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[187093_at]

Description Y65B4BR.5 /REP_DB=WormBase Gene ID /WP=CE22740 /TR=Q9N2Z8 /GB=AAF60854.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 43      4grp_highest_8_8 > 22      4grp_high_8_8 > 14      6grp_highest_nonorm > 18      6grp_total > 20      4grp_total > 09      6grp_highest_sub > 02_03      6grp_high1_sub > 02      6grp_total_sub > Hat      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29840
1 2 3 4 range
A 18777.6
(-3)
P 		22334.8
(-2.2)
P 		22958.2
(-2.1)
P 		46377.3
(3)
P 		27600
B 18869.8
(-3)
P 		19058.6
(-2.9)
P 		21669.7
(-2.1)
P 		38042.6
(3)
P 		19173
C 16537.2
(-3)
P 		18003
(-2.7)
P 		18200.6
(-2.6)
P 		42737.9
(3)
P 		26201
avr 18061.5
(-3) 		19798.8
(-2.6) 		20942.8
(-2.3) 		42385.9
(3) 		24324

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7187
1 2 3 4 5 6 range
A 14257.6
(-1.5)
P 		17658.3
(2.7)
P 		15984.6
(0.7)
P 		17871.8
(3)
P 		13047.4
(-3)
P 		16932.3
(1.8)
P 		4824
B 16068.6
(1.5)
P 		17121.6
(3)
P 		14591.4
(-0.6)
P 		13586.9
(-2)
P 		12898.7
(-3)
P 		13115.6
(-2.7)
P 		4223
C 14509.3
(-0.8)
P 		17265.5
(1.5)
P 		19031.8
(3)
P 		14250
(-1)
P 		14921.7
(-0.4)
P 		11844.4
(-3)
P 		7187
avr 14945.2
(-0.9) 		17348.5
(3) 		16535.9
(1.7) 		15236.2
(-0.4) 		13622.6
(-3) 		13964.1
(-2.5) 		3726
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3400
1 2 3 4 range
A 6836.9
(3)
P 		4848.3
(-3)
P 		6001.7
(0.5)
P 		6788.7
(2.9)
P 		1989
B 6017.4
(-0.7)
P 		4707
(-3)
P 		5021.4
(-2.4)
P 		8106.9
(3)
P 		3400
C 5474.3
(-2.1)
P 		5176
(-3)
P 		6343.5
(0.5)
P 		7206.3
(3)
P 		2030
avr 6109.5
(-0.1) 		4910.4
(-3) 		5788.9
(-0.9) 		7367.3
(3) 		2457

