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[187206_at]

Description T20G5.10 /REP_DB=WormBase Gene ID /WP=CE00629 /TR=SW:Q22616 /GB=CAA83011.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 85      4grp_highest_8_8 > 63      4grp_high_8_8 > 19      6grp_highest_nonorm > 73      6grp_total > 74      4grp_total > 29      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 341.6
(-0.5)
P 		411.3
(3)
P 		293.2
(-3)
P 		379.8
(1.4)
P 		118
B 359.9
(1.3)
P 		301.2
(-3)
P 		368.3
(1.9)
P 		383.9
(3)
P 		83
C 421.6
(3)
P 		369.5
(0.4)
P 		301
(-3)
P 		382.9
(1.1)
P 		121
avr 374.4
(2.2) 		360.7
(0.9) 		320.8
(-3) 		382.2
(3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 541
1 2 3 4 5 6 range
A 329.9
(-3)
P 		458
(-0.2)
P 		603.7
(3)
P 		508.9
(0.9)
P 		494.6
(0.6)
P 		532.2
(1.4)
P 		274
B 354.1
(-3)
P 		384.4
(-2.4)
P 		655.1
(2.5)
P 		491.9
(-0.5)
P 		637.7
(2.2)
P 		684.3
(3)
P 		330
C 281.5
(-3)
P 		379.2
(-1.9)
P 		554
(0)
P 		568.3
(0.2)
P 		597.4
(0.5)
P 		822.3
(3)
P 		541
avr 321.8
(-3) 		407.2
(-1.6) 		604.3
(1.7) 		523
(0.4) 		576.6
(1.3) 		679.6
(3) 		358
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 84
1 2 3 4 range
A 124.4
(3)
P 		89.3
(-0.1)
P 		76.7
(-1.2)
P 		55.6
(-3)
P 		69
B 114.8
(3)
P 		74.4
(-3)
P 		85.3
(-1.4)
P 		81.8
(-1.9)
P 		40
C 139.5
(3)
P 		106.2
(0.3)
P 		104.9
(0.2)
P 		64.6
(-3)
P 		75
avr 126.2
(3) 		90
(-0.7) 		89
(-0.8) 		67.3
(-3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 215
1 2 3 4 5 6 range
A 60.8
(-3)
P 		162.9
(3)
P 		131.5
(1.2)
P 		129.1
(1)
P 		149.2
(2.2)
P 		129.6
(1)
P 		102
B 123.2
(-3)
P 		128
(-2.8)
P 		159.1
(-1.4)
P 		130.3
(-2.7)
P 		259.1
(3)
P 		255.2
(2.8)
P 		136
C 104.3
(-3)
P 		115.7
(-2.6)
P 		141
(-1.7)
P 		114.3
(-2.6)
P 		193.3
(0.1)
P 		275.3
(3)
P 		171
avr 96.1
(-3) 		135.5
(-1.1) 		143.9
(-0.7) 		124.6
(-1.6) 		200.5
(2.1) 		220
(3) 		124
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187206_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6426
Sequence Type Exemplar sequence
Representative Public ID T20G5.10 NCBI
Target Description T20G5.10 /REP_DB=WormBase Gene ID /WP=CE00629 /TR=SW:Q22616 /GB=CAA83011.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066861(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066861 NCBI Caenorhabditis elegans T20G5.10 (T20G5.10) mRNA, complete cds. 11/11 None
SNAP00000033305 ENSEMBL cdna:SNAP chromosome:CEL140:III:10211033:10211829:-1 11/11 None
GENEFINDER00000033345 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10211033:10211829:-1 11/11 None
T20G5.10 ENSEMBL cdna:known chromosome:CEL140:III:10211033:10211834:-1 gene:T20G5.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10211028-10211807(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl T20G5.10
Entrez Gene 176435 Entrez gene
SwissProt Q22616 EMBL-EBI
WormBase CE00629 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1591059_AT similar to general control of amino acid synthesis-like 1 cfa
CANINE_2:CFA.8257.1.A1_S_AT similar to general control of amino acid synthesis-like 1 cfa
CANINE_2:CFAAFFX.1061.1.S1_AT similar to general control of amino acid synthesis-like 1 cfa
DROSOPHILA_2:1630798_AT dm
