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[187084_at] glutathione S-transferase

Description Y45G12C.2 /REP_DB=WormBase Gene ID /WP=CE21937 /TR=Q9N4X8 /GB=AAF60580.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 25      6grp_high1_10_10 > 52      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 25      6grp_total > 52      4grp_total > 57      6grp_highest_sub > 03      6grp_cgc > 92      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3816
1 2 3 4 range
A 1693.3
(-2.9)
P 		4753.5
(3)
P 		4638.6
(2.8)
P 		1663.8
(-3)
P 		3090
B 1677.5
(-3)
P 		4071
(0.9)
P 		5385.8
(3)
P 		1818.7
(-2.8)
P 		3708
C 1814.4
(-2.6)
P 		4150.6
(1.7)
P 		4854.8
(3)
P 		1569.4
(-3)
P 		3285
avr 1728.4
(-2.9) 		4325
(1.8) 		4959.7
(3) 		1684
(-3) 		3276

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 7712
1 2 3 4 5 6 range
A 2280.6
(-2.2)
P 		4003.3
(-0.8)
P 		8886.2
(3)
P 		4635.7
(-0.3)
P 		2265.9
(-2.2)
P 		1194.7
(-3)
P 		7692
B 2504.7
(-2.2)
P 		4388.7
(-0.7)
P 		8906.5
(3)
P 		4441.6
(-0.6)
P 		2439.4
(-2.3)
P 		1522.8
(-3)
P 		7384
C 2484.3
(-2.1)
P 		4541.3
(-0.1)
P 		7605
(3)
P 		5340.9
(0.7)
P 		2509.1
(-2.1)
P 		1615.5
(-3)
P 		5990
avr 2423.2
(-2.2) 		4311.1
(-0.6) 		8465.9
(3) 		4806.1
(-0.1) 		2404.8
(-2.2) 		1444.3
(-3) 		7022
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1449
1 2 3 4 range
A 616.5
(-0.7)
P 		1031.8
(1.9)
P 		1212.6
(3)
P 		243.5
(-3)
P 		969
B 534.9
(-2)
P 		1005.5
(1.3)
P 		1248
(3)
P 		387.6
(-3)
P 		860
C 600.6
(-1.6)
P 		1193.3
(0.9)
P 		1692
(3)
P 		264.6
(-3)
P 		1427
avr 584
(-1.4) 		1076.9
(1.3) 		1384.2
(3) 		298.6
(-3) 		1086

