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[187081_s_at] Tubulin

Description C47B2.3 /REP_DB=WormBase Gene ID /WP=CE17563 /CHR=1 /FEA=Sanger Annotation /DEF=Tubulin (HINXTON) protein_id:CAB16856.1
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 31      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 05      6grp_total > 33      4grp_total > 58      6grp_cgc > 80      6grp_high1_sub > 03      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9400
1 2 3 4 range
A 7796.6
(-0.4)
P 		12919.9
(3)
P 		11440.9
(2)
P 		3856.8
(-3)
P 		9063
B 7751.9
(-0.4)
P 		10895.2
(2.2)
P 		11876.2
(3)
P 		4509
(-3)
P 		7367
C 7425.8
(0.1)
P 		11075.1
(3)
P 		10345.3
(2.4)
P 		3520.3
(-3)
P 		7555
avr 7658.1
(-0.1) 		11630.1
(3) 		11220.8
(2.7) 		3962
(-3) 		7668

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4005
1 2 3 4 5 6 range
A 7326.9
(2.9)
P 		7295
(2.8)
P 		7354.6
(3)
P 		6401.8
(-0.9)
P 		5955.1
(-2.8)
P 		5894.4
(-3)
P 		1460
B 7373.5
(1)
P 		6978.1
(0.3)
P 		8383.7
(3)
P 		6247
(-1.2)
P 		5857.5
(-1.9)
P 		5301.6
(-3)
P 		3082
C 7240.3
(0.9)
P 		7099.4
(0.7)
P 		8660.7
(3)
P 		5952.8
(-1.1)
P 		6703.9
(0.1)
P 		4655.9
(-3)
P 		4005
avr 7313.6
(1.3) 		7124.2
(0.9) 		8133
(3) 		6200.5
(-1.1) 		6172.2
(-1.1) 		5284
(-3) 		2849
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3041
1 2 3 4 range
A 2838.7
(2.6)
P 		2804.6
(2.5)
P 		2990.9
(3)
P 		564.6
(-3)
P 		2426
B 2472
(2.1)
P 		2690.9
(2.8)
P 		2752
(3)
P 		960.9
(-3)
P 		1791
C 2458.2
(0.7)
P 		3184.2
(2.2)
P 		3605.7
(3)
P 		593.6
(-3)
P 		3012
avr 2589.6
(1.7) 		2893.2
(2.4) 		3116.2
(3) 		706.4
(-3) 		2410

