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[187051_at]

Description F58A4.6 /REP_DB=WormBase Gene ID /WP=CE00222 /TR=SW:P34473 /GB=CAA80163.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 94      4grp_highest_8_8 > 14      4grp_high_8_8 > 15      6grp_highest_nonorm > 73      6grp_total > 84      4grp_total > 16      6grp_highest_sub > In      6grp_high1_sub > In      6grp_total_sub > Hat      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 624
1 2 3 4 range
A 336.8
(-3)
P 		466.7
(-1)
P 		520.7
(-0.2)
P 		729.8
(3)
P 		393
B 389.8
(-2.8)
P 		380.8
(-3)
P 		484.9
(-1.1)
P 		717.3
(3)
P 		337
C 385.3
(-3)
P 		459.2
(-2.2)
P 		477.1
(-2)
P 		961.2
(3)
P 		576
avr 370.6
(-3) 		435.6
(-2.1) 		494.2
(-1.3) 		802.8
(3) 		432

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 567
1 2 3 4 5 6 range
A 323.3
(-3)
P 		431.3
(-1.2)
P 		532.1
(0.4)
P 		524.9
(0.3)
P 		688.9
(3)
P 		665.8
(2.6)
P 		366
B 245
(-3)
P 		475.2
(0)
P 		631.2
(2.1)
P 		540.6
(0.9)
P 		703
(3)
P 		657.9
(2.4)
P 		458
C 314.7
(-3)
P 		437
(-1.5)
P 		508.2
(-0.7)
P 		744.4
(2.2)
P 		670.9
(1.3)
P 		812.3
(3)
P 		498
avr 294.3
(-3) 		447.8
(-0.8) 		557.2
(0.8) 		603.3
(1.4) 		687.6
(2.6) 		712
(3) 		418
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 122.6
(0.7)
P 		101.3
(-3)
P 		136.1
(3)
P 		106.8
(-2.1)
P 		35
B 124.3
(0.1)
P 		94
(-3)
P 		112.4
(-1.1)
P 		152.9
(3)
P 		59
C 127.5
(-3)
P 		132
(-2.3)
P 		166.3
(3)
P 		162.1
(2.4)
P 		39
avr 124.8
(-0) 		109.1
(-3) 		138.3
(2.6) 		140.6
(3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 226
1 2 3 4 5 6 range
A 59.6
(-3)
P 		153.4
(0.8)
P 		115.9
(-0.7)
P 		133.1
(-0)
P 		207.9
(3)
P 		162.1
(1.1)
P 		148
B 85.3
(-3)
P 		158.2
(-0.8)
P 		153.3
(-1)
P 		143.2
(-1.3)
P 		285.6
(3)
P 		245.3
(1.8)
P 		200
C 116.6
(-3)
P 		133.3
(-2.4)
P 		129.3
(-2.5)
P 		149.7
(-1.7)
P 		217.1
(0.9)
P 		271.9
(3)
P 		155
avr 87.2
(-3) 		148.3
(-0.6) 		132.8
(-1.2) 		142
(-0.8) 		236.9
(3) 		226.4
(2.6) 		150
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187051_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6939
Sequence Type Exemplar sequence
Representative Public ID F58A4.6 NCBI
Target Description F58A4.6 /REP_DB=WormBase Gene ID /WP=CE00222 /TR=SW:P34473 /GB=CAA80163.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066726(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066726 NCBI Caenorhabditis elegans F58A4.6 (F58A4.6) mRNA, complete cds. 9/11 None
SNAP00000012194 ENSEMBL cdna:SNAP chromosome:CEL140:III:9614387:9615180:1 9/11 None
GENEFINDER00000012208 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9614387:9615180:1 9/11 None
F58A4.6 ENSEMBL cdna:known chromosome:CEL140:III:9614387:9615024:1 gene:F58A4.6 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9614382-9615020(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F58A4.6
Entrez Gene 186485 Entrez gene
SwissProt P34473 EMBL-EBI
WormBase CE34192 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA80163 Hypothetical protein F58A4.6 [Caenorhabditis elegans] ref|NP_499127.2| F58A4.6 [Caenorhabditis elegans] sp|P34473|YMH6_CAEEL Hypothetical protein F58A4.6 in chromosome III 7.0E-91
blast CAA80163 Hypothetical protein F58A4.6 [Caenorhabditis elegans] ref|NP_499127.2| F58A4.6 [Caenorhabditis elegans] sp|P34473|YMH6_CAEEL Hypothetical protein F58A4.6 in chromosome III 5.0E-89
blast CAE65162 Hypothetical protein CBG10031 [Caenorhabditis briggsae] 2.0E-82
blast CAE65162 Hypothetical protein CBG10031 [Caenorhabditis briggsae] 7.0E-61
blast EAA01449 ENSANGP00000016862 [Anopheles gambiae str. PEST] ref|XP_321802.2| ENSANGP00000016862 [Anopheles gambiae str. PEST] 9.0E-19
Sequence
Target Sequence 
>C. ELEGANS:187051_AT
gaccagtacccaatcactagtattcgaattcacgaatttggacgatttgtacggattttt
gaatattctccagtgtcgtgaagaatattcgtttgctcaaatacgtgccttctataatat
tcccgtcgataagcttcagaaacttcttgtcaacatccaagtgaaaaatccggcacaaaa
tctcgattatgcttgggaaagacgtttgaaacaccatttccgatacatgcttgatctcga
aaagctcatgtggaacttgtctactctaggtggcgcttattcagctatgggagatttcga
tgcaaattatgcgaaagtcgctgcaaaaattactgctcatcaaatcaacttagcgaaaaa
atatggagatccagttattcttgcacgatgctacctgtataccgccctcgcagaagcaca
actcggaaatctctcgcat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACCAGTACCCAATCACTAGTATTC 685 383 48 Antisense
TCGTTTGCTCAAATACGTGCCTTCT 617 603 136 Antisense
ACGTGCCTTCTATAATATTCCCGTC 259 95 150 Antisense
AATATTCCCGTCGATAAGCTTCAGA 108 175 163 Antisense
TCAGAAACTTCTTGTCAACATCCAA 417 621 183 Antisense
GAAACACCATTTCCGATACATGCTT 611 355 255 Antisense
AGCTCATGTGGAACTTGTCTACTCT 624 79 290 Antisense
TCTACTCTAGGTGGCGCTTATTCAG 159 615 307 Antisense
GTCGCTGCAAAAATTACTGCTCATC 133 475 364 Antisense
GTTATTCTTGCACGATGCTACCTGT 643 447 421 Antisense
AGCACAACTCGGAAATCTCTCGCAT 254 77 462 Antisense
Sequence Source Affymetrix Proprietary Database