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1872
1 2 3 4 5 6 range
A 420.4
(-2.5)
P 		1423.8
(1.1)
P 		1935.2
(3)
P 		1175.7
(0.2)
P 		683.7
(-1.6)
P 		290.9
(-3)
P 		1644
B 871.5
(-1.9)
P 		1460.8
(0.4)
P 		2162.8
(3)
P 		1176.4
(-0.7)
P 		991.1
(-1.4)
P 		567.8
(-3)
P 		1595
C 920.8
(-1.4)
P 		1385.7
(0.6)
P 		1935.6
(3)
P 		1074.1
(-0.7)
P 		811.9
(-1.8)
P 		540.8
(-3)
P 		1395
avr 737.6
(-1.9) 		1423.4
(0.7) 		2011.2
(3) 		1142.1
(-0.4) 		828.9
(-1.6) 		466.5
(-3) 		1545
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187084_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15787
Sequence Type Exemplar sequence
Representative Public ID Y45G12C.2 NCBI
Target Description Y45G12C.2 /REP_DB=WormBase Gene ID /WP=CE21937 /TR=Q9N4X8 /GB=AAF60580.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071300(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071300 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-10) (gst-10) mRNA, complete cds. 11/11 A 
SNAP00000043317 ENSEMBL cdna:SNAP chromosome:CEL140:V:2561534:2564403:1 11/11 None
GENEFINDER00000043328 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2564044:2564403:1 11/11 None
Y45G12C.2 ENSEMBL cdna:known chromosome:CEL140:V:2561521:2564525:1 gene:Y45G12C.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2561533-2564403(+) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-10
Ensembl Y45G12C.2
Entrez Gene 178725 Entrez gene
SwissProt Q9N4X8 EMBL-EBI
WormBase CE21937 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1600415_AT similar to Glutathione S-transferase A3-3 (GST class-alpha) cfa
CANINE:1592963_AT similar to Glutathione S-transferase A3-3 (GST class-alpha) cfa
CANINE:1591191_AT similar to Glutathione S-transferase A3-3 (GST class-alpha) cfa
CANINE_2:CFAAFFX.4239.1.S1_S_AT similar to Glutathione S-transferase A3-3 (GST class-alpha) cfa
CHICKEN:GGA.8359.1.S1_AT glutathione S-transferase A3 gga
HG-U95AV2:1260_S_AT glutathione S-transferase A3 hs
HC-G110:1260_S_AT glutathione S-transferase A3 hs
HG-U133_PLUS_2:222102_AT glutathione S-transferase A3 hs
HG-U133A_2:222102_AT glutathione S-transferase A3 hs
HG-FOCUS:222102_AT glutathione S-transferase A3 hs
HG-U133A:222102_AT glutathione S-transferase A3 hs
HU35KSUBA:M14777_S_AT glutathione S-transferase A3 hs
HUGENEFL:X65727_CDS2_S_AT glutathione S-transferase A3 hs
U133_X3P:HS.102484.0.S1_3P_AT glutathione S-transferase A3 hs
HG-U95B:58023_AT glutathione S-transferase A3 hs
MU11KSUBB:MSA.727.0_S_AT glutathione S-transferase, alpha 3 mm
MG-U74AV2:93015_AT glutathione S-transferase, alpha 3 mm
MOE430A:1423436_AT glutathione S-transferase, alpha 3 mm
MOUSE430A_2:1423436_AT glutathione S-transferase, alpha 3 mm
MOUSE430_2:1423436_AT glutathione S-transferase, alpha 3 mm
MOE430A:1423437_AT glutathione S-transferase, alpha 3 mm
MOUSE430A_2:1423437_AT glutathione S-transferase, alpha 3 mm
MOUSE430_2:1423437_AT glutathione S-transferase, alpha 3 mm
MU19KSUBA:TC23238_F_AT Glutathione S-transferase, alpha 3, mRNA (cDNA clone MGC:13726 IMAGE:4210324) mm
MU19KSUBA:TC23238_I_AT Glutathione S-transferase, alpha 3, mRNA (cDNA clone MGC:13726 IMAGE:4210324) mm
MU19KSUBB:TC28888_AT Glutathione S-transferase, alpha 3, mRNA (cDNA clone MGC:13726 IMAGE:4210324) mm
MU19KSUBB:TC32014_AT Glutathione S-transferase, alpha 3, mRNA (cDNA clone MGC:13726 IMAGE:4210324) mm
RAT230_2:1371089_AT glutathione S-transferase Yc2 subunit rn
RAE230A:1371089_AT glutathione S-transferase Yc2 subunit rn
RT-U34:S82820MRNA_S_AT glutathione S-transferase Yc2 subunit rn
RG-U34A:S82820MRNA_S_AT glutathione S-transferase Yc2 subunit rn
RG-U34A:RC_AA945082_AT glutathione S-transferase Yc2 subunit rn
RG-U34A:S72506_S_AT glutathione S-transferase Yc2 subunit rn
Protein Similarities
Method ID Description E-Value
blast NP_503701 Glutathione S-Transferase family member (gst-10) [Caenorhabditis elegans] gb|AAF60580.1| Glutathione s-transferase protein 10 [Caenorhabditis elegans] sp|Q9N4X8|GSTPA_CAEEL Probable glutathione S-transferase P 10 (GST class-pi) 1.0E-121
blast NP_504894 Glutathione S-Transferase family member (gst-41) [Caenorhabditis elegans] gb|AAK72303.2| Glutathione s-transferase protein 41 [Caenorhabditis elegans] 1.0E-93
blast CAE58192 Hypothetical protein CBG01286 [Caenorhabditis briggsae] 2.0E-86
blast NP_503701 Glutathione S-Transferase family member (gst-10) [Caenorhabditis elegans] gb|AAF60580.1| Glutathione s-transferase protein 10 [Caenorhabditis elegans] sp|Q9N4X8|GSTPA_CAEEL Probable glutathione S-transferase P 10 (GST class-pi) 1.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 2.7E-16
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 1.2E-14
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 3.5E-15
Sequence
Target Sequence 
>C. ELEGANS:187084_AT
gtccgttacatttactacgacgagggaacccgcgaggattgtgtcaacaaaaccatcccg
gaggctctagtgaagttggaagagcttttcaaagctcattccggagacttcatcattgga
aacaagattagttatgcggactatattctcttcgaggagttggacgtctaccacgttttg
gatgcgaatatcctagacaaattcccaactctgaagtcgttctgggaaagaatgtggaag
agaccgaatttgaacgcgtatctcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCCGTTACATTTACTACGACGAGG 688 473 325 Antisense
GGGAACCCGCGAGGATTGTGTCAAC 183 489 348 Antisense
TTGTGTCAACAAAACCATCCCGGAG 20 709 363 Antisense
AAACCATCCCGGAGGCTCTAGTGAA 382 115 374 Antisense
AGCTCATTCCGGAGACTTCATCATT 13 81 417 Antisense
ATGCGGACTATATTCTCTTCGAGGA 284 41 458 Antisense
CTCTTCGAGGAGTTGGACGTCTACC 647 227 472 Antisense
GGACGTCTACCACGTTTTGGATGCG 660 523 486 Antisense
AATATCCTAGACAAATTCCCAACTC 602 173 511 Antisense
CAAATTCCCAACTCTGAAGTCGTTC 227 183 522 Antisense
GACCGAATTTGAACGCGTATCTCGA 4 383 566 Antisense
Sequence Source Affymetrix Proprietary Database