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[187182_at]

Description EEED8.6 /REP_DB=WormBase Gene ID /WP=CE01890 /TR=SW:Q09296 /GB=AAC46766.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 34      4grp_total > 31      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 155
1 2 3 4 range
A 144.4
(3)
P 		10
(-3)
A 		12
(-2.9)
A 		110.3
(1.5)
A 		134
B 111.1
(3)
P 		73.5
(-1)
A 		54.7
(-3)
A 		56.8
(-2.8)
A 		56
C 164.5
(3)
P 		22.4
(-2.5)
A 		9.5
(-3)
A 		79.9
(-0.3)
A 		155
avr 140
(3) 		35.3
(-2.5) 		25.4
(-3) 		82.3
(-0) 		115

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 74
1 2 3 4 5 6 range
A 12.3
(-2.3)
A 		60.5
(3)
A 		26.2
(-0.8)
A 		10.5
(-2.5)
A 		45
(1.3)
A 		6.3
(-3)
A 		54
B 70.8
(3)
A 		17.7
(-2.2)
A 		66.8
(2.6)
A 		9.4
(-3)
A 		10.8
(-2.9)
A 		31.9
(-0.8)
A 		61
C 80.7
(3)
A 		16.5
(-2.3)
A 		51.1
(0.5)
A 		8.4
(-3)
A 		9.9
(-2.9)
A 		19
(-2.1)
A 		72
avr 54.6
(3) 		31.6
(-0.1) 		48
(2.1) 		9.4
(-3) 		21.9
(-1.3) 		19.1
(-1.7) 		45
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 52.6
(3)
P 		2.2
(-3)
A 		3.1
(-2.9)
A 		16.1
(-1.3)
A 		50
B 35.4
(3)
P 		18.2
(-1.4)
A 		12.7
(-2.8)
A 		12.1
(-3)
A 		23
C 54.4
(3)
P 		6.4
(-2.6)
A 		3.3
(-3)
A 		13.5
(-1.8)
A 		51
avr 47.5
(3) 		8.9
(-2.6) 		6.4
(-3) 		13.9
(-1.9) 		41