DROSGENOME1:147209_AT dm
HG-U133_PLUS_2:202592_AT biogenesis of lysosome-related organelles complex-1, subunit 1 hs
HG-FOCUS:202592_AT biogenesis of lysosome-related organelles complex-1, subunit 1 hs
HG-U133A:202592_AT biogenesis of lysosome-related organelles complex-1, subunit 1 hs
HG-U133A_2:202592_AT biogenesis of lysosome-related organelles complex-1, subunit 1 hs
HU35KSUBC:RC_D53799_S_AT biogenesis of lysosome-related organelles complex-1, subunit 1 hs
HUGENEFL:S82447_S_AT biogenesis of lysosome-related organelles complex-1, subunit 1 hs
U133_X3P:G4503954_3P_AT biogenesis of lysosome-related organelles complex-1, subunit 1 hs
HG-U95AV2:39133_AT biogenesis of lysosome-related organelles complex-1, subunit 1 hs
MOUSE430A_2:1422614_S_AT biogenesis of lysosome-related organelles complex-1, subunit 1 mm
MOE430A:1422614_S_AT biogenesis of lysosome-related organelles complex-1, subunit 1 mm
MOUSE430_2:1422614_S_AT biogenesis of lysosome-related organelles complex-1, subunit 1 mm
MG-U74AV2:97917_AT biogenesis of lysosome-related organelles complex-1, subunit 1 mm
MG-U74BV2:164574_AT biogenesis of lysosome-related organelles complex-1, subunit 1 mm
MU19KSUBB:TC26284_AT Biogenesis of lysosome-related organelles complex-1, subunit 1, mRNA (cDNA clone MGC:35972 IMAGE:5359972) mm
RG-U34B:RC_AA997882_AT biogenesis of lysosome-related organelles complex-1, subunit 1 (predicted) rn
RAE230A:1372137_AT biogenesis of lysosome-related organelles complex-1, subunit 1 (predicted) rn
RAT230_2:1372137_AT biogenesis of lysosome-related organelles complex-1, subunit 1 (predicted) rn
Protein Similarities
Method ID Description E-Value
blast CAE64974 Hypothetical protein CBG09808 [Caenorhabditis briggsae] 4.0E-60
blast CAA83011 Hypothetical protein T20G5.10 [Caenorhabditis elegans] ref|NP_499262.1| T20G5.10 [Caenorhabditis elegans] sp|Q22616|YNXA_CAEEL Hypothetical protein T20G5.10 in chromosome III 9.0E-58
blast CAE64974 Hypothetical protein CBG09808 [Caenorhabditis briggsae] 8.0E-57
blast AAH82449 MGC84073 protein [Xenopus laevis] 6.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR009395 EMBL-EBI GCN5-like 1 1.4E-97
Pfam IPR009395 EMBL-EBI GCN5-like 1 1.4E-97
Sequence
Target Sequence 
>C. ELEGANS:187206_AT
tatcgtggcagctcaaaccttatctactgccgtcgtggatcatctgaatgcaaaagtcgc
tcaagcctacggaaaccaaaaacgcctggacgttgaagcgaaacgattcgaaaacaattc
agcagctcttgcgaaacaaactgaacaatggcttttcattaccgaaggcctaaattatgc
tctcaaggaaattggcgatgtcgaaaattggtcgaaaacgattgagaacgatatgaagat
tatcacggaaacattgcgcagagcctatgaagccaaaaaccctccactt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TATCGTGGCAGCTCAAACCTTATCT 327 653 81 Antisense
TACTGCCGTCGTGGATCATCTGAAT 437 647 105 Antisense
GAATGCAAAAGTCGCTCAAGCCTAC 181 331 126 Antisense
GTCGCTCAAGCCTACGGAAACCAAA 229 473 136 Antisense
AACGCCTGGACGTTGAAGCGAAACG 542 143 161 Antisense
AACAATTCAGCAGCTCTTGCGAAAC 190 133 193 Antisense
ATGGCTTTTCATTACCGAAGGCCTA 20 53 228 Antisense
CGAAGGCCTAAATTATGCTCTCAAG 125 253 243 Antisense
ATCACGGAAACATTGCGCAGAGCCT 83 29 322 Antisense
CATTGCGCAGAGCCTATGAAGCCAA 644 215 332 Antisense
CTATGAAGCCAAAAACCCTCCACTT 165 213 345 Antisense
Sequence Source Affymetrix Proprietary Database