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[187181_at]

Description Y38A8.1 /REP_DB=WormBase Gene ID /WP=CE07570 /TR=SW:Q23236 /GB=AAA98017.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      4grp_highest_8_8 > 01      4grp_high_8_8 > 53      6grp_highest_nonorm > 53      6grp_total > 63      4grp_total > 50      6grp_highest_sub > 03      6grp_cgc > 92      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 446
1 2 3 4 range
A 620.1
(-2.1)
P 		729.2
(1.2)
P 		786.6
(3)
P 		592.1
(-3)
P 		195
B 643.9
(-1.3)
P 		962.4
(3)
P 		792.8
(0.7)
P 		516.7
(-3)
P 		446
C 678.4
(-0.3)
P 		864.3
(3)
P 		789.6
(1.7)
P 		526.3
(-3)
P 		338
avr 647.5
(-1) 		852
(3) 		789.7
(1.8) 		545
(-3) 		307

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 609
1 2 3 4 5 6 range
A 650.4
(-2.8)
P 		711.1
(-2.1)
P 		1121.8
(3)
P 		862.7
(-0.2)
P 		710
(-2.1)
P 		635.2
(-3)
P 		487
B 608.4
(-3)
P 		626.8
(-2.8)
P 		1109.3
(3)
P 		839.9
(-0.2)
P 		622.5
(-2.8)
P 		617.6
(-2.9)
P 		501
C 512.8
(-3)
P 		725.9
(-0.1)
P 		954.3
(3)
P 		879.6
(2)
P 		560.8
(-2.3)
P 		721.8
(-0.2)
P 		442
avr 590.5
(-3) 		687.9
(-1.8) 		1061.8
(3) 		860.7
(0.4) 		631.1
(-2.5) 		658.2
(-2.1) 		471
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 189
1 2 3 4 range
A 225.8
(3)
P 		158.3
(0.1)
P 		205.6
(2.1)
P 		86.7
(-3)
P 		139
B 205.3
(1.5)
P 		237.7
(3)
P 		183.7
(0.5)
P 		110.1
(-3)
P 		128
C 224.6
(1.4)
P 		248.5
(2.1)
P 		275.2
(3)
P 		88.7
(-3)
P 		187
avr 218.6
(2.9) 		214.8
(2.7) 		221.5
(3) 		95.2
(-3) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 150
1 2 3 4 5 6 range
A 119.9
(-3)
P 		252.9
(3)
P 		244.3
(2.6)
P 		218.8
(1.5)
P 		214.3
(1.3)
P 		154.7
(-1.4)
P 		133
B 211.7
(-2.7)
P 		208.6
(-3)
P 		269.4
(3)
P 		222.5
(-1.6)
P 		252.9
(1.4)
P 		230.3
(-0.9)
P 		61
C 190.1
(-1.8)
P 		221.5
(1.1)
P 		242.9
(3)
P 		176.9
(-3)
P 		181.5
(-2.6)
P 		241.6
(2.9)
P 		66
avr 173.9
(-3) 		227.7
(1.1) 		252.2
(3) 		206.1
(-0.5) 		216.2
(0.2) 		208.9
(-0.3) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187181_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4386
Sequence Type Exemplar sequence
Representative Public ID Y38A8.1 NCBI
Target Description Y38A8.1 /REP_DB=WormBase Gene ID /WP=CE07570 /TR=SW:Q23236 /GB=AAA98017.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062511(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062511 NCBI Caenorhabditis elegans NuDiX family member (ndx-3) (ndx-3) mRNA, complete cds. 11/11 None
SNAP00000028504 ENSEMBL cdna:SNAP chromosome:CEL140:II:4953580:4954401:1 11/11 None
GENEFINDER00000028509 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4953580:4954401:1 11/11 None
Y38A8.1 ENSEMBL cdna:known chromosome:CEL140:II:4953791:4954426:1 gene:Y38A8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4953788-4954399(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ndx-3
Ensembl Y38A8.1
Entrez Gene 173857 Entrez gene
SwissProt Q23236 EMBL-EBI
