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[187174_at]

Description C33H5.2 /REP_DB=WormBase Gene ID /WP=CE04146 /TR=SW:Q18416 /GB=AAA82261.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 43      4grp_high_8_8 > 20      6grp_total > 10      4grp_total > 11      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 27.9
(-3)
A 		50.8
(-1.4)
A 		32.6
(-2.7)
A 		116.1
(3)
P 		88
B 49
(-0.6)
P 		64.8
(0.8)
P 		22.6
(-3)
A 		89.2
(3)
P 		67
C 37.6
(-2.4)
A 		30
(-3)
A 		31.6
(-2.9)
P 		105.7
(3)
P 		76
avr 38.2
(-2.3) 		48.5
(-1.4) 		28.9
(-3) 		103.7
(3) 		75

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 801
1 2 3 4 5 6 range
A 64.9
(-3)
P 		718.5
(3)
P 		484
(0.8)
P 		363.9
(-0.3)
P 		242.1
(-1.4)
P 		102
(-2.7)
P 		654
B 21.8
(-3)
A 		664.9
(3)
P 		515.2
(1.6)
P 		296.5
(-0.4)
P 		247.3
(-0.9)
P 		91.3
(-2.4)
P 		643
C 31.8
(-3)
A 		822.6
(3)
P 		358
(-0.5)
P 		304.4
(-0.9)
P 		258.3
(-1.3)
P 		102.1
(-2.5)
P 		791
avr 39.5
(-3) 		735.3
(3) 		452.4
(0.6) 		321.6
(-0.6) 		249.2
(-1.2) 		98.5
(-2.5) 		696
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 10.2
(-1.8)
A 		11
(-1.2)
A 		8.5
(-3)
A 		17
(3)
P 		9
B 15.6
(1.5)
P 		16
(1.7)
P 		5.2
(-3)
A 		19
(3)
P 		14
C 12.4
(-0.5)
A 		8.6
(-3)
A 		11
(-1.4)
P 		17.8
(3)
P 		9
avr 12.7
(-0.2) 		11.9
(-0.7) 		8.2
(-3) 		17.9
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 248
1 2 3 4 5 6 range
A 12
(-3)
P 		255.5
(3)
P 		105.4
(-0.7)
P 		92.3
(-1)
P 		73.1
(-1.5)
P 		24.8
(-2.7)
P 		244
B 7.6
(-3)
A 		221.3
(3)
P 		125.1
(0.3)
P 		78.5
(-1)
P 		100.5
(-0.4)
P 		34
(-2.3)
P 		214
C 11.8
(-3)
A 		251
(3)
P 		91.1
(-1)
P 		61.2
(-1.8)
P 		83.6
(-1.2)
P 		34.2
(-2.4)
P 		239
avr 10.5
(-3) 		242.6
(3) 		107.2
(-0.5) 		77.3
(-1.3) 		85.7
(-1.1) 		31
(-2.5) 		232
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187174_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8913
Sequence Type Exemplar sequence
Representative Public ID C33H5.2 NCBI
Target Description C33H5.2 /REP_DB=WormBase Gene ID /WP=CE04146 /TR=SW:Q18416 /GB=AAA82261.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068893(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068893 NCBI Caenorhabditis elegans C33H5.2 (C33H5.2) mRNA, complete cds. 11/11 None
SNAP00000004488 ENSEMBL cdna:SNAP chromosome:CEL140:IV:7805048:7807931:1 11/11 None
GENEFINDER00000004500 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:7805048:7807931:1 11/11 None
C33H5.2 ENSEMBL cdna:known chromosome:CEL140:IV:7805048:7807931:1 gene:C33H5.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7805053-7807937(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C33H5.2
Entrez Gene 183184 Entrez gene
SwissProt Q18416 EMBL-EBI
WormBase CE04146 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA82261 Hypothetical protein C33H5.2 [Caenorhabditis elegans] ref|NP_501294.1| C33H5.2 [Caenorhabditis elegans] sp|Q18416|YY42_CAEEL Hypothetical protein C33H5.2 in chromosome IV 0.0
blast CAE64028 Hypothetical protein CBG08623 [Caenorhabditis briggsae] 1.0E-175
blast CAA92519 Hypothetical protein F49C12.5a [Caenorhabditis elegans] ref|NP_501627.1| F49C12.5a [Caenorhabditis elegans] sp|Q20593|YE25_CAEEL Hypothetical protein F49C12.5 in chromosome IV 1.0E-173
Protein Domains
Database ID Description E-Value
Pfam IPR008166 EMBL-EBI Protein of unknown function DUF23 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501294.1 1 7-29
C33H5.2 1 7-29
SNAP00000004488 1 7-29
GENEFINDER00000004500 1 7-29
Sequence
Target Sequence 
>C. ELEGANS:187174_AT
gacaccaaatagctctcaattttcattgaaaaatatgttcggcagtttgaaatgcagaaa
ctttagagaaacaggaaaaagtgtaattgatccacaaaacgccaattacacgtggcttca
ttatccaccggttctagtgaatggattggaaaaatatgaggttgaggagaatgtgattac
ccatttgaagacaattaattgggttgaagacgaagtaaaaaccggaaatggcacaattat
tgagccaatgtactatgacaattcatctgccacaataatttctagcaaagatatcaaaga
tattgaagatgatctacaaagaatgagaaataaaccagaaataaagaaactattcgcaga
acttccaaaaattcgatactattcggatctcgttctcaaatgctacaacgaaaagttcta
tgactatttttattcaggaagatatgaaaaaatcacttgtcctggaccacagtattgcga
tttcaagcagcatccggatatcacgtgtatgcgtgtaaatgcaacacatattgaacgaga
aacgttgtctccagtcacttatta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACACCAAATAGCTCTCAATTTTCA 564 365 921 Antisense
TGATCCACAAAACGCCAATTACACG 607 563 1008 Antisense
ACACGTGGCTTCATTATCCACCGGT 113 111 1028 Antisense
ATTATCCACCGGTTCTAGTGAATGG 610 1 1040 Antisense
GTACTATGACAATTCATCTGCCACA 459 459 1170 Antisense
GAAACTATTCGCAGAACTTCCAAAA 635 355 1266 Antisense
AATTCGATACTATTCGGATCTCGTT 670 177 1290 Antisense
GAAAAAATCACTTGTCCTGGACCAC 451 353 1366 Antisense
CCTGGACCACAGTATTGCGATTTCA 598 265 1381 Antisense
GCAGCATCCGGATATCACGTGTATG 106 313 1407 Antisense
AAACGTTGTCTCCAGTCACTTATTA 298 125 1460 Antisense
Sequence Source Affymetrix Proprietary Database