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[187071_at]

Description F59B2.5 /REP_DB=WormBase Gene ID /WP=CE00232 /TR=SW:P34481 /GB=CAA77584.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 95      4grp_total > 25      6grp_total_sub > Increase      6grp_rule_based > S5      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 65.5
(-3)
A 		83.8
(-1.7)
A 		125.5
(1.2)
A 		151.3
(3)
M 		86
B 86
(-2.6)
A 		150.3
(3)
A 		81.6
(-3)
A 		85.9
(-2.6)
A 		69
C 45.8
(-3)
A 		98.1
(-0.3)
A 		84.5
(-1)
A 		160.3
(3)
A 		115
avr 65.8
(-3) 		110.7
(1) 		97.2
(-0.2) 		132.5
(3) 		67

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 285
1 2 3 4 5 6 range
A 113.4
(-1.3)
A 		34.2
(-3)
A 		101.4
(-1.5)
A 		202.7
(0.7)
A 		310.5
(3)
P 		266.5
(2)
P 		276
B 78.3
(-2.9)
A 		75.5
(-3)
A 		105.8
(-2.1)
A 		165.5
(-0.4)
P 		281.1
(3)
P 		279.4
(3)
P 		206
C 49.8
(-3)
A 		89
(-2.1)
A 		69.5
(-2.6)
A 		205.4
(0.5)
P 		319.6
(3)
P 		308.8
(2.8)
P 		270
avr 80.5
(-2.6) 		66.2
(-3) 		92.2
(-2.3) 		191.2
(0.2) 		303.7
(3) 		284.9
(2.5) 		238
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 23.9
(-0.7)
A 		18.2
(-3)
A 		32.8
(3)
A 		22.2
(-1.4)
M 		15
B 27.4
(-0.1)
A 		37.1
(3)
A 		18.9
(-2.8)
A 		18.3
(-3)
A 		19
C 15.1
(-3)
A 		28.2
(2.5)
A 		29.5
(3)
A 		27
(2)
A 		14
avr 22.1
(-3) 		27.8
(3) 		27.1
(2.3) 		22.5
(-2.6) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 102
1 2 3 4 5 6 range
A 20.9
(-2.4)
A 		12.1
(-3)
A 		22.1
(-2.3)
A 		51.4
(-0.1)
A 		93.7
(3)
P 		64.9
(0.9)
P 		82
B 27.2
(-2.9)
A 		25.1
(-3)
A 		25.7
(-3)
A 		43.8
(-1.7)
P 		114.2
(3)
P 		104.2
(2.3)
P 		89
C 18.5
(-2.9)
A 		27.2
(-2.3)
A 		17.7
(-3)
A 		41.3
(-1.3)
P 		103.4
(3)
P 		103.4
(3)
P 		86
avr 22.2
(-2.9) 		21.5
(-3) 		21.8
(-3) 		45.5
(-1.3) 		103.8
(3) 		90.8
(2.1) 		82
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187071_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6136
Sequence Type Exemplar sequence
Representative Public ID F59B2.5 NCBI
Target Description F59B2.5 /REP_DB=WormBase Gene ID /WP=CE00232 /TR=SW:P34481 /GB=CAA77584.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066590(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066590 NCBI Caenorhabditis elegans F59B2.5 (F59B2.5) mRNA, complete cds. 11/11 None
SNAP00000012573 ENSEMBL cdna:SNAP chromosome:CEL140:III:8996894:9001693:-1 11/11 None
GENEFINDER00000012585 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9000003:9002470:-1 11/11 None
F59B2.5 ENSEMBL cdna:known chromosome:CEL140:III:8999734:9002470:-1 gene:F59B2.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9000083-9002466(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F59B2.5
Entrez Gene 176273 Entrez gene
SwissProt P34481 EMBL-EBI
WormBase CE34041 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA77584 Hypothetical protein F59B2.5 [Caenorhabditis elegans] ref|NP_498991.2| F59B2.5 [Caenorhabditis elegans] sp|P34481|YMJ5_CAEEL Hypothetical protein F59B2.5 in chromosome III 0.0
blast CAA77585 Hypothetical protein F59B2.3 [Caenorhabditis elegans] ref|NP_498990.1| F59B2.3 [Caenorhabditis elegans] sp|P34480|NAGA_CAEEL Putative N-acetylglucosamine-6-phosphate deacetylase (GlcNAc 6-P deacetylase) 0.0
blast CAE62701 Hypothetical protein CBG06850 [Caenorhabditis briggsae] 1.0E-176
blast CAE62702 Hypothetical protein CBG06851 [Caenorhabditis briggsae] 1.0E-172
blast CAE62702 Hypothetical protein CBG06851 [Caenorhabditis briggsae] 1.0E-164
blast AAW88394 Proteasome regulatory particle, non-atpase-like protein 6, isoform b [Caenorhabditis elegans] ref|NP_001022622.1| proteasome Regulatory Particle, Non-ATPase-like family member (rpn-6) [Caenorhabditis elegans] 5.0E-74
Protein Domains
Database ID Description E-Value
Pfam IPR000717 EMBL-EBI Proteasome component region PCI 2.0E-29
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.5E-5
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.6E-7
Pfam IPR000717 EMBL-EBI Proteasome component region PCI 2.0E-29
Pfam IPR000717 EMBL-EBI Proteasome component region PCI 2.0E-29
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498991.2 1 9-26
F59B2.5 1 9-26
GENEFINDER00000012585 1 9-26
Sequence
Target Sequence 
>C. ELEGANS:187071_AT
acaagcctcagtcgatatgcaatctggcgtattgtattctgccgaggagagagactacaa
aacatcgttctcctatttctacgaagcttttgaaggattcgcaagcattggtgacaagat
taatgcaacttcagcattgaagtacatgattctttgcaagatcatgctcaacgaaactga
gcagttggcaggactgttggctgcaaaggaaatcgtggcctaccagaagagcccacgcat
tattgcgattcgctcaatggctgacgcattccgcaaaagaagtttgaaagatttcgtgaa
agctctggcggagcacaaaatcgagctagtcgaggataaagttgttgctgtgcactcgca
gaatttggagcgtaatatgcttgagaaggaaatctcgcgtgtcattgagccatactcgga
aattgaactttcctacatcgcgagagtcatcggaatgacagttccacccgtcgagagagc
tattgcgagaatgattttagataagaagttgatgggaagcatcgatcaacatggagatac
tgtcgtagtttatccaaaagccgatgcggcaaatcagttcacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAAGCCTCAGTCGATATGCAATCT 466 113 762 Antisense
TCTGGCGTATTGTATTCTGCCGAGG 455 607 784 Antisense
AAAACATCGTTCTCCTATTTCTACG 261 129 820 Antisense
AATCGTGGCCTACCAGAAGAGCCCA 673 167 972 Antisense
GAGCCCACGCATTATTGCGATTCGC 30 387 990 Antisense
GATTCGCTCAATGGCTGACGCATTC 305 433 1008 Antisense
AGTTGTTGCTGTGCACTCGCAGAAT 370 65 1101 Antisense
ATCTCGCGTGTCATTGAGCCATACT 8 33 1153 Antisense
GAACTTTCCTACATCGCGAGAGTCA 328 349 1186 Antisense
CATCGGAATGACAGTTCCACCCGTC 294 211 1209 Antisense
GCCGATGCGGCAAATCAGTTCACAA 201 285 1321 Antisense
Sequence Source Affymetrix Proprietary Database