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[187171_s_at]

Description Y110A7A.10 /REP_DB=WormBase Gene ID /WP=CE23248 /TR=Q9N597 /GB=AAF60427.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 78      4grp_highest_8_8 > 45      4grp_high_8_8 > 08      6grp_highest_nonorm > 74      6grp_total > 59      4grp_total > 23      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 290
1 2 3 4 range
A 779.4
(2.2)
P 		529.4
(-3)
P 		614.7
(-1.2)
P 		815.3
(3)
P 		286
B 819.8
(3)
P 		596.4
(-2.8)
P 		589.6
(-3)
P 		704.3
(-0)
P 		230
C 743.7
(3)
P 		558.1
(-3)
P 		613.5
(-1.2)
P 		629.1
(-0.7)
P 		186
avr 781
(3) 		561.3
(-3) 		605.9
(-1.8) 		716.2
(1.2) 		220

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 352
1 2 3 4 5 6 range
A 820.8
(2.4)
P 		553.1
(-2.5)
P 		523.9
(-3)
P 		639.8
(-0.9)
P 		668.9
(-0.4)
P 		852.3
(3)
P 		328
B 723.3
(1.3)
P 		539.1
(-2.6)
P 		521.4
(-3)
P 		676.4
(0.3)
P 		637.1
(-0.5)
P 		804.3
(3)
P 		283
C 647.3
(-1.3)
P 		558.9
(-3)
P 		659.2
(-1.1)
P 		692.2
(-0.5)
P 		698
(-0.3)
P 		873.4
(3)
P 		315
avr 730.5
(0.7) 		550.4
(-3) 		568.2
(-2.6) 		669.5
(-0.6) 		668
(-0.6) 		843.3
(3) 		293
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 178
1 2 3 4 range
A 283.8
(3)
P 		114.9
(-3)
P 		160.7
(-1.4)
P 		119.3
(-2.8)
P 		169
B 261.4
(3)
P 		147.3
(-2.5)
P 		136.6
(-3)
P 		150.1
(-2.4)
P 		125
C 246.2
(3)
P 		160.4
(-0.7)
P 		213.8
(1.6)
P 		106.1
(-3)
P 		140
avr 263.8
(3) 		140.9
(-2.3) 		170.4
(-1) 		125.2
(-3) 		139

