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[187194_at]

Description F08B4.5 /REP_DB=WormBase Gene ID /WP=CE07046 /TR=SW:Q19196 /GB=AAB37729.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 47      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 83      6grp_total > 47      4grp_total > 46      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 251
1 2 3 4 range
A 575.6
(3)
P 		457.8
(0.2)
P 		324.6
(-3)
P 		365.4
(-2)
P 		251
B 506.5
(3)
P 		405.8
(-0.5)
P 		420.8
(0.1)
P 		332
(-3)
P 		175
C 571.1
(3)
P 		410.6
(-1.2)
P 		347.2
(-2.9)
P 		343.9
(-3)
P 		227
avr 551.1
(3) 		424.7
(-0.7) 		364.2
(-2.5) 		347.1
(-3) 		204

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 639.3
(3)
P 		390.4
(-3)
P 		395.3
(-2.9)
P 		409.6
(-2.5)
P 		422.1
(-2.2)
P 		588.2
(1.8)
P 		249
B 459.4
(0.4)
P 		442.8
(-0.2)
P 		382.1
(-2.5)
P 		406.2
(-1.6)
P 		369.9
(-3)
P 		526.4
(3)
P 		157
C 408.2
(-0.6)
P 		484.2
(2.1)
P 		497.3
(2.6)
P 		341
(-3)
P 		421.3
(-0.1)
P 		508.5
(3)
P 		168
avr 502.3
(1.5) 		439.1
(-0.9) 		424.9
(-1.5) 		385.6
(-3) 		404.4
(-2.3) 		541
(3) 		155
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 156
1 2 3 4 range
A 209.6
(3)
P 		99.4
(-1.2)
P 		84.9
(-1.8)
P 		53.5
(-3)
P 		156
B 161.5
(3)
P 		100.2
(-1.1)
P 		97.5
(-1.2)
P 		70.7
(-3)
P 		91
C 189
(3)
P 		118.1
(-0.2)
P 		121
(-0.1)
P 		58
(-3)
P 		131
avr 186.7
(3) 		105.9
(-0.8) 		101.1
(-1.1) 		60.7
(-3) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 117.8
(0.3)
P 		138.9
(2.5)
P 		86.1
(-3)
P 		103.9
(-1.1)
P 		127.4
(1.3)
P 		143.2
(3)
P 		57
B 159.9
(0.9)
P 		147.4
(0.2)
P 		92.8
(-3)
P 		107.6
(-2.1)
P 		150.3
(0.3)
P 		196.3
(3)
P 		104
C 151.3
(1.9)
P 		147.7
(1.7)
P 		126.6
(0.4)
P 		68.6
(-3)
P 		136.3
(1)
P 		170.2
(3)
P 		102
avr 143
(0.9) 		144.7
(1) 		101.8
(-2.3) 		93.4
(-3) 		138
(0.5) 		169.9
(3) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187194_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9611
Sequence Type Exemplar sequence
Representative Public ID F08B4.5 NCBI
Target Description F08B4.5 /REP_DB=WormBase Gene ID /WP=CE07046 /TR=SW:Q19196 /GB=AAB37729.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069088(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069088 NCBI Caenorhabditis elegans F08B4.5 (F08B4.5) mRNA, complete cds. 11/11 None
SNAP00000041286 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8682554:8687791:-1 11/11 None
GENEFINDER00000041295 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8682554:8686592:-1 11/11 None
F08B4.5.2 ENSEMBL cdna:known chromosome:CEL140:IV:8682471:8686593:-1 gene:F08B4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8682476-8686576(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F08B4.5
Entrez Gene 177673 Entrez gene
SwissProt Q19196 EMBL-EBI
WormBase CE29749 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.21914.1.S1_S_AT similar to DNA polymerase epsilon subunit 2 cfa
DROSOPHILA_2:1630620_AT dm
DROSGENOME1:154339_AT dm
CHICKEN:GGAAFFX.12131.1.S1_S_AT polymerase (DNA directed), epsilon 2 (p59 subunit) gga
HG-U133_PLUS_2:205909_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HG-U133A:205909_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HG-FOCUS:205909_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HG-U133A_2:205909_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HU35KSUBD:RC_AA448664_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