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1487
1 2 3 4 5 6 range
A 1350.7
(-3)
P 		2594.4
(3)
P 		1601.7
(-1.8)
P 		1623.6
(-1.7)
P 		1797
(-0.8)
P 		1435.1
(-2.6)
P 		1244
B 2565.6
(3)
P 		2322.7
(1.4)
P 		2035.8
(-0.5)
P 		1654.6
(-3)
P 		2379.8
(1.8)
P 		1976.9
(-0.9)
P 		911
C 2683.7
(3)
P 		2166.2
(0.9)
P 		2204.3
(1.1)
P 		1197.1
(-3)
P 		2169.3
(0.9)
P 		1558.6
(-1.5)
P 		1487
avr 2200
(1.9) 		2361.1
(3) 		1947.3
(0.1) 		1491.8
(-3) 		2115.4
(1.3) 		1656.9
(-1.9) 		869
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187081_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1816
Sequence Type Exemplar sequence
Representative Public ID C47B2.3 NCBI
Target Description C47B2.3 /REP_DB=WormBase Gene ID /WP=CE17563 /CHR=1 /FEA=Sanger Annotation /DEF=Tubulin (HINXTON) protein_id:CAB16856.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025879(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025879 NCBI Caenorhabditis elegans C47B2.3 (Tubulin) mRNA, complete cds. 11/11 None
SNAP00000041228 ENSEMBL cdna:SNAP chromosome:CEL140:I:12972934:12974383:-1 11/11 None
GENEFINDER00000041243 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12972934:12974383:-1 11/11 None
C47B2.3.1 ENSEMBL cdna:known chromosome:CEL140:I:12972812:12974391:-1 gene:C47B2.3 11/11 A 
C47B2.3.2 ENSEMBL cdna:known chromosome:CEL140:I:12972812:12974996:-1 gene:C47B2.3 11/11 A 
C47B2.3.4 ENSEMBL cdna:known chromosome:CEL140:I:12972842:12974867:-1 gene:C47B2.3 11/11 None
C47B2.3.3 ENSEMBL cdna:known chromosome:CEL140:I:12972842:12974897:-1 gene:C47B2.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12972864-12974314(-) 100.0 100.0
Public Domain and Genome References
Gene Title Tubulin
Gene Symbol tba-2
Ensembl C47B2.3
Entrez Gene 173167 Entrez gene
SwissProt P34690 EMBL-EBI
WormBase CE17563 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:16458_S_AT tubulin alpha-3/alpha-5 chain (TUA3) at
ATH1-121501:245915_S_AT tubulin alpha-3/alpha-5 chain (TUA3) at
CANINE:1604133_S_AT similar to tubulin, alpha 1 cfa
CANINE:1585260_AT similar to tubulin, alpha 1 cfa
CANINE_2:CFA.1372.3.S1_S_AT similar to tubulin, alpha 1 cfa
CANINE_2:CFAAFFX.13695.1.S1_AT similar to tubulin, alpha 1 cfa
CANINE_2:CFAAFFX.26512.1.S1_AT similar to tubulin, alpha 1 cfa
DROSOPHILA_2:1623910_AT Tubulin at 85E dm
DROSGENOME1:143389_F_AT Tubulin at 85E dm
CHICKEN:GGA.8347.1.S1_AT similar to Hypothetical protein MGC69264 gga
HG-U95AV2:40567_AT tubulin, alpha 3 hs
HG-U133A:209118_S_AT tubulin, alpha 3 hs
HG-U133_PLUS_2:209118_S_AT tubulin, alpha 3 hs
HG-U133A_2:209118_S_AT tubulin, alpha 3 hs
HG-FOCUS:209118_S_AT tubulin, alpha 3 hs
HUGENEFL:X01703_AT tubulin, alpha 3 hs
U133_X3P:G4929133_3P_S_AT tubulin, alpha 3 hs
HG-U95C:59760_F_AT tubulin, alpha 3 hs
MU11KSUBA:M13445_F_AT tubulin, alpha 1 mm
MU11KSUBB:MSA.22720.0_S_AT tubulin, alpha 1 mm
MU11KSUBB:MSA.22043.0_F_AT tubulin, alpha 1 mm
MU11KSUBB:MSA.1257.0_F_AT tubulin, alpha 1 mm
MG-U74AV2:98759_F_AT tubulin, alpha 1 mm
MG-U74AV2:100342_I_AT tubulin, alpha 1 mm
MG-U74AV2:100343_F_AT tubulin, alpha 1 mm
MG-U74BV2:164890_R_AT tubulin, alpha 1 mm
MOE430A:1418884_X_AT tubulin, alpha 1 mm
MOUSE430A_2:1418884_X_AT tubulin, alpha 1 mm
MOUSE430_2:1418884_X_AT tubulin, alpha 1 mm
MU19KSUBA:TC22656_F_AT Alpha-tubulin gene M-alpha-1, 3' end mm
MU19KSUBC:TC35664_AT Alpha-tubulin gene M-alpha-1, 3' end mm
MU19KSUBC:TC35909_F_AT Alpha-tubulin gene M-alpha-1, 3' end mm
MU19KSUBC:TC35910_AT Alpha-tubulin gene M-alpha-1, 3' end mm
RT-U34:V01227_S_AT tubulin, alpha 1 rn
RG-U34A:V01227_S_AT tubulin, alpha 1 rn
RG-U34A:J00797CDS_S_AT tubulin, alpha 1 rn
RG-U34B:RC_AA944275_I_AT tubulin, alpha 1 rn
RAE230A:1367579_A_AT tubulin, alpha 1 rn
RAT230_2:1367579_A_AT tubulin, alpha 1 rn
YEAST_2:1770044_AT Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7018 microtubule-based movement inferred from electronic annotation QuickGO AmiGO
7049 cell cycle inferred from electronic annotation QuickGO AmiGO
46785 microtubule polymerization inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5874 microtubule inferred from electronic annotation QuickGO AmiGO
45298 tubulin inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3924 GTPase activity inferred from electronic annotation QuickGO AmiGO
5198 structural molecule activity inferred from electronic annotation QuickGO AmiGO
5525 GTP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB16856 Hypothetical protein C47B2.3 [Caenorhabditis elegans] ref|NP_001021050.1| C47B2.3 [Caenorhabditis elegans] dbj|BAA03610.1| alpha-2 tubulin [Caenorhabditis elegans] sp|P34690|TBA2_CAEEL Tubulin alpha-2 chain 0.0
blast CAE63472 Hypothetical protein CBG07939 [Caenorhabditis briggsae] 0.0
blast CAB03001 Hypothetical protein F26E4.8 [Caenorhabditis elegans] ref|NP_492600.1| TuBulin, Alpha family member (tba-1) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003008 EMBL-EBI Tubulin/FtsZ, GTPase 2.6E-110
Pfam IPR008280 EMBL-EBI Tubulin/FtsZ, C-terminal 8.7E-79
Sequence
Target Sequence 
>C. ELEGANS:187081_S_AT
tcgatggagccctcaacgttgatctcaacgagttccagaccaacttggtgccatacccaa
gaattcacttcccattggccgcctacactccactcatctctgctgagaaggcctaccacg
aggctctgtccgtcagcgacatcaccaatagctgcttcgagccggctaaccagatggtca
agtgtgatccacgtcacggaaagtacatggctgtgtgcctcttgtacagaggagacgtcg
ttccaaaggacgttaacaccgccatcgctgcaatcaagaccaagagaaccatccaattcg
tcgattggtgcccaacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGATGGAGCCCTCAACGTTGATCT 411 603 737 Antisense
GAGCCCTCAACGTTGATCTCAACGA 649 385 743 Antisense
ACTCCACTCATCTCTGCTGAGAAGG 627 97 823 Antisense
TCTCTGCTGAGAAGGCCTACCACGA 557 607 833 Antisense
CTGAGAAGGCCTACCACGAGGCTCT 126 239 839 Antisense
TCGAGCCGGCTAACCAGATGGTCAA 502 601 893 Antisense
GTCAAGTGTGATCCACGTCACGGAA 382 463 913 Antisense
AGTGTGATCCACGTCACGGAAAGTA 398 59 917 Antisense
CCAAAGGACGTTAACACCGCCATCG 215 275 979 Antisense
CCATCCAATTCGTCGATTGGTGCCC 307 271 1025 Antisense
CCAATTCGTCGATTGGTGCCCAACC 63 269 1029 Antisense
Sequence Source Affymetrix Proprietary Database