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 186
1 2 3 4 5 6 range
A 151.3
(-0.6)
P 		196.7
(2.3)
P 		114.1
(-3)
P 		162.3
(0.1)
P 		201.8
(2.6)
P 		207.5
(3)
P 		93
B 251.7
(1.3)
P 		179.4
(-1.2)
P 		126.6
(-3)
P 		179.2
(-1.2)
P 		258.9
(1.6)
P 		299.9
(3)
P 		173
C 239.9
(0.9)
P 		170.5
(-1.8)
P 		167.8
(-1.9)
P 		139.2
(-3)
P 		225.9
(0.4)
P 		292.4
(3)
P 		153
avr 214.3
(0.6) 		182.2
(-0.9) 		136.2
(-3) 		160.2
(-1.9) 		228.9
(1.3) 		266.6
(3) 		130
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187171_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1439
Sequence Type Exemplar sequence
Representative Public ID Y110A7A.10 NCBI
Target Description Y110A7A.10 /REP_DB=WormBase Gene ID /WP=CE23248 /TR=Q9N597 /GB=AAF60427.2 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059121(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059121 NCBI Caenorhabditis elegans phosphoinositide kinase AdAPter subunit family member (aap-1) (aap-1) mRNA, complete cds. 11/11 None
GENEFINDER00000029232 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:5106185:5109950:1 11/11 None
SNAP00000029205 ENSEMBL cdna:SNAP chromosome:CEL140:I:5107847:5109950:1 11/11 None
Y110A7A.10 ENSEMBL cdna:known chromosome:CEL140:I:5107846:5110167:1 gene:Y110A7A.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5107846-5109950(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol aap-1
Ensembl Y110A7A.10
Entrez Gene 172141 Entrez gene
SwissProt Q9N597 EMBL-EBI
WormBase CE23248 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1589157_AT similar to Phosphatidylinositol 3-kinase regulatory gamma subunit (PI3-kinase p85-gamma subunit) (PtdIns-3-kinase p85-gamma) (p55PIK) cfa
CANINE_2:CFA.11616.1.A1_AT similar to Phosphatidylinositol 3-kinase regulatory gamma subunit (PI3-kinase p85-gamma subunit) (PtdIns-3-kinase p85-gamma) (p55PIK) cfa
CANINE_2:CFAAFFX.7417.1.S1_S_AT similar to Phosphatidylinositol 3-kinase regulatory gamma subunit (PI3-kinase p85-gamma subunit) (PtdIns-3-kinase p85-gamma) (p55PIK) cfa
CANINE_2:CFAAFFX.7426.1.S1_S_AT similar to Phosphatidylinositol 3-kinase regulatory gamma subunit (PI3-kinase p85-gamma subunit) (PtdIns-3-kinase p85-gamma) (p55PIK) cfa
DROSOPHILA_2:1622909_AT dm
DROSGENOME1:154995_AT dm
CHICKEN:GGAAFFX.6526.1.S1_S_AT similar to phosphatidylinositol 3-kinase p55 gamma regulatory subunit gga
CHICKEN:GGAAFFX.6526.7.S1_AT similar to phosphatidylinositol 3-kinase p55 gamma regulatory subunit gga
CHICKEN:GGAAFFX.6526.7.S1_S_AT similar to phosphatidylinositol 3-kinase p55 gamma regulatory subunit gga
HG-U95AV2:322_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HC-G110:322_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HG-U133_PLUS_2:211580_S_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HG-U133A:211580_S_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HG-U133A_2:211580_S_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HUGENEFL:U90907_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HUGENEFL:D88532_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
U133_X3P:G3046405_3P_A_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
U133_X3P:HS.88051.0.S2_3P_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HG-U133A_2:202743_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HG-FOCUS:202743_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HG-U133_PLUS_2:202743_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HG-U133A:202743_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HG-U95AV2:34479_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
HG-U95AV2:37961_AT phosphoinositide-3-kinase, regulatory subunit 3 (p55, gamma) hs
MOE430B:1456482_AT phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55) mm
MOUSE430_2:1456482_AT phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55) mm
MU19KSUBC:TC35339_AT Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 (p55) (Pik3r3), mRNA mm
RG-U34A:D64047_AT phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 rn
RAE230A:1369518_AT phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 rn
RAT230_2:1369518_AT phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 3 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7242 intracellular signaling cascade inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5623 cell inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
19901 protein kinase binding inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAF60427 Phosphoinositide kinase adapter subunit protein 1 [Caenorhabditis elegans] ref|NP_491522.1| phosphoinositide kinase AdAPter subunit family member (aap-1) [Caenorhabditis elegans] gb|AAF28335.1| phosphoinositide 3-kinase adapter subunit [Caenorhabditis elegans] 0.0
blast CAE65732 Hypothetical protein CBG10815 [Caenorhabditis briggsae] 0.0
blast AAF60428 Hypothetical protein Y110A7A.11 [Caenorhabditis elegans] ref|NP_491521.2| Y110A7A.11 [Caenorhabditis elegans] sp|Q9N598|USE1_CAEEL USE1-like protein 1.0E-100
blast AAH26146 Pik3r1 protein [Mus musculus] 3.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR000980 EMBL-EBI SH2 motif 1.8E-17
Pfam IPR000980 EMBL-EBI SH2 motif 1.3E-10
Pfam IPR000980 EMBL-EBI SH2 motif 1.8E-17
Pfam IPR000980 EMBL-EBI SH2 motif 1.3E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000029232 2 4-26,217-234
Sequence
Target Sequence 
>C. ELEGANS:187171_S_AT
tctgattcatgctcgctatcggatcgctcaacttgacaaggctgttggattgaaaagaga
gccacaagatggtattttcttgattcgaggatcctcttctcatggtgataagctcgtatt
gagtgtacttcacggagaacgtgtctcacattgtctaatcgaacaaaacgaagaaggatg
gggattcgagcacagcaatgtctacctgacaactataagtgacttcgttcgctattactc
tcacttcagtcttgaaacccacgcggatgctatcaaaactccacttcgtacgcccgcatt
tgacgctgtcacaagtgatacttcaaaacctcttcgtaacggacccggacaagtttttac
ggctttgccaatatccaggaaatatatggagaaggctcttctcactgatgatcccactaa
gccacctgaatcaccggaaccaagaccggattcatgggattctccatctccggatactcc
ttcatcatctgattcccgattggttcatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTGATTCATGCTCGCTATCGGATC 217 607 1176 Antisense
CTATCGGATCGCTCAACTTGACAAG 151 225 1191 Antisense
GATTCGAGGATCCTCTTCTCATGGT 271 433 1257 Antisense
GATTCGAGCACAGCAATGTCTACCT 256 433 1358 Antisense
AAGTGACTTCGTTCGCTATTACTCT 188 159 1392 Antisense
CTATTACTCTCACTTCAGTCTTGAA 366 223 1407 Antisense
TCTTGAAACCCACGCGGATGCTATC 511 609 1425 Antisense
GCTATCAAAACTCCACTTCGTACGC 245 303 1444 Antisense
GATACTTCAAAACCTCTTCGTAACG 246 423 1492 Antisense
TCTTCTCACTGATGATCCCACTAAG 266 617 1572 Antisense
CATCTGATTCCCGATTGGTTCATCC 605 211 1661 Antisense
Sequence Source Affymetrix Proprietary Database