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[187173_at]

Description Y17G7B.4 /REP_DB=WormBase Gene ID /WP=CE19037 /TR=SW:Q9XXJ0 /GB=CAA19451.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 28      4grp_high_8_8 > 23      6grp_highest_nonorm > 92      6grp_total > 77      4grp_total > 0      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 219
1 2 3 4 range
A 320.3
(-3)
P 		371.1
(3)
P 		341.6
(-0.5)
P 		335.6
(-1.2)
P 		51
B 341.4
(1.7)
P 		176.5
(-3)
P 		368.7
(2.5)
P 		386.4
(3)
P 		210
C 302.1
(-3)
P 		342.1
(-0.4)
P 		379.1
(2)
P 		395
(3)
P 		93
avr 321.3
(-1) 		296.6
(-3) 		363.1
(2.3) 		372.3
(3) 		76

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 423
1 2 3 4 5 6 range
A 207.3
(-2.1)
P 		160.7
(-3)
P 		168.8
(-2.8)
P 		210.1
(-2)
P 		263.3
(-1)
P 		463.3
(3)
P 		303
B 265.1
(-1)
P 		171.8
(-3)
P 		229.4
(-1.7)
P 		241.9
(-1.5)
P 		269.5
(-0.9)
P 		445.7
(3)
P 		274
C 167.7
(-3)
P 		249
(-1.8)
P 		209.2
(-2.4)
P 		204.7
(-2.5)
P 		272.5
(-1.5)
P 		583.5
(3)
P 		416
avr 213.4
(-2.6) 		193.8
(-3) 		202.5
(-2.8) 		218.9
(-2.5) 		268.4
(-1.5) 		497.5
(3) 		304
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 89
1 2 3 4 range
A 116.6
(3)
P 		80.6
(-0.2)
P 		89.3
(0.6)
P 		49.1
(-3)
P 		68
B 108.9
(3)
P 		43.6
(-3)
P 		85.4
(0.8)
P 		82.3
(0.6)
P 		65
C 100
(0.1)
P 		98.4
(-0.1)
P 		132.1
(3)
P 		66.6
(-3)
P 		66
avr 108.5
(3) 		74.2
(-1.8) 		102.3
(2.1) 		66
(-3) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 159
1 2 3 4 5 6 range
A 38.2
(-2.9)
P 		57.2
(-1.4)
P 		36.8
(-3)
P 		53.3
(-1.7)
P 		79.5
(0.4)
P 		112.8
(3)
P 		76
B 92.2
(-1)
P 		57.2
(-2.9)
P 		55.7
(-3)
P 		64.1
(-2.5)
P 		109.5
(-0.1)
P 		166.2
(3)
P 		111
C 62.2
(-2.2)
P 		76
(-1.6)
P 		53.3
(-2.5)
P 		41.2
(-3)
P 		88.2
(-1.2)
P 		195.3
(3)
P 		154
avr 64.2
(-2.1) 		63.5
(-2.2) 		48.6
(-3) 		52.9
(-2.8) 		92.4
(-0.6) 		158.1
(3) 		110
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187173_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3614
Sequence Type Exemplar sequence
Representative Public ID Y17G7B.4 NCBI
Target Description Y17G7B.4 /REP_DB=WormBase Gene ID /WP=CE19037 /TR=SW:Q9XXJ0 /GB=CAA19451.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064156(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064156 NCBI Caenorhabditis elegans Y17G7B.4 (Y17G7B.4) mRNA, complete cds. 11/11 None
SNAP00000030317 ENSEMBL cdna:SNAP chromosome:CEL140:II:11980378:12001314:1 10/11 None
GENEFINDER00000030409 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:11993720:11997805:1 11/11 None
Y17G7B.4 ENSEMBL cdna:known chromosome:CEL140:II:11992911:11997904:1 gene:Y17G7B.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:11992914-11997802(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y17G7B.4
Entrez Gene 174840 Entrez gene
SwissProt Q9XXJ0 EMBL-EBI
WormBase CE19037 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250706_AT deoxyhypusine synthase at
CANINE:1604007_AT similar to deoxyhypusine synthase cfa
CANINE_2:CFA.1439.2.S1_AT similar to deoxyhypusine synthase cfa
CANINE_2:CFA.1439.2.S1_S_AT similar to deoxyhypusine synthase cfa
DROSOPHILA_2:1631965_AT dm
DROSGENOME1:148379_AT dm
HG-U133_PLUS_2:211558_S_AT deoxyhypusine synthase hs
HG-FOCUS:211558_S_AT deoxyhypusine synthase hs
HG-U133A:211558_S_AT deoxyhypusine synthase hs
HG-U133A_2:211558_S_AT deoxyhypusine synthase hs
HG-U95AV2:37722_S_AT deoxyhypusine synthase hs
HG-U133_PLUS_2:207831_X_AT deoxyhypusine synthase hs
HG-U133A:207831_X_AT deoxyhypusine synthase hs
HG-U133A_2:207831_X_AT deoxyhypusine synthase hs
HG-U133_PLUS_2:202802_AT deoxyhypusine synthase hs
HG-U133A:202802_AT deoxyhypusine synthase hs
HG-U133A_2:202802_AT deoxyhypusine synthase hs
HUGENEFL:U79262_AT deoxyhypusine synthase hs
HUGENEFL:U26266_S_AT deoxyhypusine synthase hs
U133_X3P:G7108339_3P_S_AT deoxyhypusine synthase hs
U133_X3P:G1373016_3P_S_AT deoxyhypusine synthase hs
HG-U95AV2:37721_AT deoxyhypusine synthase hs
MOE430A:1434004_AT deoxyhypusine synthase mm