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3652
1 2 3 4 5 6 range
A 2628.3
(-3)
P 		6280.1
(3)
P 		3481.1
(-1.6)
P 		4532.7
(0.1)
P 		3937.1
(-0.8)
P 		4122.5
(-0.5)
P 		3652
B 5591.1
(2.7)
P 		5699
(3)
P 		3543.2
(-3)
P 		3598.7
(-2.8)
P 		5240.6
(1.7)
P 		4890.7
(0.8)
P 		2156
C 5378
(3)
P 		5268.2
(2.7)
P 		4843.9
(1.7)
P 		2865.7
(-3)
P 		4828.5
(1.7)
P 		3965
(-0.4)
P 		2512
avr 4532.5
(-0.5) 		5749.1
(3) 		3956.1
(-2.2) 		3665.7
(-3) 		4668.7
(-0.1) 		4326.1
(-1.1) 		2083
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187093_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2550
Sequence Type Exemplar sequence
Representative Public ID Y65B4BR.5 NCBI
Target Description Y65B4BR.5 /REP_DB=WormBase Gene ID /WP=CE22740 /TR=Q9N2Z8 /GB=AAF60854.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058348(11), NM_182046(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058348 NCBI Caenorhabditis elegans Y65B4BR.5a (Y65B4BR.5) mRNA, complete cds. 11/11 A 
NM_182046 NCBI Caenorhabditis elegans Y65B4BR.5b (Y65B4BR.5) mRNA, complete cds. 11/11 A 
SNAP00000036979 ENSEMBL cdna:SNAP chromosome:CEL140:I:535811:536496:1 11/11 None
GENEFINDER00000036995 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:535811:541634:1 11/11 None
Y65B4BR.5a.3 ENSEMBL cdna:known chromosome:CEL140:I:535788:536588:1 gene:Y65B4BR.5 11/11 None
Y65B4BR.5a.2 ENSEMBL cdna:known chromosome:CEL140:I:535788:536881:1 gene:Y65B4BR.5 11/11 A 
Y65B4BR.5b ENSEMBL cdna:known chromosome:CEL140:I:535792:536619:1 gene:Y65B4BR.5 11/11 A 
Y65B4BR.5a.1 ENSEMBL cdna:known chromosome:CEL140:I:535794:536588:1 gene:Y65B4BR.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:535810-536496(+) 100.0 100.0
Public Domain and Genome References
Ensembl Y65B4BR.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN73850 Ww domain protein (e3 ubiquitin ligase) protein 1, isoform b [Caenorhabditis elegans] ref|NP_740776.1| WW domain Protein (E3 ubiquitin ligase) family member (wwp-1) [Caenorhabditis elegans] 0.0
blast AAN73849 Ww domain protein (e3 ubiquitin ligase) protein 1, isoform a [Caenorhabditis elegans] ref|NP_740775.1| WW domain Protein (E3 ubiquitin ligase) family member (wwp-1) [Caenorhabditis elegans] 0.0
blast CAE61291 Hypothetical protein CBG05115 [Caenorhabditis briggsae] 0.0
blast AAO21415 Hypothetical protein Y65B4BR.5b [Caenorhabditis elegans] ref|NP_871846.1| Y65B4BR.5b [Caenorhabditis elegans] sp|Q86S66|NACA_CAEEL Nascent polypeptide-associated complex alpha subunit (NAC-alpha) (Alpha-NAC) 6.0E-95
blast AAF60854 Hypothetical protein Y65B4BR.5a [Caenorhabditis elegans] ref|NP_490749.1| Y65B4BR.5a [Caenorhabditis elegans] 1.0E-94
Protein Domains
Database ID Description E-Value
Pfam IPR002715 EMBL-EBI Nascent polypeptide-associated complex NAC 2.4E-24
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 2.2E-5
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 2.2E-5
Pfam IPR000449 EMBL-EBI Ubiquitin-associated domain 1.6E-6
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 3.5E-15
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 6.0E-16
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 8.9E-13
Pfam IPR001202 EMBL-EBI WW/Rsp5/WWP domain 2.5E-12
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 2.8E-16
Pfam IPR000569 EMBL-EBI HECT domain (Ubiquitin-protein ligase) 1.1E-102
Pfam IPR000008 EMBL-EBI C2 domain 3.1E-7
Sequence
Target Sequence 
>C. ELEGANS:187093_AT
tgaccggaagcaccgaaactcgccagaaggaagtcaaggagccacaagttgacgtttcgg
atgattccgacaacgaggccgtcgagcaagagctcaccgaggagcaaagacgtgtggccg
aggctgctggacttggagatcacatcgacaagcaggccaagcaaagccgctccgagaaga
aggcccgcaagctcttctccaagctcggactcaagcaagtgactggtgtctcccgtgtct
gcattcgcaagtcgaagaacatcctcttcgtcataaacaagccagacgtgttcaagagcc
caggatctgacacctacatcatcttcggagaagccaagatcgaggatctcacccaacacg
cccagatgtctgctattgagaacttgaagccaactcgtgaggccccacaactcaagactg
tcgaagaggacgagaatgaggatgttgaggaggattccaccggcattgaggagaaggaca
tcgagcttgtcatttcccaagccaacaccacccgcaacaaggccatcagggcgcttaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGACCGGAAGCACCGAAACTCGCCA 550 569 14 Antisense
GGAGCCACAAGTTGACGTTTCGGAT 12 523 51 Antisense
GATTCCGACAACGAGGCCGTCGAGC 429 431 76 Antisense
GCCGTCGAGCAAGAGCTCACCGAGG 149 285 91 Antisense
GCAAAGACGTGTGGCCGAGGCTGCT 620 317 117 Antisense
CAAGCAGGCCAAGCAAAGCCGCTCC 303 185 162 Antisense
CTTCTCCAAGCTCGGACTCAAGCAA 213 221 207 Antisense
CAAGCAAGTGACTGGTGTCTCCCGT 635 185 225 Antisense
CAGACGTGTTCAAGAGCCCAGGATC 323 205 296 Antisense
TGACACCTACATCATCTTCGGAGAA 506 569 321 Antisense
GCAACAAGGCCATCAGGGCGCTTAA 225 319 527 Antisense
Sequence Source Affymetrix Proprietary Database