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[187220_at]

Description D1044.6 /REP_DB=WormBase Gene ID /WP=CE01209 /TR=SW:P41954 /GB=AAA50738.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 69      6grp_highest_nonorm > 91      6grp_total > 79      4grp_total > 31      6grp_highest_sub > Cu      6grp_high1_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 177.3
(3)
P 		80.5
(-2.8)
A 		103.2
(-1.4)
A 		77.2
(-3)
A 		100
B 143.8
(1.2)
P 		87.6
(-3)
A 		112.5
(-1.1)
P 		168.2
(3)
P 		81
C 176.9
(3)
P 		100
(-3)
A 		99.8
(-3)
P 		149.3
(0.9)
A 		77
avr 166
(3) 		89.4
(-3) 		105.2
(-1.8) 		131.6
(0.3) 		77

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 144
1 2 3 4 5 6 range
A 147.9
(-2.3)
P 		196.8
(-0.3)
P 		131.4
(-3)
P 		137
(-2.8)
P 		170.1
(-1.4)
P 		274.8
(3)
P 		143
B 182.3
(0.1)
P 		170.6
(-1.2)
P 		166
(-1.7)
P 		153.8
(-3)
P 		184.5
(0.3)
P 		209.3
(3)
P 		56
C 167
(-0.9)
P 		181.5
(-0)
P 		130.7
(-3)
P 		149.6
(-1.9)
P 		221.7
(2.3)
P 		232.9
(3)
P 		102
avr 165.7
(-1.6) 		183
(-0.5) 		142.7
(-3) 		146.8
(-2.7) 		192.1
(0.1) 		239
(3) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 64.6
(3)
P 		17.5
(-2.3)
A 		27
(-1.2)
A 		11.3
(-3)
A 		53
B 45.9
(3)
P 		21.6
(-3)
A 		26.1
(-1.9)
P 		35.9
(0.5)
P 		24
C 58.6
(3)
P 		28.7
(-2.4)
A 		34.8
(-1.3)
P 		25.2
(-3)
A 		33
avr 56.4
(3) 		22.6
(-3) 		29.3
(-1.8) 		24.1
(-2.7) 		34

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 27.3
(-3)
P 		70
(3)
P 		28.6
(-2.8)
P 		34.8
(-1.9)
P 		51.3
(0.4)
P 		66.9
(2.6)
P 		43
B 63.4
(0.7)
P 		56.8
(-0.4)
P 		40.3
(-3)
P 		40.7
(-2.9)
P 		75
(2.5)
P 		78
(3)
P 		38
C 61.9
(1)
P 		55.4
(0.2)
P 		33.3
(-2.6)
P 		30.1
(-3)
P 		71.7
(2.2)
P 		78
(3)
P 		48
avr 50.9
(-0.5) 		60.7
(1) 		34.1
(-3) 		35.2
(-2.8) 		66
(1.8) 		74.3
(3) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187220_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7408
Sequence Type Exemplar sequence
Representative Public ID D1044.6 NCBI
Target Description D1044.6 /REP_DB=WormBase Gene ID /WP=CE01209 /TR=SW:P41954 /GB=AAA50738.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065783(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065783 NCBI Caenorhabditis elegans D1044.6 (D1044.6) mRNA, complete cds. 11/11 None
SNAP00000008691 ENSEMBL cdna:SNAP chromosome:CEL140:III:5536157:5541366:-1 11/11 None
GENEFINDER00000008697 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5536157:5541366:-1 11/11 None
D1044.6 ENSEMBL cdna:known chromosome:CEL140:III:5535629:5540736:-1 gene:D1044.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5536155-5541064(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl D1044.6
Entrez Gene 175762 Entrez gene
SwissProt P41954 EMBL-EBI
WormBase CE32173 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA50738 Hypothetical protein D1044.6 [Caenorhabditis elegans] ref|NP_498184.2| D1044.6 [Caenorhabditis elegans] sp|P41954|YLK6_CAEEL Hypothetical protein D1044.6 in chromosome III 0.0
blast CAE56364 Hypothetical protein CBG24039 [Caenorhabditis briggsae] 1.0E-149
blast CAE56364 Hypothetical protein CBG24039 [Caenorhabditis briggsae] 1.0E-148
blast CAE56924 Hypothetical protein CBG24769 [Caenorhabditis briggsae] 1.0E-148
Protein Domains
Database ID Description E-Value
scop a.2.6.Effector domain of the protein kinase pkn/prk1 All alpha proteins; Long alpha-hairpin; Effector domain of the protein kinase pkn/prk1; Effector domain of the protein kinase pkn/prk1 0.560000002384186
scop a.2.6.Effector domain of the protein kinase pkn/prk1 All alpha proteins; Long alpha-hairpin; Effector domain of the protein kinase pkn/prk1; Effector domain of the protein kinase pkn/prk1 5.19999980926514
scop a.2.6.Effector domain of the protein kinase pkn/prk1 All alpha proteins; Long alpha-hairpin; Effector domain of the protein kinase pkn/prk1; Effector domain of the protein kinase pkn/prk1 5.09999990463257
Sequence
Target Sequence 
>C. ELEGANS:187220_AT
atttgcgtgagcttgtccgtgatgcacgtctcgacgcgttctcattcaaagaagaattca
aggatttcgaaggcggattcgaagattacccgggaacacgtgtcgaaccgtatgaaaatg
attggtactcccatggatacactctttattcgcatacacgtgatgctgcatacgctgatt
acatgcatgatgtatgtaaaccgtacgaggatatgctcccgccaactgaatatcacgaaa
ttttcaacgaatggtgctttacgccgatgctcgcctcgcttcattatcagttgaaaaaga
atgcatttgatgttctctcaccgcaagacgtgaaagatatggaagctcgtcatcgaaaat
acattggacttgtccagaaagagcgaaaattcagtttggagcaagcgctcgatgtgtgca
agttgaccgattacgataaacggcaatggaccattcttcacgatcagcttgtcactcatt
tcaaagacttcccttcaatttcgggcttggtgtacgacagaaatactaatctattagcga
acgtcaacagaaatttgaagcacctgttctcgacgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTTGCGTGAGCTTGTCCGTGATGC 306 17 2519 Antisense
GAAGATTACCCGGGAACACGTGTCG 607 335 2599 Antisense
GATTGGTACTCCCATGGATACACTC 390 435 2638 Antisense
GGATACACTCTTTATTCGCATACAC 456 511 2653 Antisense
AAACCGTACGAGGATATGCTCCCGC 126 125 2716 Antisense
ACGAATGGTGCTTTACGCCGATGCT 693 91 2765 Antisense
AGCGCTCGATGTGTGCAAGTTGACC 623 81 2922 Antisense
GACCATTCTTCACGATCAGCTTGTC 615 383 2967 Antisense
GATCAGCTTGTCACTCATTTCAAAG 151 421 2980 Antisense
AGACTTCCCTTCAATTTCGGGCTTG 284 69 3003 Antisense
ATTTGAAGCACCTGTTCTCGACGAT 110 19 3071 Antisense
Sequence Source Affymetrix Proprietary Database