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[187155_at]

Description Y53G8AR.8 /REP_DB=WormBase Gene ID /WP=CE26168 /TR=Q9N3G1 /GB=AAF59541.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 80      6grp_high1_10_10 > 15      4grp_highest_8_8 > 32      4grp_high_8_8 > 51      6grp_highest_nonorm > 70      6grp_total > 05      4grp_total > 60      6grp_high1_sub > De      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 221
1 2 3 4 range
A 375.7
(3)
P 		375.5
(3)
P 		316.4
(-0.5)
P 		274.9
(-3)
P 		101
B 363.4
(3)
P 		328.4
(1.9)
P 		362.1
(3)
P 		171.1
(-3)
P 		192
C 380.4
(2.6)
P 		392.1
(3)
P 		385.3
(2.8)
P 		193.4
(-3)
P 		199
avr 373.2
(3) 		365.3
(2.7) 		354.6
(2.3) 		213.1
(-3) 		160

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 140
1 2 3 4 5 6 range
A 341
(1.5)
P 		376.4
(3)
P 		260.2
(-2)
P 		295.1
(-0.5)
P 		317.7
(0.5)
P 		237.9
(-3)
P 		139
B 353.8
(3)
P 		305.5
(0.5)
P 		242.4
(-2.7)
P 		236.4
(-3)
P 		319.5
(1.2)
P 		240.9
(-2.8)
P 		117
C 324.6
(3)
P 		312.5
(2.1)
P 		289.6
(0.5)
P 		239.9
(-3)
P 		250.7
(-2.2)
P 		245.9
(-2.6)
P 		85
avr 339.8
(3) 		331.5
(2.5) 		264.1
(-1.6) 		257.1
(-2.1) 		296
(0.3) 		241.6
(-3) 		98
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 104
1 2 3 4 range
A 136.8
(3)
P 		81.5
(-0.4)
P 		82.7
(-0.4)
P 		40.2
(-3)
P 		97
B 115.9
(3)
P 		81.1
(0.4)
P 		83.9
(0.6)
P 		36.5
(-3)
P 		79
C 125.9
(2.5)
P 		112.7
(1.7)
P 		134.3
(3)
P 		32.6
(-3)
P 		102
avr 126.2
(3) 		91.8
(0.7) 		100.3
(1.3) 		36.4
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 62.9
(-2.5)
P 		133.9
(3)
P 		56.7
(-3)
P 		74.8
(-1.6)
P 		95.9
(0)
P 		57.9
(-2.9)
P 		77
B 123.1
(2.4)
P 		101.7
(0.6)
P 		58.9
(-3)
P 		62.6
(-2.7)
P 		129.8
(3)
P 		89.8
(-0.4)
P 		71
C 120.3
(3)
P 		95.4
(0.9)
P 		73.7
(-0.9)
P 		48.2
(-3)
P 		81.1
(-0.3)
P 		82.3
(-0.2)
P 		72
avr 102.1
(2) 		110.3
(3) 		63.1
(-2.9) 		61.9
(-3) 		102.3
(2) 		76.7
(-1.2) 		48
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187155_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7203
Sequence Type Exemplar sequence
Representative Public ID Y53G8AR.8 NCBI
Target Description Y53G8AR.8 /REP_DB=WormBase Gene ID /WP=CE26168 /TR=Q9N3G1 /GB=AAF59541.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065285(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065285 NCBI Caenorhabditis elegans Y53G8AR.8 (Y53G8AR.8) mRNA, complete cds. 11/11 None
GENEFINDER00000011809 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3290244:3294318:-1 11/11 None
SNAP00000011801 ENSEMBL cdna:SNAP chromosome:CEL140:III:3290252:3294222:-1 11/11 None
Y53G8AR.8 ENSEMBL cdna:known chromosome:CEL140:III:3290244:3294321:-1 gene:Y53G8AR.8 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3291890-3294318(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y53G8AR.8
Entrez Gene 175431 Entrez gene
SwissProt Q9N3G1 EMBL-EBI
WormBase CE25420 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1623293_AT dm
DROSGENOME1:149144_AT dm
Protein Similarities
Method ID Description E-Value
blast AAF59541 Hypothetical protein Y53G8AR.8 [Caenorhabditis elegans] ref|NP_497686.2| Y53G8AR.8 [Caenorhabditis elegans] 0.0
blast CAE69480 Hypothetical protein CBG15682 [Caenorhabditis briggsae] 1.0E-175
blast CAE69480 Hypothetical protein CBG15682 [Caenorhabditis briggsae] 1.0E-174
blast AAF59541 Hypothetical protein Y53G8AR.8 [Caenorhabditis elegans] ref|NP_497686.2| Y53G8AR.8 [Caenorhabditis elegans] 1.0E-154
blast CAE69480 Hypothetical protein CBG15682 [Caenorhabditis briggsae] 2.0E-82
blast AAF51634 CG4042-PA [Drosophila melanogaster] ref|NP_649250.2| CG4042-PA [Drosophila melanogaster] 3.0E-24
Sequence
Target Sequence 
>C. ELEGANS:187155_AT
tcatcgaagcaatcgacgcctcgggcacccaaataatgttcgaagggcttgaaaatctgg
aaaatctccaaaaacttcggcttctccgcctggcgaacagcgaatatgtcgatgattggt
gtattggaaggatcggaggacttctaccgaatcttgaaatgcttgatctatccggatgtc
atcgaatctcatctaaaggcctaatgggcctaaaagcctcaaaaaatttgaaatttctac
gtctggaagggctctcgggcattcggaatctgggaaaatctgctctaatccttgaggatt
tactcccaaaacttcaaattcttggaatggattatgagcttagttttagacaaattgagg
ccgaaaataggcttttggagcaggaaagagtcgttcttgatggccgaggaaatgcttttg
tggaagatgataattctcggctttttctggttatgtctacgtcatcagatgataaagcgg
tcactgatgatcatgataacccgattatgaccagtactgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCATCGAAGCAATCGACGCCTCGGG 303 621 539 Antisense
ACGCCTCGGGCACCCAAATAATGTT 297 91 554 Antisense
CTTCTCCGCCTGGCGAACAGCGAAT 152 219 619 Antisense
GGAGGACTTCTACCGAATCTTGAAA 512 515 673 Antisense
GCTTGATCTATCCGGATGTCATCGA 450 307 699 Antisense
GTCATCGAATCTCATCTAAAGGCCT 656 465 716 Antisense
AATTTCTACGTCTGGAAGGGCTCTC 544 171 770 Antisense
GCTCTCGGGCATTCGGAATCTGGGA 109 301 789 Antisense
GATGATAATTCTCGGCTTTTTCTGG 202 411 964 Antisense
GGCTTTTTCTGGTTATGTCTACGTC 71 539 977 Antisense
TAACCCGATTATGACCAGTACTGTG 204 637 1035 Antisense
Sequence Source Affymetrix Proprietary Database