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 2.3
(-2.8)
A 		21.5
(3)
A 		5.7
(-1.7)
A 		2.7
(-2.6)
A 		13.6
(0.6)
A 		1.5
(-3)
A 		20
B 24.6
(3)
A 		5.9
(-2.1)
A 		16.2
(0.7)
A 		2.5
(-3)
A 		4.4
(-2.5)
A 		11.9
(-0.4)
A 		22
C 29.9
(3)
A 		5
(-2.3)
A 		13
(-0.6)
A 		1.7
(-3)
A 		3.2
(-2.7)
A 		6.4
(-2)
A 		28
avr 18.9
(3) 		10.8
(0.1) 		11.6
(0.4) 		2.3
(-3) 		7.1
(-1.3) 		6.6
(-1.4) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187182_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4164
Sequence Type Exemplar sequence
Representative Public ID EEED8.6 NCBI
Target Description EEED8.6 /REP_DB=WormBase Gene ID /WP=CE01890 /TR=SW:Q09296 /GB=AAC46766.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062611(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062611 NCBI Caenorhabditis elegans EEED8.6 (EEED8.6) mRNA, complete cds. 11/11 None
SNAP00000023164 ENSEMBL cdna:SNAP chromosome:CEL140:II:5384174:5387287:-1 9/11 None
GENEFINDER00000023185 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5384174:5387287:-1 10/11 None
EEED8.6 ENSEMBL cdna:known chromosome:CEL140:II:5384218:5387287:-1 gene:EEED8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5384215-5387285(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl EEED8.6
Entrez Gene 184043 Entrez gene
SwissProt Q09296 EMBL-EBI
WormBase CE01890 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1629729_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4182 carboxypeptidase A activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46766 Hypothetical protein EEED8.6 [Caenorhabditis elegans] ref|NP_495012.1| EEED8.6 [Caenorhabditis elegans] sp|Q09296|YQO6_CAEEL Hypothetical protein EEED8.6 in chromosome II 0.0
blast CAE59090 Hypothetical protein CBG02382 [Caenorhabditis briggsae] 1.0E-180
blast CAE59090 Hypothetical protein CBG02382 [Caenorhabditis briggsae] 1.0E-174
blast AAC46766 Hypothetical protein EEED8.6 [Caenorhabditis elegans] ref|NP_495012.1| EEED8.6 [Caenorhabditis elegans] sp|Q09296|YQO6_CAEEL Hypothetical protein EEED8.6 in chromosome II 1.0E-153
blast CAI17069 novel protein [Homo sapiens] emb|CAI14372.1| novel protein [Homo sapiens] emb|CAI19137.1| novel protein [Homo sapiens] emb|CAH70510.1| novel protein [Homo sapiens] emb|CAI13521.1| novel protein [Homo sapiens] emb|CAI23365.1| novel protein [Homo sapiens] emb|CAI22167.1| novel protein [Homo sapiens] 1.0E-108
blast CAE59090 Hypothetical protein CBG02382 [Caenorhabditis briggsae] 1.0E-103
blast CAI17069 novel protein [Homo sapiens] emb|CAI14372.1| novel protein [Homo sapiens] emb|CAI19137.1| novel protein [Homo sapiens] emb|CAH70510.1| novel protein [Homo sapiens] emb|CAI13521.1| novel protein [Homo sapiens] emb|CAI23365.1| novel protein [Homo sapiens] emb|CAI22167.1| novel protein [Homo sapiens] 3.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR000834 EMBL-EBI Peptidase M14, carboxypeptidase A 2.5E-82
Pfam IPR000834 EMBL-EBI Peptidase M14, carboxypeptidase A 2.5E-82
Pfam IPR000834 EMBL-EBI Peptidase M14, carboxypeptidase A 2.5E-82
Sequence
Target Sequence 
>C. ELEGANS:187182_AT
gggctcatccttcaatttatgctgtgaagaatttactgacgcaatatgataataatccgc
aagcccaaactgtaatctacgttgatctacatgcccattctcagaagccaaattgttttt
tgtatggaaatgtgaatatgtcagctcatttcacaattttaaaccctaatcttttcatag
tttcgctcaactttgcaatgaccacaaaatttaaattttctgaaacatttttttctaatt
ttcggtcgaagaaaaatcgactttccggcagttatggcttccgcatttgctggcagatct
ttccgaagattattcgctcgagttcactcaattcaacacggatgtggagaaggctggtac
tggcagaagaacgatgggagatctactttcctgcctttgctatacacttgaagtatcatt
cttctcttatcgccatacagactcttctggaaatggaatacaacattgtacgccatatct
tcaatataaatatgaagctctcggcgaggcattttgcagagcacttctcaacttctatga
agccgactgtggactacgagaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGCTCATCCTTCAATTTATGCTGT 522 493 827 Antisense
CTACATGCCCATTCTCAGAAGCCAA 157 227 913 Antisense
GTTTCGCTCAACTTTGCAATGACCA 418 451 1006 Antisense
CTTTCCGGCAGTTATGGCTTCCGCA 551 223 1087 Antisense
ATTTGCTGGCAGATCTTTCCGAAGA 542 17 1111 Antisense
GATTATTCGCTCGAGTTCACTCAAT 596 429 1134 Antisense
CGATGGGAGATCTACTTTCCTGCCT 581 249 1198 Antisense
TTTCCTGCCTTTGCTATACACTTGA 304 669 1213 Antisense
TCTTCTCTTATCGCCATACAGACTC 330 617 1246 Antisense
AGCTCTCGGCGAGGCATTTTGCAGA 10 81 1322 Antisense
GAAGCCGACTGTGGACTACGAGAGA 679 343 1365 Antisense
Sequence Source Affymetrix Proprietary Database