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[187233_s_at]

Description Y77E11A.11 /REP_DB=WormBase Gene ID /WP=CE25612 /TR=Q9N4B1 /GB=AAF36080.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 31      4grp_highest_8_8 > 19      4grp_high_8_8 > 63      6grp_highest_nonorm > 51      6grp_total > 34      4grp_total > 40      6grp_highest_sub > 01_02_03      6grp_cgc > 80      6grp_high1_sub > 03      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 398.7
(-3)
P 		464.3
(3)
P 		452.5
(1.9)
P 		419.9
(-1.1)
P 		66
B 369
(-3)
P 		387.5
(-2.3)
P 		535.4
(3)
P 		368.3
(-3)
P 		167
C 367.1
(2.1)
P 		315
(-3)
P 		343.8
(-0.2)
P 		376.6
(3)
P 		62
avr 378.3
(-3) 		388.9
(-2) 		443.9
(3) 		388.3
(-2.1) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 486
1 2 3 4 5 6 range
A 453.7
(-3)
P 		547.9
(0.4)
P 		621.3
(3)
P 		494.5
(-1.5)
P 		462.6
(-2.7)
P 		519.5
(-0.6)
P 		168
B 623.2
(0.4)
P 		655.1
(0.9)
P 		789.2
(3)
P 		425.1
(-2.8)
P 		413
(-3)
P 		487.6
(-1.8)
P 		376
C 712.8
(3)
P 		534.4
(0.4)
P 		517.2
(0.1)
P 		333
(-2.6)
P 		548.5
(0.6)
P 		303.1
(-3)
P 		410
avr 596.6
(1.8) 		579.1
(1.3) 		642.6
(3) 		417.5
(-3) 		474.7
(-1.5) 		436.7
(-2.5) 		225
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 145.2
(3)
P 		100.8
(-0.2)
P 		118.3
(1.1)
P 		61.5
(-3)
P 		84
B 117.7
(2.2)
P 		95.7
(-0.7)
P 		124.1
(3)
P 		78.5
(-3)
P 		46
C 121.5
(3)
P 		90.6
(-0.2)
P 		119.8
(2.8)
P 		63.5
(-3)
P 		58
avr 128.1
(3) 		95.7
(-0.2) 		120.7
(2.3) 		67.8
(-3) 		60

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 197
1 2 3 4 5 6 range
A 83.6
(-3)
P 		194.8
(3)
P 		135.3
(-0.2)
P 		125.4
(-0.7)
P 		139.6
(0)
P 		126.5
(-0.7)
P 		111
B 216.9
(2.9)
P 		218.1
(3)
P 		191.6
(1.5)
P 		112.6
(-3)
P 		167.8
(0.1)
P 		181.8
(0.9)
P 		106
C 264.2
(3)
P 		163.1
(-0.1)
P 		131.6
(-1)
P 		67
(-3)
P 		177.5
(0.4)
P 		101.5
(-2)
P 		197
avr 188.2
(2.7) 		192
(3) 		152.8
(0.4) 		101.7
(-3) 		161.6
(1) 		136.6
(-0.7) 		90
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187233_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10947
Sequence Type Exemplar sequence
Representative Public ID Y77E11A.11 NCBI
Target Description Y77E11A.11 /REP_DB=WormBase Gene ID /WP=CE25612 /TR=Q9N4B1 /GB=AAF36080.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067681(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067681 NCBI Caenorhabditis elegans CaLPain family member (clp-7) (clp-7) mRNA, complete cds. 11/11 A 
SNAP00000027309 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1431319:1438372:-1 11/11 None
GENEFINDER00000027324 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1427491:1440564:-1 11/11 None
Y77E11A.11.1 ENSEMBL cdna:known chromosome:CEL140:IV:1435474:1445992:-1 gene:Y77E11A.11 11/11 None
Y77E11A.11.2 ENSEMBL cdna:known chromosome:CEL140:IV:1435626:1446026:-1 gene:Y77E11A.11 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1436952-1445971(-) 98.29 99.44
Public Domain and Genome References
Gene Title
Gene Symbol clp-7
Ensembl Y77E11A.11
Entrez Gene 176953 Entrez gene
SwissProt Q9N4B1 EMBL-EBI
WormBase CE25612 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.25167.1.S1_AT similar to calpain 8 cfa
CANINE_2:CFAAFFX.25167.1.S1_S_AT similar to calpain 8 cfa
