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[187116_s_at]

Description B0513.5 /REP_DB=WormBase Gene ID /WP=CE15561 /TR=SW:O45228 /GB=CAB05117.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 12      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 40      6grp_total > 23      4grp_total > 55      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 867
1 2 3 4 range
A 929.6
(3)
P 		469.8
(-1.3)
P 		387.3
(-2.1)
P 		292.7
(-3)
P 		637
B 1005.7
(3)
P 		360.1
(-2)
P 		432.6
(-1.5)
P 		238.6
(-3)
P 		767
C 1106
(3)
P 		391.2
(-2.2)
P 		476
(-1.6)
P 		277.7
(-3)
P 		828
avr 1013.8
(3) 		407
(-1.9) 		432
(-1.7) 		269.7
(-3) 		744

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 635
1 2 3 4 5 6 range
A 807.2
(3)
P 		739.2
(2.3)
P 		699.6
(1.8)
P 		490.1
(-0.4)
P 		486.4
(-0.4)
P 		248
(-3)
P 		559
B 829.5
(3)
P 		829.5
(3)
P 		761.3
(2.3)
P 		491.3
(-0.5)
P 		425.3
(-1.2)
P 		249.8
(-3)
P 		580
C 871.8
(3)
P 		801.2
(2.3)
P 		545.3
(-0.1)
P 		525.9
(-0.3)
P 		363.8
(-1.8)
P 		236.5
(-3)
P 		635
avr 836.2
(3) 		790
(2.5) 		668.7
(1.3) 		502.4
(-0.4) 		425.2
(-1.2) 		244.8
(-3) 		591
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 323
1 2 3 4 range
A 338.5
(3)
P 		102
(-1.8)
P 		101.2
(-1.8)
P 		42.8
(-3)
P 		296
B 320.7
(3)
P 		88.9
(-2.2)
P 		100.2
(-1.9)
P 		50.9
(-3)
P 		270
C 366.1
(3)
P 		112.5
(-1.8)
P 		165.9
(-0.8)
P 		46.8
(-3)
P 		319
avr 341.8
(3) 		101.1
(-1.9) 		122.4
(-1.5) 		46.8
(-3) 		295

