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[187153_s_at]

Description Y39A3CL.5 /REP_DB=WormBase Gene ID /WP=CE25258 /TR=Q9N414 /GB=AAF60521.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 27      4grp_highest_8_8 > 53      4grp_high_8_8 > 17      6grp_highest_nonorm > 0      6grp_total > 17      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 12      6grp_high1_sub > Cu      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1261
1 2 3 4 range
A 2101.3
(3)
P 		1147.3
(-2.3)
P 		1022.4
(-3)
P 		1797.6
(1.3)
P 		1079
B 2283.5
(3)
P 		1232.1
(-2.4)
P 		1113.6
(-3)
P 		1741.8
(0.2)
P 		1170
C 1878.3
(3)
P 		1242.3
(-1.8)
P 		1083.8
(-3)
P 		1514.1
(0.2)
P 		795
avr 2087.7
(3) 		1207.2
(-2.2) 		1073.3
(-3) 		1684.5
(0.6) 		1014

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2250
1 2 3 4 5 6 range
A 3114.8
(3)
P 		1523.8
(-1.2)
P 		1031.9
(-2.6)
P 		864.9
(-3)
P 		1119.6
(-2.3)
P 		1436.5
(-1.5)
P 		2250
B 2879.6
(3)
P 		1792.9
(-0.4)
P 		998.3
(-2.8)
P 		1366.4
(-1.7)
P 		934
(-3)
P 		1435.8
(-1.5)
P 		1946
C 2202.8
(3)
P 		1586.4
(0.1)
P 		1230.8
(-1.6)
P 		925.9
(-3)
P 		951.8
(-2.9)
P 		1272.4
(-1.4)
P 		1277
avr 2732.4
(3) 		1634.4
(-0.8) 		1087
(-2.7) 		1052.4
(-2.8) 		1001.8
(-3) 		1381.6
(-1.7) 		1731
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 516
1 2 3 4 range
A 765.1
(3)
P 		249
(-3)
P 		267.3
(-2.8)
P 		263.1
(-2.8)
P 		516
B 728.2
(3)
P 		304.3
(-2.4)
P 		258
(-3)
P 		371.2
(-1.6)
P 		470
C 621.8
(3)
P 		357.2
(-1.3)
P 		377.7
(-1)
P 		255.3
(-3)
P 		367
avr 705
(3) 		303.5
(-2.9) 		301
(-2.9) 		296.5
(-3) 		409

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 816
1 2 3 4 5 6 range
A 574.2
(3)
P 		541.9
(2.5)
P 		224.7
(-2.9)
P 		219.4
(-3)
P 		337.8
(-1)
P 		349.7
(-0.8)
P 		355
B 1002
(3)
P 		596.8
(-0.2)
P 		242.4
(-3)
P 		361.9
(-2.1)
P 		379.5
(-1.9)
P 		535.4
(-0.7)
P 		760
C 816.5
(3)
P 		484.1
(-0.2)
P 		313.3
(-1.8)
P 		186.2
(-3)
P 		308
(-1.8)
P 		426
(-0.7)
P 		630
avr 797.6
(3) 		540.9
(0.2) 		260.1
(-3) 		255.8
(-3) 		341.8
(-2) 		437
(-1) 		542
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187153_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8145
Sequence Type Exemplar sequence
Representative Public ID Y39A3CL.5 NCBI
Target Description Y39A3CL.5 /REP_DB=WormBase Gene ID /WP=CE25258 /TR=Q9N414 /GB=AAF60521.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_181914(11), NM_065059(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_181914 NCBI Caenorhabditis elegans CaLPain family member (clp-4) (clp-4) mRNA, complete cds. 11/11 None
NM_065059 NCBI Caenorhabditis elegans CaLPain family member (clp-4) (clp-4) mRNA, complete cds. 11/11 None
SNAP00000009083 ENSEMBL cdna:SNAP chromosome:CEL140:III:1804588:1814794:-1 11/11 None
GENEFINDER00000009087 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1807267:1816079:-1 11/11 None
Y39A3CL.5b.1 ENSEMBL cdna:known chromosome:CEL140:III:1807729:1818314:-1 gene:Y39A3CL.5 11/11 None
Y39A3CL.5b.2 ENSEMBL cdna:known chromosome:CEL140:III:1807729:1818312:-1 gene:Y39A3CL.5 11/11 None
Y39A3CL.5a ENSEMBL cdna:known chromosome:CEL140:III:1807735:1818308:-1 gene:Y39A3CL.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1807879-1818308(-) 99.66 100.0
Public Domain and Genome References
Ensembl Y39A3CL.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO12403 Calpain family protein 4, isoform b [Caenorhabditis elegans] ref|NP_871643.1| CaLPain family member (clp-4) [Caenorhabditis elegans] 0.0
blast AAO12402 Calpain family protein 4, isoform a [Caenorhabditis elegans] ref|NP_497460.2| CaLPain family member (clp-4) [Caenorhabditis elegans] 0.0
blast CAE69108 Hypothetical protein CBG15134 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.5E-112
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.7E-51
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 8.0E-49
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.5E-112
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.7E-51
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 8.0E-49
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 4.5E-42
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 7.8E-58
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.8E-51
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 3.3E-49
Sequence
Target Sequence 
>C. ELEGANS:187153_S_AT
gtcttttgaggatttcttctccaactttacacaaatggaagtttgtaatctgactgcgga
gattttcgatgaaatcgctgaaatgactggcgtcaacagagcaactgagacagtcgagga
ggaacatcagtggcatgagatcatggaggacggggaatggagttctaaaaaaggcacagc
tggaggctgcaacaacaacccatctacctaccccaagaaccctcaattctccactttctt
cacagcaccacaatcctctattgaagccgacgggaatgttaccgtaatcgttgcagttct
acaaaagtacagaagagagctccgttcaaagggcaaagatgtcctgccaattggtgtatc
gatctactcacttggcgccgaaggcaccgcccggagccctctaaccgcccagttcttctc
gcaaaaccgcccgatcgcccgaactacagtattcgtgaatactagagaggttactgtaag
attccgtgtaccacctgggcaatatgtcattgtcccatgtacctttgatgcttacgatga
tgccgagtttctgctcagagtctat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTTTTGAGGATTTCTTCTCCAAC 421 469 1464 Antisense
TGGAGGCTGCAACAACAACCCATCT 64 551 1644 Antisense
GCACCACAATCCTCTATTGAAGCCG 523 313 1708 Antisense
GTTACCGTAATCGTTGCAGTTCTAC 60 447 1741 Antisense
AAGAGAGCTCCGTTCAAAGGGCAAA 278 151 1776 Antisense
GATGTCCTGCCAATTGGTGTATCGA 158 409 1801 Antisense
TGGTGTATCGATCTACTCACTTGGC 450 553 1815 Antisense
GATCGCCCGAACTACAGTATTCGTG 340 417 1896 Antisense
TCCGTGTACCACCTGGGCAATATGT 302 595 1946 Antisense
TGTCATTGTCCCATGTACCTTTGAT 387 559 1968 Antisense
TGCCGAGTTTCTGCTCAGAGTCTAT 501 585 2004 Antisense
Sequence Source Affymetrix Proprietary Database