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[187200_s_at]

Description E04A4.5 /REP_DB=WormBase Gene ID /WP=CE16966 /TR=SW:O44477 /GB=AAB92039.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 32      6grp_high1_10_10 > 23      4grp_highest_8_8 > 08      4grp_high_8_8 > 44      6grp_highest_nonorm > 31      6grp_total > 13      4grp_total > 51      6grp_highest_sub > De      6grp_high1_sub > 02      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 797
1 2 3 4 range
A 1092
(1.3)
P 		1114.5
(1.6)
P 		1234.5
(3)
P 		724.2
(-3)
P 		510
B 1083.6
(0.3)
P 		1399
(3)
P 		1133.1
(0.8)
P 		689.6
(-3)
P 		709
C 1118.8
(0.5)
P 		1449.3
(3)
P 		968
(-0.6)
P 		652
(-3)
P 		797
avr 1098.1
(0.9) 		1320.9
(3) 		1111.9
(1) 		688.6
(-3) 		632

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 726
1 2 3 4 5 6 range
A 1175.2
(2.9)
P 		1132.2
(2.4)
P 		1181.1
(3)
P 		1080.2
(1.8)
P 		666.8
(-3)
P 		936.6
(0.1)
P 		514
B 916.3
(-1.8)
P 		1392.6
(3)
P 		924.4
(-1.7)
P 		1077
(-0.2)
P 		971.4
(-1.2)
P 		795.9
(-3)
P 		597
C 1384.4
(3)
P 		1234.4
(1.7)
P 		970.7
(-0.7)
P 		1192.8
(1.3)
P 		706.6
(-3)
P 		733.5
(-2.8)
P 		678
avr 1158.6
(1.8) 		1253.1
(3) 		1025.4
(0.1) 		1116.7
(1.3) 		781.6
(-3) 		822
(-2.5) 		472
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 311
1 2 3 4 range
A 397.6
(3)
P 		241.9
(-0.2)
P 		322.7
(1.5)
P 		106
(-3)
P 		292
B 345.5
(3)
P 		345.5
(3)
P 		262.6
(0.5)
P 		147
(-3)
P 		199
C 370.4
(2.1)
P 		416.7
(3)
P 		337.4
(1.4)
P 		109.9
(-3)
P 		307
avr 371.2
(3) 		334.7
(2.1) 		307.6
(1.5) 		121
(-3) 		250