MOUSE430A_2:1434004_AT deoxyhypusine synthase mm
MOUSE430_2:1434004_AT deoxyhypusine synthase mm
MU11KSUBA:AA270493_AT deoxyhypusine synthase mm
MG-U74BV2:111978_AT deoxyhypusine synthase mm
MG-U74BV2:111977_S_AT deoxyhypusine synthase mm
MG-U74BV2:104788_AT deoxyhypusine synthase mm
MG-U74AV2:160405_AT deoxyhypusine synthase mm
MOE430A:1434003_A_AT deoxyhypusine synthase mm
MOUSE430A_2:1434003_A_AT deoxyhypusine synthase mm
MOUSE430_2:1434003_A_AT deoxyhypusine synthase mm
MU19KSUBA:TC14359_AT Deoxyhypusine synthase (Dhps), mRNA mm
MU19KSUBC:TC37476_AT Deoxyhypusine synthase (Dhps), mRNA mm
MU19KSUBC:TC37477_AT Deoxyhypusine synthase (Dhps), mRNA mm
RG-U34B:RC_AI013060_AT deoxyhypusine synthase rn
RG-U34C:RC_AI137356_AT deoxyhypusine synthase rn
RAE230A:1388803_AT deoxyhypusine synthase rn
RAT230_2:1388803_AT deoxyhypusine synthase rn
YEAST_2:1775237_AT Deoxyhypusine synthase, catalyzes formation of deoxyhypusine, the first step in hypusine biosynthesis; triggers posttranslational hypusination of translation elongation factor eIF-5A and regulates its intracellular levels; tetrameric Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8612 hypusine biosynthesis from peptidyl-lysine inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA19451 Hypothetical protein Y17G7B.4 [Caenorhabditis elegans] ref|NP_496557.1| Y17G7B.4 [Caenorhabditis elegans] sp|Q9XXJ0|DHYS_CAEEL Probable deoxyhypusine synthase (DHS) 0.0
blast CAE73555 Hypothetical protein CBG21024 [Caenorhabditis briggsae] 0.0
blast CAA19449 Hypothetical protein Y17G7B.2a [Caenorhabditis elegans] ref|NP_496555.1| Y17G7B.2a [Caenorhabditis elegans] 0.0
blast CAA19468 Hypothetical protein Y17G7B.2b [Caenorhabditis elegans] ref|NP_496553.1| Y17G7B.2b [Caenorhabditis elegans] 0.0
blast CAE73557 Hypothetical protein CBG21026 [Caenorhabditis briggsae] 0.0
blast CAA19451 Hypothetical protein Y17G7B.4 [Caenorhabditis elegans] ref|NP_496557.1| Y17G7B.4 [Caenorhabditis elegans] sp|Q9XXJ0|DHYS_CAEEL Probable deoxyhypusine synthase (DHS) 1.0E-173
blast CAE73555 Hypothetical protein CBG21024 [Caenorhabditis briggsae] 1.0E-153
blast AAF50467 CG8005-PA [Drosophila melanogaster] gb|AAQ23547.1| RE64695p [Drosophila melanogaster] ref|NP_648188.2| CG8005-PA [Drosophila melanogaster] sp|Q9VSF4|DHYS_DROME Probable deoxyhypusine synthase (DHS) 1.0E-119
Protein Domains
Database ID Description E-Value
Pfam IPR002773 EMBL-EBI Deoxyhypusine synthase 1.0E-126
Pfam IPR003877 EMBL-EBI SPla/RYanodine receptor SPRY 2.8E-15
Pfam IPR003877 EMBL-EBI SPla/RYanodine receptor SPRY 7.6E-11
Pfam IPR001279 EMBL-EBI Beta-lactamase-like 4.6E-28
Pfam IPR002773 EMBL-EBI Deoxyhypusine synthase 1.0E-126
Pfam IPR002773 EMBL-EBI Deoxyhypusine synthase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:187173_AT
atcgagccggcaatgtgctcattccgaatgataattattgtgcatttgaggattggctta
tgccaattcttgatgaatgtcttgttgaacaagaggaaaagcatctcaattggacaccgt
caaagcttattcaacggcttggtgaacggattggtgatgaatcgtcgatactgtattggg
cggccaagcatcggattcccgtattttgtccggcgcttactgatgggtcattgggtgaca
tgttgtacttccattcggtgaaaagctcacctgggcttcgtgtggatattgtagaggacg
ttcgacatattaatacaattgctgtcaaaagtttcaagactggatcaattattttaggag
gaggagtcgtaaaacaccacatcaacaacgcgaatctcatgagaaacggcgcagatcata
ccgtctacatcaacaccggccaagaattcgatggctccgattctggagctcaacccgacg
aggcagtgagctgggggaaggttaagccgtcggctggagctgtgaaggttcatgctgagg
cgacacttgtattcccacttttggtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCGAGCCGGCAATGTGCTCATTCC 323 37 524 Antisense
AAAGCATCTCAATTGGACACCGTCA 524 123 621 Antisense
AGCATCGGATTCCCGTATTTTGTCC 569 77 710 Antisense
GACATGTTGTACTTCCATTCGGTGA 376 369 760 Antisense
CTCACCTGGGCTTCGTGTGGATATT 356 229 789 Antisense
ACCACATCAACAACGCGAATCTCAT 550 85 899 Antisense
CGCAGATCATACCGTCTACATCAAC 46 255 933 Antisense
TGGCTCCGATTCTGGAGCTCAACCC 449 545 975 Antisense
TAAGCCGTCGGCTGGAGCTGTGAAG 411 637 1026 Antisense
GGTTCATGCTGAGGCGACACTTGTA 309 507 1050 Antisense
GACACTTGTATTCCCACTTTTGGTT 276 365 1065 Antisense
Sequence Source Affymetrix Proprietary Database