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[187151_s_at]

Description F54C8.7 /REP_DB=WormBase Gene ID /WP=CE00193 /TR=SW:P34445 /GB=CAA80157.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 20      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 73      6grp_total > 08      4grp_total > 50      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 313
1 2 3 4 range
A 453.2
(-2.3)
P 		669.7
(3)
P 		496.3
(-1.2)
P 		424.2
(-3)
P 		246
B 464.5
(-0.2)
P 		557.3
(2.2)
P 		589.8
(3)
P 		356.6
(-3)
P 		233
C 465.2
(-1.5)
P 		653.2
(3)
P 		560.4
(0.8)
P 		400.6
(-3)
P 		253
avr 461
(-1.3) 		626.7
(3) 		548.8
(1) 		393.8
(-3) 		233

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 441
1 2 3 4 5 6 range
A 428.7
(-1)
P 		453.6
(-0.6)
P 		540.4
(1)
P 		651.4
(3)
P 		320.2
(-3)
P 		544.8
(1.1)
P 		331
B 489.4
(3)
P 		445.7
(-0.1)
P 		483.5
(2.6)
P 		404.9
(-3)
P 		447.3
(0)
P 		467.3
(1.4)
P 		85
C 761.4
(3)
P 		435.6
(-2.9)
P 		604.6
(0.2)
P 		469.3
(-2.3)
P 		487.9
(-2)
P 		430.4
(-3)
P 		331
avr 559.8
(3) 		445
(-1.9) 		542.8
(2.3) 		508.5
(0.8) 		418.5
(-3) 		480.8
(-0.4) 		141
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 133
1 2 3 4 range
A 165
(3)
P 		145.4
(1.9)
P 		129.8
(0.9)
P 		62.1
(-3)
P 		103
B 148.1
(3)
P 		137.6
(2.1)
P 		136.7
(2.1)
P 		76
(-3)
P 		72
C 154
(1.1)
P 		187.8
(2.6)
P 		195.3
(3)
P 		67.5
(-3)
P 		128
avr 155.7
(2.9) 		156.9
(3) 		153.9
(2.8) 		68.5
(-3) 		88

