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[187099_s_at]

Description C28C12.10 /REP_DB=WormBase Gene ID /WP=CE27740 /TR=Q18284 /GB=AAB37553.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 26      4grp_highest_8_8 > 53      4grp_high_8_8 > 16      6grp_highest_nonorm > 51      6grp_total > 36      4grp_total > 31      6grp_highest_sub > Cu      6grp_cgc > 25      6grp_high1_sub > De      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 418
1 2 3 4 range
A 800.3
(3)
P 		397.4
(-3)
P 		423.7
(-2.6)
P 		603.3
(0.1)
P 		403
B 760.7
(3)
P 		430
(-2.2)
P 		382.6
(-3)
P 		658.5
(1.4)
P 		378
C 796.4
(3)
P 		426
(-3)
P 		480.9
(-2.1)
P 		599.2
(-0.2)
P 		370
avr 785.8
(3) 		417.8
(-3) 		429.1
(-2.8) 		620.3
(0.3) 		368

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 420
1 2 3 4 5 6 range
A 614.6
(0.1)
P 		818.1
(3)
P 		398.4
(-3)
P 		660.1
(0.7)
P 		404.3
(-2.9)
P 		570.7
(-0.5)
P 		420
B 765.3
(3)
P 		596.7
(-0.1)
P 		572.1
(-0.5)
P 		488.3
(-2)
P 		435
(-3)
P 		585
(-0.3)
P 		330
C 739.8
(3)
P 		578.7
(-1.3)
P 		611.4
(-0.4)
P 		525.9
(-2.7)
P 		514.6
(-3)
P 		534.2
(-2.5)
P 		225
avr 706.6
(3) 		664.5
(2) 		527.3
(-1.2) 		558.1
(-0.5) 		451.3
(-3) 		563.3
(-0.4) 		255
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 205
1 2 3 4 range
A 291.4
(3)
P 		86.3
(-3)
P 		110.8
(-2.3)
P 		88.3
(-2.9)
P 		205
B 242.6
(3)
P 		106.2
(-2.3)
P 		88.7
(-3)
P 		140.3
(-1)
P 		154
C 263.6
(3)
P 		122.5
(-2.2)
P 		167.6
(-0.5)
P 		101
(-3)
P 		163
avr 265.9
(3) 		105
(-3) 		122.4
(-2.4) 		109.9
(-2.8) 		161

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 204
1 2 3 4 5 6 range
A 113.3
(-2.2)
P 		291
(3)
P 		86.8
(-3)
P 		167.4
(-0.6)
P 		122
(-2)
P 		138.9
(-1.5)
P 		204
B 266.3
(3)
P 		198.6
(0)
P 		138.9
(-2.6)
P 		129.3
(-3)
P 		176.7
(-0.9)
P 		218.2
(0.9)
P 		137
C 274.2
(3)
P 		176.6
(-0.5)
P 		155.6
(-1.2)
P 		105.8
(-3)
P 		166.5
(-0.8)
P 		178.8
(-0.4)
P 		168
avr 217.9
(2.7) 		222.1
(3) 		127.1
(-3) 		134.2
(-2.6) 		155.1
(-1.2) 		178.6
(0.3) 		95
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187099_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10003
Sequence Type Exemplar sequence
Representative Public ID C28C12.10 NCBI
Target Description C28C12.10 /REP_DB=WormBase Gene ID /WP=CE27740 /TR=Q18284 /GB=AAB37553.2 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069054(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069054 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-77) (tag-77) mRNA, complete cds. 11/11 None
C28C12.10 ENSEMBL cdna:known chromosome:CEL140:IV:8503475:8510467:-1 gene:C28C12.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8503661-8507461(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol tag-77
Ensembl C28C12.10
Entrez Gene 177656 Entrez gene
SwissProt Q18284 EMBL-EBI
WormBase CE27740 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37553 Temporarily assigned gene name protein 77 [Caenorhabditis elegans] ref|NP_501455.1| Temporarily Assigned Gene name family member (tag-77) [Caenorhabditis elegans] 0.0
blast CAE69442 Hypothetical protein CBG15630 [Caenorhabditis briggsae] 0.0
blast XP_854794 PREDICTED: similar to FYVE, RhoGEF and PH domain containing protein 6 (Zinc finger FYVE domain containing protein 24) [Canis familiaris] 7.0E-29
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.1E-16
Pfam IPR000306 EMBL-EBI Zn-finger, FYVE type 1.1E-27
Sequence
Target Sequence 
>C. ELEGANS:187099_S_AT
ggcaagctccactgtcgaaatatgactttacgaaacaaaatgtttgctccgagtgttttg
atcggcattacaaagcttacaaagacggtgttctattcccatctaaaaatatgatagtcc
aaagtgatgaaactattcttgtgaaaattggaaaacgcaacgacaaagaaattgtggatc
caagaaaattgttcaaggcacccgtcaattatggattccggcatcgaaatgttgaggaaa
aacgggcacagagcattgtgtttggcagggtttatttgagaagtcgaaaaaccgagactg
tccgccatgctcttcttcgtcgtgatgatcttaaactggtattctacaaagcagaacttg
actcaaaatctgttctagaattgctcatttatggatatttctaccgggaaacaccacttg
atgatggatggttatttgagcttgtacatcggaatcaaataagaactgatgataccaaag
atgacgtcatttccttccgtgtcgacaacagtgcaagtgcaaaaaagtggtcggcagcat
ttgcggat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGCAAGCTCCACTGTCGAAATATGA 668 533 2216 Antisense
ATGTTTGCTCCGAGTGTTTTGATCG 704 47 2255 Antisense
CAAAGACGGTGTTCTATTCCCATCT 656 191 2295 Antisense
GAAAATTGTTCAAGGCACCCGTCAA 365 353 2399 Antisense
GCACCCGTCAATTATGGATTCCGGC 559 315 2413 Antisense
CAGAGCATTGTGTTTGGCAGGGTTT 423 205 2464 Antisense
ATGCTCTTCTTCGTCGTGATGATCT 426 43 2522 Antisense
TTCTACCGGGAAACACCACTTGATG 5 689 2614 Antisense
AAAGATGACGTCATTTCCTTCCGTG 190 121 2692 Antisense
TCCTTCCGTGTCGACAACAGTGCAA 372 591 2707 Antisense
AAAGTGGTCGGCAGCATTTGCGGAT 136 121 2739 Antisense
Sequence Source Affymetrix Proprietary Database