CANINE_2:CFAAFFX.25171.1.S1_AT similar to calpain 8 cfa
CHICKEN:GGA.6487.1.S1_AT similar to calpain 8 gga
CHICKEN:GGAAFFX.22908.2.S1_AT similar to calpain 8 gga
CHICKEN:GGAAFFX.22908.2.S1_S_AT similar to calpain 8 gga
CHICKEN:GGAAFFX.22908.3.S1_S_AT similar to calpain 8 gga
CHICKEN:GGAAFFX.5920.1.S1_S_AT similar to calpain 8 gga
MOE430A:1421634_AT calpain 8 mm
MOUSE430_2:1421634_AT calpain 8 mm
MOUSE430A_2:1421634_AT calpain 8 mm
MOUSE430_2:1425916_AT calpain 8 mm
MOE430A:1425916_AT calpain 8 mm
MOUSE430A_2:1425916_AT calpain 8 mm
MG-U74CV2:170523_I_AT calpain 8 mm
RAT230_2:1387991_AT calpain 8 rn
RAE230A:1387991_AT calpain 8 rn
RN-U34:D14478_S_AT calpain 8 rn
RG-U34A:D14478_S_AT calpain 8 rn
RN-U34:D14480_AT calpain 8 rn
RG-U34A:D14480_AT calpain 8 rn
RAE230A:1387292_S_AT calpain 8 rn
RAT230_2:1387292_S_AT calpain 8 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4197 cysteine-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4198 calpain activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF36080 Calpain family protein 7 [Caenorhabditis elegans] ref|NP_500082.1| CaLPain family member (clp-7) [Caenorhabditis elegans] 0.0
blast CAE68248 Hypothetical protein CBG13923 [Caenorhabditis briggsae] 0.0
blast AAF36079 Calpain family protein 6 [Caenorhabditis elegans] ref|NP_500081.1| CaLPain family member (clp-6) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.0E-126
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.7E-50
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 3.4E-57
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.0E-126
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.7E-50
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.6E-42
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.5E-69
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.0E-126
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.7E-50
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.6E-42
Pfam IPR008164 EMBL-EBI Repeat of unknown function XGLTT 6.8E-10
Pfam IPR008164 EMBL-EBI Repeat of unknown function XGLTT 3.9E-9
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000027324 3 7-26,36-58,457-479
Sequence
Target Sequence 
>C. ELEGANS:187233_S_AT
gacgattttatgaccaatttcacgcagatggaatgctgtaacttgtctgccgatgttatg
gatgagatttcggagatgactggtgtggaggtccacgacaagcaaaagcaccaatgggtc
gaaaaatcggaggacggagaatggaattcccgtcaaggaaccgccggtggatgtcagaat
aatgatacctattgtaataatccccaatatggtacatacttccaagtacccatggattct
gttgagcacgacgggaagtgtactgtaattggagcagtgctccagaaatatcgtcgagag
ctccggactaagggacttgacaatttgccaattggattttcagtctataaagctgatggc
tcgggacaggcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACGATTTTATGACCAATTTCACGC 631 377 1783 Antisense
GAATGCTGTAACTTGTCTGCCGATG 325 331 1813 Antisense
GAGATGACTGGTGTGGAGGTCCACG 99 397 1855 Antisense
GAGGTCCACGACAAGCAAAAGCACC 270 403 1870 Antisense
GAATGGAATTCCCGTCAAGGAACCG 179 333 1921 Antisense
AGGAACCGCCGGTGGATGTCAGAAT 617 53 1938 Antisense
GTACATACTTCCAAGTACCCATGGA 452 459 1994 Antisense
GTACCCATGGATTCTGTTGAGCACG 140 459 2008 Antisense
GAAATATCGTCGAGAGCTCCGGACT 623 361 2067 Antisense
GAGCTCCGGACTAAGGGACTTGACA 11 387 2080 Antisense
AAAGCTGATGGCTCGGGACAGGCGA 299 123 2131 Antisense
Sequence Source Affymetrix Proprietary Database