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 203
1 2 3 4 5 6 range
A 79
(-3)
P 		161.3
(2.7)
P 		117.7
(-0.3)
P 		165.2
(3)
P 		96.6
(-1.8)
P 		132.7
(0.7)
P 		86
B 170.3
(2.1)
P 		148.3
(0.3)
P 		117.4
(-2.2)
P 		107.2
(-3)
P 		181.7
(3)
P 		174.2
(2.4)
P 		75
C 282.2
(3)
P 		132.9
(-1.8)
P 		153.9
(-1.1)
P 		94.4
(-3)
P 		157.9
(-1)
P 		144.1
(-1.4)
P 		188
avr 177.2
(3) 		147.5
(-0.2) 		129.7
(-2.2) 		122.3
(-3) 		145.4
(-0.5) 		150.3
(0.1) 		55
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187151_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6049
Sequence Type Exemplar sequence
Representative Public ID F54C8.7 NCBI
Target Description F54C8.7 /REP_DB=WormBase Gene ID /WP=CE00193 /TR=SW:P34445 /GB=CAA80157.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027433(9), NM_181932(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027433 NCBI Caenorhabditis elegans F54C8.7a (F54C8.7) mRNA, complete cds. 9/11 None
NM_181932 NCBI Caenorhabditis elegans F54C8.7b (F54C8.7) mRNA, complete cds. 9/11 None
SNAP00000014865 ENSEMBL cdna:SNAP chromosome:CEL140:III:9451738:9453958:-1 11/11 None
GENEFINDER00000014869 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9451738:9453955:-1 10/11 None
F54C8.7a ENSEMBL cdna:known chromosome:CEL140:III:9451494:9452909:-1 gene:F54C8.7 9/11 A 
F54C8.7b ENSEMBL cdna:known chromosome:CEL140:III:9451738:9453958:-1 gene:F54C8.7 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9451733-9452905(-) 94.95 100.0
Public Domain and Genome References
Ensembl F54C8.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA80157 Hypothetical protein F54C8.7a [Caenorhabditis elegans] ref|NP_001022604.1| F54C8.7a [Caenorhabditis elegans] sp|P34445|YL87_CAEEL Hypothetical protein F54C8.7 in chromosome III 1.0E-146
blast CAD82913 Hypothetical protein F54C8.7b [Caenorhabditis elegans] ref|NP_871661.1| F54C8.7b [Caenorhabditis elegans] 1.0E-143
blast CAD82913 Hypothetical protein F54C8.7b [Caenorhabditis elegans] ref|NP_871661.1| F54C8.7b [Caenorhabditis elegans] 1.0E-139
blast CAD82913 Hypothetical protein F54C8.7b [Caenorhabditis elegans] ref|NP_871661.1| F54C8.7b [Caenorhabditis elegans] 1.0E-137
blast CAE65214 Hypothetical protein CBG10089 [Caenorhabditis briggsae] 1.0E-128
blast CAD82913 Hypothetical protein F54C8.7b [Caenorhabditis elegans] ref|NP_871661.1| F54C8.7b [Caenorhabditis elegans] 1.0E-125
blast CAA80157 Hypothetical protein F54C8.7a [Caenorhabditis elegans] ref|NP_001022604.1| F54C8.7a [Caenorhabditis elegans] sp|P34445|YL87_CAEEL Hypothetical protein F54C8.7 in chromosome III 1.0E-125
Protein Domains
Database ID Description E-Value
Pfam IPR010504 EMBL-EBI Arfaptin-like 2.4E-41
Pfam IPR010504 EMBL-EBI Arfaptin-like 6.1E-66
Pfam IPR010504 EMBL-EBI Arfaptin-like 2.4E-41
Pfam IPR010504 EMBL-EBI Arfaptin-like 4.0E-66
Pfam IPR010504 EMBL-EBI Arfaptin-like 9.6E-117
Pfam IPR010504 EMBL-EBI Arfaptin-like 9.6E-117
Sequence
Target Sequence 
>C. ELEGANS:187151_S_AT
gtcatgttctcactcaaatggctgaagctgagaagaaactttcggaatcattttatcaat
tgtcaatgaaagaagaacagattaaggcaagtttcaaaaaaaaaccacttgaaatcgagt
ttcaagcgcgctccatggccagaagttgggcacaatgcaccatgacatcagaaactatga
gaggagttggagagcaggctgcctcattggatgcatgcttgagatacttcatttcttcta
tggaaactgtctataaccaaacaattactgacactttacacacaatttataatactgaat
cggctcgcattgagtatgatgttgaccgaaatgatatctcagctgcaactaatcctccac
aaggacaactgacgaagaatctcccagtcggtgcaaccgaaaagtgtgaagagaaaaaag
caaagtatgagaagctaaagaatgatgcgaagatcaaaatgcgattgcttgaagagaatc
gaatctcggtggtcgcagctcaactcgagaaactccaatcagcactcgctgcatactatt
cgggaaatgcgaagctcctggaagcatcggttcgcgagctgagctt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCATGTTCTCACTCAAATGGCTGA 76 467 317 Antisense
GAAATCGAGTTTCAAGCGCGCTCCA 242 361 427 Antisense
CGCGCTCCATGGCCAGAAGTTGGGC 95 257 443 Antisense
GCAGGCTGCCTCATTGGATGCATGC 26 311 510 Antisense
TATCTCAGCTGCAACTAATCCTCCA 89 659 651 Antisense
AGAATCGAATCTCGGTGGTCGCAGC 628 73 791 Antisense
GTCGCAGCTCAACTCGAGAAACTCC 329 475 808 Antisense
AAACTCCAATCAGCACTCGCTGCAT 208 127 826 Antisense
CACTCGCTGCATACTATTCGGGAAA 197 199 839 Antisense
GCGAAGCTCCTGGAAGCATCGGTTC 100 289 865 Antisense
AAGCATCGGTTCGCGAGCTGAGCTT 520 149 878 Antisense
Sequence Source Affymetrix Proprietary Database