U133_X3P:G4505934_3P_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HG-U95AV2:41085_AT polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HG-U95D:84336_AT Polymerase (DNA directed), epsilon 2 (p59 subunit) hs
HU35KSUBD:RC_AA351146_AT Polymerase (DNA directed), epsilon 2 (p59 subunit) hs
MOUSE430_2:1431440_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MOE430B:1431440_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MG-U74AV2:101920_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MOE430A:1427094_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MOUSE430A_2:1427094_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MOUSE430_2:1427094_AT polymerase (DNA directed), epsilon 2 (p59 subunit) mm
MU19KSUBA:TC19142_AT Polymerase (DNA directed), epsilon 2 (p59 subunit), mRNA (cDNA clone MGC:70176 IMAGE:30147626) mm
RG-U34C:RC_AI070935_AT polymerase (DNA directed), epsilon 2 (p59 subunit) (predicted) rn
RAE230B:1393982_AT polymerase (DNA directed), epsilon 2 (p59 subunit) (predicted) rn
RAT230_2:1393982_AT polymerase (DNA directed), epsilon 2 (p59 subunit) (predicted) rn
RAE230B:1396484_AT Polymerase (DNA directed), epsilon 2 (p59 subunit) (predicted) rn
RAT230_2:1396484_AT Polymerase (DNA directed), epsilon 2 (p59 subunit) (predicted) rn
YEAST_2:1770645_AT Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for normal yeast chromosomal replication; expression peaks at the G1/S phase boundary; potential Cdc28p substrate Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6260 DNA replication inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3887 DNA-directed DNA polymerase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37729 Hypothetical protein F08B4.5 [Caenorhabditis elegans] ref|NP_501489.2| F08B4.5 [Caenorhabditis elegans] sp|Q19196|DPOE2_CAEEL Putative DNA polymerase epsilon subunit B (DNA polymerase II subunit B) 0.0
blast T29484 hypothetical protein F08B4.5 - Caenorhabditis elegans 0.0
blast CAE72481 Hypothetical protein CBG19657 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007185 EMBL-EBI DNA polymerase epsilon subunit B 1.0E-86
Pfam IPR003604 EMBL-EBI Zn-finger, U1-like 2.0E-28
Pfam IPR007185 EMBL-EBI DNA polymerase epsilon subunit B 1.3E-84
Pfam IPR007185 EMBL-EBI DNA polymerase epsilon subunit B 2.4E-81
Sequence
Target Sequence 
>C. ELEGANS:187194_AT
agtttatctttgtaccaggacctgatgatccatttgttgacacggtacttcctcgtccac
atcttccatctcttctcttcaagcatatttccccaattatctcgtgtacatttgcttcga
atccgtgtcgaattcaattcgcaagccaggagattgttgtcttccgaagtgatttgatca
aaaaaacgtgcagacattcaatcaatagtatcactgtcgaatccatcccatcgagatatg
caagaagtgtgttgagccaggcgcatttgtgtccacttcctcaacatattacaccagttt
taccggatttctctcattccttgtcacttcatccacttcccgatcttctgatcactgctg
atcgtttcgagactttcaccgaaaaagtcgttggagctgacacaattgtctcaaatcctg
gttcattctctcgaagcaactacactttccacgtctattat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTTATCTTTGTACCAGGACCTGA 659 61 1034 Antisense
TTTGTTGACACGGTACTTCCTCGTC 465 665 1066 Antisense
TTTCCCCAATTATCTCGTGTACATT 650 669 1121 Antisense
TGTACATTTGCTTCGAATCCGTGTC 113 559 1138 Antisense
TAGTATCACTGTCGAATCCATCCCA 65 655 1239 Antisense
TGTCCACTTCCTCAACATATTACAC 107 557 1303 Antisense
TACACCAGTTTTACCGGATTTCTCT 612 643 1323 Antisense
CCCGATCTTCTGATCACTGCTGATC 212 261 1372 Antisense
GCTGATCGTTTCGAGACTTTCACCG 357 295 1390 Antisense
TCAAATCCTGGTTCATTCTCTCGAA 371 629 1444 Antisense
CAACTACACTTTCCACGTCTATTAT 195 189 1470 Antisense
Sequence Source Affymetrix Proprietary Database