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[187213_at]

Description EEED8.3 /REP_DB=WormBase Gene ID /WP=CE01887 /TR=SW:Q09294 /GB=AAC46763.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 87      4grp_total > 03      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 49.7
(-0.3)
A 		7.4
(-2.9)
A 		5.1
(-3)
A 		102.8
(3)
A 		98
B 34.9
(-3)
A 		51.1
(-1.3)
A 		36.1
(-2.9)
A 		91.9
(3)
P 		57
C 9.9
(-2.9)
A 		8.9
(-3)
A 		26.7
(-1.4)
A 		76.6
(3)
P 		68
avr 31.5
(-2.2) 		22.5
(-3) 		22.6
(-3) 		90.4
(3) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 934
1 2 3 4 5 6 range
A 29.2
(-2.8)
A 		4.1
(-3)
A 		10.7
(-2.9)
A 		41.6
(-2.7)
A 		399.6
(0.3)
P 		731
(3)
P 		727
B 51.8
(-2.7)
A 		6.9
(-3)
A 		49.1
(-2.7)
A 		43.2
(-2.7)
P 		449
(0.1)
P 		849.4
(3)
P 		843
C 43.4
(-2.8)
A 		7.4
(-3)
A 		9.5
(-3)
A 		22.2
(-2.9)
A 		351.7
(-0.8)
P 		937.7
(3)
P 		930
avr 41.5
(-2.7) 		6.1
(-3) 		23.1
(-2.9) 		35.7
(-2.8) 		400.1
(-0.2) 		839.4
(3) 		833
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 18.1
(3)
A 		1.6
(-2.9)
A 		1.3
(-3)
A 		15.1
(1.9)
A 		17
B 11.1
(-1.6)
A 		12.6
(-0.8)
A 		8.4
(-3)
A 		19.6
(3)
P 		11
C 3.3
(-2.5)
A 		2.5
(-3)
A 		9.3
(0.9)
A 		12.9
(3)
P 		10
avr 10.8
(0) 		5.6
(-3) 		6.3
(-2.6) 		15.9
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 315
1 2 3 4 5 6 range
A 5.4
(-2.9)
A 		1.5
(-3)
A 		2.3
(-3)
A 		10.5
(-2.7)
A 		120.6
(1)
P 		178
(3)
P 		177
B 18
(-2.7)
A 		2.3
(-3)
A 		11.9
(-2.8)
A 		11.4
(-2.8)
P 		182.4
(0.4)
P 		316.7
(3)
P 		314
C 16.1
(-2.7)
A 		2.2
(-3)
A 		2.4
(-3)
A 		4.5
(-3)
A 		113.8
(-0.9)
P 		313.9
(3)
P 		312
avr 13.2
(-2.7) 		2
(-3) 		5.5
(-2.9) 		8.8
(-2.8) 		138.9
(0.1) 		269.5
(3) 		268
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187213_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4657
Sequence Type Exemplar sequence
Representative Public ID EEED8.3 NCBI
Target Description EEED8.3 /REP_DB=WormBase Gene ID /WP=CE01887 /TR=SW:Q09294 /GB=AAC46763.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062624(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062624 NCBI Caenorhabditis elegans EEED8.3 (EEED8.3) mRNA, complete cds. 11/11 None
SNAP00000023177 ENSEMBL cdna:SNAP chromosome:CEL140:II:5414581:5415239:1 11/11 None
GENEFINDER00000023190 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5414581:5415239:1 11/11 None
EEED8.3 ENSEMBL cdna:known chromosome:CEL140:II:5414581:5415239:1 gene:EEED8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5414578-5415237(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl EEED8.3
Entrez Gene 184042 Entrez gene
SwissProt Q09294 EMBL-EBI
WormBase CE01887 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5488 binding inferred from electronic annotation QuickGO AmiGO
8289 lipid binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46763 Hypothetical protein EEED8.3 [Caenorhabditis elegans] ref|NP_495025.1| EEED8.3 [Caenorhabditis elegans] sp|Q09294|YQO3_CAEEL Hypothetical protein EEED8.3 in chromosome II 1.0E-100
blast CAE59098 Hypothetical protein CBG02391 [Caenorhabditis briggsae] 3.0E-37
Sequence
Target Sequence 
>C. ELEGANS:187213_AT
caaatcctcactgccatgatcgggaaatggaaacttgcatcctctgaaaatctccaagaa
tatttcactctcgaaaagttcccggagataacccaaatggcctgggagcacggaattact
tgttacaagatgaacggcaatcaacttcatgttcacaccgatttgctcggaaaatctctg
attcctacaattttcgagtttgataagccaattgccagggacgacaatgcggtttcaacg
catgcagaaggaaacatgatgagcaccatttgcaagaggattgctgacggatcgattgtt
tggaaagtggagagactcatcaaaaatggaaatcttgtggttttcaacagcaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAATCCTCACTGCCATGATCGGGA 190 193 202 Antisense
GAAACTTGCATCCTCTGAAAATCTC 223 357 231 Antisense
AATCTCCAAGAATATTTCACTCTCG 533 169 250 Antisense
TTTCACTCTCGAAAAGTTCCCGGAG 434 669 264 Antisense
TCCCGGAGATAACCCAAATGGCCTG 500 591 281 Antisense
GGGAGCACGGAATTACTTGTTACAA 513 493 305 Antisense
CAATCAACTTCATGTTCACACCGAT 531 181 339 Antisense
AATCTCTGATTCCTACAATTTTCGA 91 171 374 Antisense
GATAAGCCAATTGCCAGGGACGACA 607 419 403 Antisense
CAGGGACGACAATGCGGTTTCAACG 225 209 417 Antisense
AAATCTTGTGGTTTTCAACAGCAGA 359 117 531 Antisense
Sequence Source Affymetrix Proprietary Database