8395972:8396613:1 11/11 None
ZK353.3 ENSEMBL cdna:known chromosome:CEL140:III:8395972:8396613:1 gene:ZK353.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8395967-8396609(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK353.3
Entrez Gene 191287 Entrez gene
SwissProt P34626 EMBL-EBI
WormBase CE00387 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA28200 Hypothetical protein ZK353.3 [Caenorhabditis elegans] ref|NP_498852.1| ZK353.3 [Caenorhabditis elegans] sp|P34626|YOJ3_CAEEL Hypothetical protein ZK353.3 in chromosome III 3.0E-83
Sequence
Target Sequence 
>C. ELEGANS:187237_AT
taagtcttgtcgcaccgactgtttccagtaccataagttcccggcttatgattggccccg
aaaataatgaaaaggatgcaaaaaattctgacagaacaaaatatgataatttggcaatgt
tgggacgttctcctgaacagaaagaacatgtaaatccatttgaaaaggctcccgtcgctc
gtcctactagaaatacggtggattctcaaatgatgcttggtcctcctaagaaggcaaacg
accaaagtgcttctgattgttcaatttatgataatctcgtagttccaatgtcagaattgg
gcccaactcctggaaataagccatctccaaacccaattgaaggagctcctatgattcgtc
caccccaagatactgtctcgagagctccggataattcaatttatgacactcttcagatga
caccgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAGTCTTGTCGCACCGACTGTTTC 196 635 26 Antisense
TTTCCAGTACCATAAGTTCCCGGCT 571 667 47 Antisense
GCAATGTTGGGACGTTCTCCTGAAC 47 323 139 Antisense
CGTCGCTCGTCCTACTAGAAATACG 513 245 198 Antisense
AATGATGCTTGGTCCTCCTAAGAAG 710 161 234 Antisense
ACGACCAAAGTGCTTCTGATTGTTC 386 91 263 Antisense
GATAATCTCGTAGTTCCAATGTCAG 224 423 295 Antisense
GAATTGGGCCCAACTCCTGGAAATA 410 321 319 Antisense
GAAGGAGCTCCTATGATTCGTCCAC 101 335 364 Antisense
ATACTGTCTCGAGAGCTCCGGATAA 487 23 395 Antisense
ATGACACTCTTCAGATGACACCGGA 80 45 428 Antisense
Sequence Source Affymetrix Proprietary Database