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 263
1 2 3 4 5 6 range
A 148.8
(-0.4)
P 		262.9
(3)
P 		152.4
(-0.3)
P 		124.3
(-1.1)
P 		146.8
(-0.4)
P 		60.4
(-3)
P 		203
B 288.6
(3)
P 		276.1
(2.6)
P 		184.9
(-0.2)
P 		130.1
(-1.9)
P 		172.8
(-0.6)
P 		93.1
(-3)
P 		196
C 323.1
(3)
P 		244.5
(1.1)
P 		138.8
(-1.5)
P 		105.8
(-2.3)
P 		117.7
(-2.1)
P 		79.2
(-3)
P 		244
avr 253.5
(2.7) 		261.2
(3) 		158.7
(-0.3) 		120.1
(-1.6) 		145.8
(-0.8) 		77.6
(-3) 		184
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187116_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8899
Sequence Type Exemplar sequence
Representative Public ID B0513.5 NCBI
Target Description B0513.5 /REP_DB=WormBase Gene ID /WP=CE15561 /TR=SW:O45228 /GB=CAB05117.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070268(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070268 NCBI Caenorhabditis elegans B0513.5 (B0513.5) mRNA, complete cds. 11/11 None
SNAP00000026159 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13868757:13876574:-1 11/11 None
GENEFINDER00000026164 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13868757:13876574:-1 11/11 None
B0513.5 ENSEMBL cdna:known chromosome:CEL140:IV:13868542:13876584:-1 gene:B0513.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13868762-13874307(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl B0513.5
Entrez Gene 178350 Entrez gene
SwissProt O45228 EMBL-EBI
WormBase CE37309 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.21840.1.S1_AT similar to proline dehydrogenase (oxidase) 1 cfa
DROSOPHILA_2:1640090_A_AT sluggish A dm
DROSGENOME1:151627_S_AT sluggish A dm
DROSGENOME1:142569_AT sluggish A dm
HG-U133_PLUS_2:214203_S_AT proline dehydrogenase (oxidase) 1 hs
HG-U133A_2:214203_S_AT proline dehydrogenase (oxidase) 1 hs
HG-U133A:214203_S_AT proline dehydrogenase (oxidase) 1 hs
HU35KSUBB:RC_AA076336_AT proline dehydrogenase (oxidase) 1 hs
U133_X3P:HS.274550.0.S1_3P_S_AT proline dehydrogenase (oxidase) 1 hs
HG-U95AV2:34109_AT proline dehydrogenase (oxidase) 1 hs
HG-U95AV2:34110_G_AT proline dehydrogenase (oxidase) 1 hs
HG-U95AV2:40042_R_AT proline dehydrogenase (oxidase) 1 hs
HG-U95E:67150_AT Proline dehydrogenase (oxidase) 1 hs
HG-U95E:67152_G_AT Proline dehydrogenase (oxidase) 1 hs
HG-U95D:69997_AT Similar to proline dehydrogenase (oxidase) 1; tumor protein p53 inducible protein 6; p53 induced protein; proline oxidase 2; proline dehydrogenase (proline oxidase ) hs
MG-U74BV2:165239_F_AT proline dehydrogenase mm
MG-U74BV2:162728_AT proline dehydrogenase mm
MOE430A:1417629_AT proline dehydrogenase mm
MOUSE430A_2:1417629_AT proline dehydrogenase mm
MOUSE430_2:1417629_AT proline dehydrogenase mm
MU19KSUBB:TC26282_AT Brain and kidney proline oxidase 2 (MmPOX2) mm
MU19KSUBB:TC28728_AT Brain and kidney proline oxidase 2 (MmPOX2) mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6537 glutamate biosynthesis inferred from electronic annotation QuickGO AmiGO
6562 proline catabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4657 proline dehydrogenase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05117 Hypothetical protein B0513.5 [Caenorhabditis elegans] ref|NP_502669.2| B0513.5 [Caenorhabditis elegans] sp|O45228|PROD_CAEEL Probable proline oxidase, mitochondrial precursor (Proline dehydrogenase) 0.0
blast CAE74609 Hypothetical protein CBG22398 [Caenorhabditis briggsae] 0.0
blast AAH97700 Unknown (protein for MGC:115247) [Xenopus laevis] 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR002872 EMBL-EBI Proline dehydrogenase 1.0E-126
Pfam IPR002872 EMBL-EBI Proline dehydrogenase 2.4E-102
Pfam IPR002872 EMBL-EBI Proline dehydrogenase 1.7E-59
Pfam IPR002872 EMBL-EBI Proline dehydrogenase 2.4E-102
Pfam IPR002872 EMBL-EBI Proline dehydrogenase 1.7E-59
Sequence
Target Sequence 
>C. ELEGANS:187116_S_AT
gtggaaacatcttcaacacctaccaagcatacttgaagggaacccttcaaaatatggagg
ctgatatgcaagttgccagacgtgaaggatggcatttcggtgcaaaacttgttcgtggag
catacatggagcaggagagagcacgagcaaaagcaattggttatgaggatccgattaatg
ataattttgaggccacctcaaaaatgtacgagtcatgtctgacacgtatcgccgatgaag
tacatcgtcgtggtaagaccaatgtgtccgtaatggtggcaagtcacaatgaggatacag
taagattcgcattgaatttgatgaaggaaaagtgtatttcaccatcggaacgtgttatgt
gcatggcacagttgtacggaatgtgtgatcaggtatcattctccctgggtcaagccggtt
tctcagtctacaaatatcttccatatggaccagttgaagaagttctaccatatctaagtc
gacgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGGAAACATCTTCAACACCTACCA 201 489 1103 Antisense
GGAGGCTGATATGCAAGTTGCCAGA 519 515 1158 Antisense
GACGTGAAGGATGGCATTTCGGTGC 446 377 1181 Antisense
TCGGTGCAAAACTTGTTCGTGGAGC 683 599 1199 Antisense
ATGTCTGACACGTATCGCCGATGAA 68 49 1317 Antisense
ATGTGTCCGTAATGGTGGCAAGTCA 555 49 1364 Antisense
GTGTATTTCACCATCGGAACGTGTT 32 487 1434 Antisense
TGTGCATGGCACAGTTGTACGGAAT 141 555 1460 Antisense
GATCAGGTATCATTCTCCCTGGGTC 250 419 1489 Antisense
AGCCGGTTTCTCAGTCTACAAATAT 463 83 1515 Antisense
AGTTCTACCATATCTAAGTCGACGT 118 65 1563 Antisense
Sequence Source Affymetrix Proprietary Database