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[187163_at]

Description Y65B4BR.4 /REP_DB=WormBase Gene ID /WP=CE25540 /TR=Q9N2Z7 /GB=AAF60858.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 18      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 10      6grp_total > 16      4grp_total > 38      6grp_highest_sub > De      6grp_cgc > 03      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 491
1 2 3 4 range
A 1244.2
(3)
P 		908.7
(-2.8)
P 		895.4
(-3)
P 		996.9
(-1.3)
P 		349
B 1127.2
(2.6)
P 		928.3
(-2.3)
P 		901.2
(-3)
P 		1142.1
(3)
P 		241
C 1195.2
(3)
P 		1076.3
(1.4)
P 		910.9
(-0.9)
P 		753.5
(-3)
P 		442
avr 1188.9
(3) 		971.1
(-1.6) 		902.5
(-3) 		964.2
(-1.7) 		286

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1315
1 2 3 4 5 6 range
A 1615.9
(3)
P 		1376.6
(1.3)
P 		796
(-2.9)
P 		788.2
(-3)
P 		1180.4
(-0.2)
P 		1154.7
(-0.3)
P 		828
B 1737.1
(3)
P 		1299.3
(-0)
P 		863.8
(-3)
P 		1103.3
(-1.4)
P 		1133.6
(-1.1)
P 		1008
(-2)
P 		873
C 2102.8
(3)
P 		1382.1
(-0.3)
P 		965.5
(-2.2)
P 		947.6
(-2.3)
P 		1090.7
(-1.7)
P 		802.1
(-3)
P 		1301
avr 1818.6
(3) 		1352.7
(0) 		875.1
(-3) 		946.4
(-2.5) 		1134.9
(-1.3) 		988.3
(-2.3) 		944
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 326
1 2 3 4 range
A 453
(3)
P 		197.3
(-2)
P 		234.1
(-1.3)
P 		145.9
(-3)
P 		307
B 359.5
(3)
P 		229.3
(-2.2)
P 		208.8
(-3)
P 		243.4
(-1.6)
P 		151
C 395.7
(3)
P 		309.5
(1.1)
P 		317.5
(1.3)
P 		127
(-3)
P 		269
avr 402.7
(3) 		245.4
(-1.1) 		253.5
(-0.9) 		172.1
(-3) 		231

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 606
1 2 3 4 5 6 range
A 297.9
(-0.6)
P 		489.6
(3)
P 		173.4
(-3)
P 		199.9
(-2.5)
P 		356.2
(0.5)
P 		281.1
(-1)
P 		316
B 604.4
(3)
P 		432.5
(0.4)
P 		209.8
(-3)
P 		292.2
(-1.7)
P 		460.6
(0.8)
P 		375.9
(-0.5)
P 		395
C 779.4
(3)
P 		421.7
(-0.6)
P 		245.7
(-2.4)
P 		190.6
(-3)
P 		352.9
(-1.3)
P 		268.5
(-2.2)
P 		589
avr 560.6
(3) 		447.9
(1.1) 		209.6
(-3) 		227.6
(-2.7) 		389.9
(0.1) 		308.5
(-1.3) 		351
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187163_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2555
Sequence Type Exemplar sequence
Representative Public ID Y65B4BR.4 NCBI
Target Description Y65B4BR.4 /REP_DB=WormBase Gene ID /WP=CE25540 /TR=Q9N2Z7 /GB=AAF60858.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170794(11), NM_171831(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170794 NCBI Caenorhabditis elegans WW domain Protein (E3 ubiquitin ligase) family member (wwp-1) (wwp-1) mRNA, complete cds. 11/11 None
NM_171831 NCBI Caenorhabditis elegans WW domain Protein (E3 ubiquitin ligase) family member (wwp-1) (wwp-1) mRNA, complete cds. 11/11 None
GENEFINDER00000036995 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:535811:541634:1 11/11 None
SNAP00000036980 ENSEMBL cdna:SNAP chromosome:CEL140:I:537141:541634:1 11/11 None
Y65B4BR.4a ENSEMBL cdna:known chromosome:CEL140:I:537125:542192:1 gene:Y65B4BR.4 11/11 A 
Y65B4BR.4b ENSEMBL cdna:known chromosome:CEL140:I:537141:541634:1 gene:Y65B4BR.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:537140-541634(+) 98.61 98.61
Public Domain and Genome References
Ensembl Y65B4BR.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN73849 Ww domain protein (e3 ubiquitin ligase) protein 1, isoform a [Caenorhabditis elegans] ref|NP_740775.1| WW domain Protein (E3 ubiquitin ligase) family member (wwp-1) [Caenorhabditis elegans] 0.0
blast AAN73850 Ww domain protein (e3 ubiquitin ligase) protein 1, isoform b [Caenorhabditis elegans] ref|NP_740776.1| WW domain Protein (E3 ubiquitin ligase) family member (wwp-1) [Caenorhabditis elegans] 0.0
blast CAE61291 Hypothetical protein CBG05115 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 3.5E-15
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 6.0E-16
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 8.9E-13
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 2.5E-12
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 1.0E-126
Pfam IPR000008 EMBL-EBI C2 domain 3.1E-7
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 2.5E-12
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 1.0E-126
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 1.6E-6
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 3.5E-15
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 6.0E-16
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 8.9E-13
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 2.5E-12
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 2.8E-16
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 1.1E-102
Pfam IPR000008 EMBL-EBI C2 domain 3.1E-7
Pfam IPR002715 EMBL-EBI Nascent polypeptide-associated complex NAC 2.4E-24
Sequence
Target Sequence 
>C. ELEGANS:187163_AT
ggagctctattttgttgccgattacgagctgctcggcgagctcaagacttatgagcttaa
ggagggcggtacagagattgctgttaccgaggagaataagcttgaatacatcgaactgct
cgttgagtggcgcttcaatcgcggtgtcgaacaacagacaaaagccttcttcaccggctt
caactcggtcttcccgttggaatggatgcagtatttcgatgaaagagagctcgagctgtt
gctctgcggaatgcaggacgttgatgtggacgattggcagagaaatactgtctacagaca
ttatgctccacagagcaagcaggtaacctggttctggcaatgggttcgaagtctggacca
agaaaaacgtgcccggctcctacaattcgtcacaggaacgtgccgtgtgccagtcggtgg
attttccgagctgatgggctcgacgggaccacaactattctgtatcgagcgtgtcggcaa
ggagaactggctcccacggtcgcatacgtgcttcaatcgactcgacttgccgccatacag
aagctacgatcag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGCTCTATTTTGTTGCCGATTAC 553 521 1857 Antisense
TCGAACTGCTCGTTGAGTGGCGCTT 572 599 1967 Antisense
ACTCGGTCTTCCCGTTGGAATGGAT 455 1 2039 Antisense
AGAGAGCTCGAGCTGTTGCTCTGCG 498 69 2080 Antisense
GTCTACAGACATTATGCTCCACAGA 79 469 2146 Antisense
TCGTCACAGGAACGTGCCGTGTGCC 94 605 2243 Antisense
GCCGTGTGCCAGTCGGTGGATTTTC 701 283 2258 Antisense
GACGGGACCACAACTATTCTGTATC 501 377 2298 Antisense
CCACGGTCGCATACGTGCTTCAATC 262 273 2350 Antisense
TGCTTCAATCGACTCGACTTGCCGC 465 579 2365 Antisense
GCCGCCATACAGAAGCTACGATCAG 16 287 2385 Antisense
Sequence Source Affymetrix Proprietary Database