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[187160_at]

Description C32A3.2 /REP_DB=WormBase Gene ID /WP=CE01506 /TR=SW:Q09261 /GB=CAA88285.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 55      4grp_total > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 83
1 2 3 4 range
A 12.7
(-1.2)
A 		6.3
(-2.2)
A 		1.5
(-3)
A 		39.1
(3)
A 		38
B 13.2
(0.2)
A 		2.3
(-3)
A 		12.8
(0.1)
A 		22.5
(3)
A 		20
C 39.9
(-0.3)
A 		2.4
(-3)
A 		4.5
(-2.8)
A 		84.4
(3)
P 		82
avr 21.9
(-0.6) 		3.7
(-3) 		6.3
(-2.7) 		48.7
(3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 31
1 2 3 4 5 6 range
A 3.8
(-2.6)
A 		2
(-3)
A 		32.1
(3)
A 		4.6
(-2.5)
A 		6
(-2.2)
A 		22.7
(1.1)
A 		30
B 7
(-1.7)
A 		26
(3)
A 		9.5
(-1.1)
A 		1.8
(-3)
A 		4.7
(-2.3)
A 		5.2
(-2.2)
A 		24
C 11.2
(-0.1)
A 		7.4
(-1.2)
A 		5.1
(-1.9)
A 		17.5
(1.8)
A 		1.5
(-3)
A 		21.6
(3)
A 		20
avr 7.3
(-1.5) 		11.8
(0.7) 		15.6
(2.6) 		8
(-1.1) 		4.1
(-3) 		16.5
(3) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 4.6
(1.8)
A 		1.4
(-1.9)
A 		0.4
(-3)
A 		5.7
(3)
A 		5
B 4.2
(2.1)
A 		0.6
(-3)
A 		3
(0.4)
A 		4.8
(3)
A 		4
C 13.2
(2.6)
A 		0.7
(-3)
A 		1.6
(-2.6)
A 		14.2
(3)
P 		14
avr 7.3
(2.3) 		0.9
(-3) 		1.7
(-2.3) 		8.2
(3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 0.7
(-3)
A 		0.7
(-3)
A 		7
(3)
A 		1.2
(-2.5)
A 		1.8
(-2)
A 		5.5
(1.6)
A 		6
B 2.4
(-1.6)
A 		8.6
(3)
A 		2.3
(-1.7)
A 		0.5
(-3)
A 		1.9
(-2)
A 		1.9
(-2)
A 		8
C 4.1
(0.2)
A 		2.3
(-1.4)
A 		1.3
(-2.3)
A 		3.5
(-0.3)
A 		0.5
(-3)
A 		7.2
(3)
A 		7
avr 2.4
(-1.3) 		3.9
(1.3) 		3.5
(0.6) 		1.7
(-2.5) 		1.4
(-3) 		4.9
(3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187160_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6676
Sequence Type Exemplar sequence
Representative Public ID C32A3.2 NCBI
Target Description C32A3.2 /REP_DB=WormBase Gene ID /WP=CE01506 /TR=SW:Q09261 /GB=CAA88285.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065335(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065335 NCBI Caenorhabditis elegans C32A3.2 (C32A3.2) mRNA, complete cds. 11/11 None
GENEFINDER00000010777 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3613284:3630345:-1 11/11 None
SNAP00000010769 ENSEMBL cdna:SNAP chromosome:CEL140:III:3624850:3630345:-1 11/11 None
C32A3.2 ENSEMBL cdna:known chromosome:CEL140:III:3628338:3630539:-1 gene:C32A3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3628337-3630539(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl C32A3.2
Entrez Gene 183104 Entrez gene
SwissProt Q09261 EMBL-EBI
WormBase CE01506 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE64938 Hypothetical protein CBG09769 [Caenorhabditis briggsae] 1.0E-165
blast CAC42265 Hypothetical protein C32A3.1a [Caenorhabditis elegans] ref|NP_001021194.1| Suppressor/Enhancer of Lin-12 family member (sel-8) [Caenorhabditis elegans] gb|AAF71522.1| LAG-3A [Caenorhabditis elegans] sp|Q09260|LAG3_CAEEL Lag-3 protein (Abnormal germ line proliferation protein 3) (Abnormal cell lineage protein 3) 1.0E-165
blast CAE64938 Hypothetical protein CBG09769 [Caenorhabditis briggsae] 1.0E-156
blast CAA88285 Hypothetical protein C32A3.2 [Caenorhabditis elegans] ref|NP_497736.1| C32A3.2 [Caenorhabditis elegans] sp|Q09261|YQC2_CAEEL Hypothetical protein C32A3.2 in chromosome III 1.0E-153
blast CAA88284 Hypothetical protein C32A3.1b [Caenorhabditis elegans] ref|NP_001021195.1| Suppressor/Enhancer of Lin-12 family member (sel-8) [Caenorhabditis elegans] gb|AAF71523.1| LAG-3B [Caenorhabditis elegans] 1.0E-153
blast CAA88285 Hypothetical protein C32A3.2 [Caenorhabditis elegans] ref|NP_497736.1| C32A3.2 [Caenorhabditis elegans] sp|Q09261|YQC2_CAEEL Hypothetical protein C32A3.2 in chromosome III 1.0E-83
blast CAE64938 Hypothetical protein CBG09769 [Caenorhabditis briggsae] 1.0E-69
blast EAL30519 GA19216-PA [Drosophila pseudoobscura] 6.0E-32
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497736.1 1 29-48
C32A3.2 1 29-48
Sequence
Target Sequence 
>C. ELEGANS:187160_AT
gaaatgctcgatgatcaggcacttatcggacttcttgccgatgtggcgggcgatgccaaa
gttgagaagaagcttcctcccaaatccgctcaacttcgacaagaaaaacgaggacttgtt
cttctcagaaaagagattttctatcaggcagttcaaagtggcttcacgacagaagaagct
cgtgtcaaatcagagacaattgtcaatgaagcacagataaagctccaagagcagcgaaaa
gctcttctaaatgatgttagagaaaaggtggaacaagaagaagttgaagagacggagaga
agtgagaaggatcagaagttgttcacgatggccttggaatttatggaaaagatttacaag
gatgatttgattagtagtgctgttcaatttccaacggctcattcggaccaacaaattctt
tcgaagaacaaatcgaatggacaacaaaaggaaaacaatgggaatatccaatcgataatg
agttcaaaatgggcgatgaatcgaatgtttcattcattgatcacgtattcttggagagac
atttaccatcattgggtatcccgaaatctggtccaattgctcattttatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATGCTCGATGATCAGGCACTTA 12 363 424 Antisense
CAGGCACTTATCGGACTTCTTGCCG 338 209 439 Antisense
ACTTCTTGCCGATGTGGCGGGCGAT 628 101 453 Antisense
TGAGAAGAAGCTTCCTCCCAAATCC 435 569 486 Antisense
TATCAGGCAGTTCAAAGTGGCTTCA 147 657 565 Antisense
GAAGTTGTTCACGATGGCCTTGGAA 490 337 738 Antisense
TGCTGTTCAATTTCCAACGGCTCAT 562 581 801 Antisense
GGCTCATTCGGACCAACAAATTCTT 136 539 819 Antisense
CATTCATTGATCACGTATTCTTGGA 489 215 934 Antisense
TGGGTATCCCGAAATCTGGTCCAAT 618 551 976 Antisense
TCTGGTCCAATTGCTCATTTTATGC 359 607 990 Antisense
Sequence Source Affymetrix Proprietary Database