WormBase CE07570 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584087_AT similar to Nucleoside diphosphate-linked moiety X motif 8, mitochondrial precursor (Nudix motif 8) cfa
CANINE_2:CFA.4699.1.A1_AT similar to Nucleoside diphosphate-linked moiety X motif 8, mitochondrial precursor (Nudix motif 8) cfa
CANINE_2:CFA.4699.1.A1_S_AT similar to Nucleoside diphosphate-linked moiety X motif 8, mitochondrial precursor (Nudix motif 8) cfa
CANINE_2:CFAAFFX.17561.1.S1_AT similar to Nucleoside diphosphate-linked moiety X motif 8, mitochondrial precursor (Nudix motif 8) cfa
DROSOPHILA_2:1637401_AT dm
DROSGENOME1:141421_AT dm
MOE430A:1450111_A_AT nudix (nucleoside diphosphate linked moiety X)-type motif 8 mm
MOUSE430_2:1450111_A_AT nudix (nucleoside diphosphate linked moiety X)-type motif 8 mm
MOUSE430A_2:1450111_A_AT nudix (nucleoside diphosphate linked moiety X)-type motif 8 mm
MG-U74BV2:113592_AT Nudix (nucleoside diphosphate linked moiety X)-type motif 8 (Nudt8), mRNA mm
MU19KSUBA:TC23318_AT Nudix (nucleoside diphosphate linked moiety X)-type motif 8 (Nudt8), mRNA mm
Protein Similarities
Method ID Description E-Value
blast AAA98017 Nudix family protein 3 [Caenorhabditis elegans] ref|NP_494912.1| NuDiX family member (ndx-3) [Caenorhabditis elegans] sp|Q23236|NDX3_CAEEL Nudix hydrolase 1.0E-104
blast CAE59012 Hypothetical protein CBG02288 [Caenorhabditis briggsae] 3.0E-95
blast CAE59012 Hypothetical protein CBG02288 [Caenorhabditis briggsae] 4.0E-86
blast EAA04941 ENSANGP00000020516 [Anopheles gambiae str. PEST] ref|XP_309129.2| ENSANGP00000020516 [Anopheles gambiae str. PEST] 8.0E-24
Protein Domains
Database ID Description E-Value
Pfam IPR000086 EMBL-EBI NUDIX hydrolase 1.6E-19
Pfam IPR000086 EMBL-EBI NUDIX hydrolase 3.1E-21
Sequence
Target Sequence 
>C. ELEGANS:187181_AT
ctgattccgcttgtgactgttgacggtcgtgattctgtgcttctaacgaaacgatcgatt
catctcagatcgcatcgtggtgaagtatgttttccaggtggtcgaatggatccaggagaa
actacaactgaaactgctctccgtgaaacgtttgaagaaatcggggttaacgctgaaagt
gtggaaatctggggtcatttgaaaagtgtgataagacggcaagcggatttcaatgttact
ccgattgttggatacatttccgatgagcgagttctcgagaatctggtggtcaactctgat
gaagtacaagctgttttcacgattccaattgatgaacttatcaaaaaagccggactaact
aaattccaaagcaagagaatgaagtacacactgccttcgttcgattccacggagttcaag
gtccatcacaatgctccgaacgagtatcttcattccacccagcgagtttgggggctttct
ggtgtaatgttgcatcaagcattgaccctgctgaatccagatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGATTCCGCTTGTGACTGTTGACG 548 239 22 Antisense
CGGTCGTGATTCTGTGCTTCTAACG 569 243 45 Antisense
GATCGATTCATCTCAGATCGCATCG 652 417 74 Antisense
CTACAACTGAAACTGCTCTCCGTGA 160 227 143 Antisense
GTACAAGCTGTTTTCACGATTCCAA 373 461 325 Antisense
TGAAGTACACACTGCCTTCGTTCGA 249 573 401 Antisense
GATTCCACGGAGTTCAAGGTCCATC 435 433 424 Antisense
GCTCCGAACGAGTATCTTCATTCCA 664 297 454 Antisense
ATTCCACCCAGCGAGTTTGGGGGCT 441 1 473 Antisense
GGGGGCTTTCTGGTGTAATGTTGCA 557 497 491 Antisense
GCATTGACCCTGCTGAATCCAGATG 626 307 520 Antisense
Sequence Source Affymetrix Proprietary Database