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 312
1 2 3 4 5 6 range
A 216.6
(-2.5)
P 		402.7
(3)
P 		257.2
(-1.3)
P 		274
(-0.8)
P 		201.2
(-3)
P 		228
(-2.2)
P 		202
B 318.8
(-0.6)
P 		463.5
(3)
P 		224.5
(-3)
P 		285.3
(-1.5)
P 		394.7
(1.3)
P 		296.8
(-1.2)
P 		239
C 513.1
(3)
P 		376.7
(0.1)
P 		247.1
(-2.6)
P 		239.9
(-2.8)
P 		228.7
(-3)
P 		245.5
(-2.6)
P 		284
avr 349.5
(0.7) 		414.3
(3) 		242.9
(-3) 		266.4
(-2.2) 		274.9
(-1.9) 		256.8
(-2.5) 		171
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187200_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11114
Sequence Type Exemplar sequence
Representative Public ID E04A4.5 NCBI
Target Description E04A4.5 /REP_DB=WormBase Gene ID /WP=CE16966 /TR=SW:O44477 /GB=AAB92039.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068226(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068226 NCBI Caenorhabditis elegans E04A4.5 (E04A4.5) mRNA, complete cds. 11/11 None
SNAP00000038151 ENSEMBL cdna:SNAP chromosome:CEL140:IV:4739959:4745464:-1 11/11 None
GENEFINDER00000038160 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:4744709:4745464:-1 11/11 None
E04A4.5 ENSEMBL cdna:known chromosome:CEL140:IV:4744379:4745485:-1 gene:E04A4.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:4744714-4745470(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E04A4.5
Entrez Gene 177242 Entrez gene
SwissProt O44477 EMBL-EBI
WormBase CE16966 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:255941_AT mitochondrial import inner membrane translocase subunit Tim17, putative at
RAE230A:1370304_AT similar to translocator of inner mitochondrial membrane 17a rn
RAT230_2:1370304_AT similar to translocator of inner mitochondrial membrane 17a rn
YEAST_2:1778107_AT Essential constituent of the mitochondrial inner membrane presequence translocase; interacts with Pam18p to recruit the presequence translocase-associated motor (PAM complex) and also required for protein sorting during import Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
15031 protein transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5744 mitochondrial inner membrane presequence translocase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
15450 protein translocase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB92042 Homolog of elac2 (cancer susceptibility locus) protein 1, isoform a [Caenorhabditis elegans] ref|NP_001023109.1| Homolog Of ELAC2 (cancer susceptibility locus) family member (hoe-1) [Caenorhabditis elegans] sp|O44476|RNZ_CAEEL Ribonuclease Z (RNase Z) (tRNase Z) (tRNA 3 endonuclease) (Homolog of ELAC2 protein 1) (CeELAC2) 0.0
blast AAT27250 Homolog of elac2 (cancer susceptibility locus) protein 1, isoform b [Caenorhabditis elegans] ref|NP_001023110.1| Homolog Of ELAC2 (cancer susceptibility locus) family member (hoe-1) [Caenorhabditis elegans] 0.0
blast CAE58575 Hypothetical protein CBG01741 [Caenorhabditis briggsae] 0.0
blast AAB92039 Hypothetical protein E04A4.5 [Caenorhabditis elegans] ref|NP_500627.1| E04A4.5 [Caenorhabditis elegans] sp|O44477|TIM17_CAEEL Probable mitochondrial import inner membrane translocase subunit Tim17 1.0E-100
blast CAE58576 Hypothetical protein CBG01742 [Caenorhabditis briggsae] 2.0E-99
blast EAA11986 ENSANGP00000016107 [Anopheles gambiae str. PEST] ref|XP_316737.2| ENSANGP00000016107 [Anopheles gambiae str. PEST] 2.0E-42
Protein Domains
Database ID Description E-Value
Pfam IPR003397 EMBL-EBI Mitochondrial import inner membrane translocase, subunit Tim17/22 1.2E-56
Pfam IPR001279 EMBL-EBI Beta-lactamase-like 2.2E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500627.1 4 17-36,59-81,88-105,109-131
E04A4.5 4 17-36,59-81,88-105,109-131
SNAP00000038151 3 17-36,59-81,109-131
GENEFINDER00000038160 4 17-36,59-81,88-105,109-131
Sequence
Target Sequence 
>C. ELEGANS:187200_S_AT
gaagagtacaccagagagccatgcccatatcgtatcggagatgatattggatcggcgttt
gcgatgggtctcgttggaggatccattttccaagcttttggaggatataaaaatgcggcg
aaaggaaagaaattggttgggatgatgcgagaagtacgaatgagaagcacattaacagga
gttcagtttgccgcttggggaggaatgttcagtacaatcgattgttgtcttgtcgccatc
cgcaagaaggaagaccccatcaactctattgtttctggaggattgactggagctcttttg
gcgatccgttcaggaccaaaggtgatggcaggaagtgcgattcttggctctgtaattctg
gctatgatcgaaggagtcggattggtgaccacccgctggatgggagcaatgatggatcca
acacagccgccgccagaagctctcgacgatccacgcagtctgggacaaaagagtcaagca
gaaccaggactcgaccaaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGAGTACACCAGAGAGCCATGCC 636 337 16 Antisense
GCCATGCCCATATCGTATCGGAGAT 469 281 33 Antisense
TGGATCGGCGTTTGCGATGGGTCTC 244 551 63 Antisense
GGAGGATCCATTTTCCAAGCTTTTG 4 517 91 Antisense
ATTGACTGGAGCTCTTTTGGCGATC 662 5 297 Antisense
GGCGATCCGTTCAGGACCAAAGGTG 352 513 315 Antisense
GGCAGGAAGTGCGATTCTTGGCTCT 351 535 342 Antisense
CTTGGCTCTGTAATTCTGGCTATGA 10 219 358 Antisense
GAAGGAGTCGGATTGGTGACCACCC 611 333 385 Antisense
TGACCACCCGCTGGATGGGAGCAAT 664 569 401 Antisense
AAGCAGAACCAGGACTCGACCAAAC 212 151 491 Antisense
Sequence Source Affymetrix Proprietary Database