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[187046_s_at]

Description F59B2.2 /REP_DB=WormBase Gene ID /WP=CE20899 /TR=SW:P34479 /GB=CAA77582.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 99      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 73      6grp_total > 99      4grp_total > 59      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 563
1 2 3 4 range
A 646.2
(1)
P 		672
(1.3)
P 		828.3
(3)
P 		279.1
(-3)
P 		549
B 654.4
(0.5)
P 		747.7
(1.7)
P 		841.9
(3)
P 		395.5
(-3)
P 		446
C 578.4
(-0.2)
P 		755
(2.3)
P 		805.7
(3)
P 		377.1
(-3)
P 		429
avr 626.3
(0.5) 		724.9
(1.7) 		825.3
(3) 		350.6
(-3) 		475

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 489.1
(-0.8)
P 		595.8
(2.3)
P 		413.2
(-3)
P 		611.8
(2.7)
P 		552.8
(1)
P 		621.6
(3)
P 		208
B 489.1
(-0.7)
P 		511
(-0.1)
P 		410.9
(-3)
P 		503.7
(-0.3)
P 		617.1
(3)
P 		525.6
(0.3)
P 		206
C 474.9
(-2.2)
P 		603.5
(0.7)
P 		500.1
(-1.7)
P 		441.7
(-3)
P 		701.6
(3)
P 		590.6
(0.4)
P 		260
avr 484.4
(-1.6) 		570.1
(1.2) 		441.4
(-3) 		519.1
(-0.4) 		623.8
(3) 		579.3
(1.5) 		182
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 240
1 2 3 4 range
A 235.3
(3)
P 		145.9
(0.2)
P 		216.5
(2.4)
P 		40.8
(-3)
P 		195
B 208.7
(3)
P 		184.7
(1.8)
P 		195.1
(2.3)
P 		84.3
(-3)
P 		124
C 191.5
(0.5)
P 		217.1
(1.2)
P 		280.8
(3)
P 		63.6
(-3)
P 		217
avr 211.8
(2.3) 		182.6
(1.3) 		230.8
(3) 		62.9
(-3) 		168

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 162
1 2 3 4 5 6 range
A 90.2
(-3)
P 		211.9
(3)
P 		90
(-3)
P 		155.2
(0.2)
P 		166.8
(0.8)
P 		151.3
(0)
P 		122
B 170.2
(-0.2)
P 		170.1
(-0.2)
P 		99.8
(-3)
P 		133.4
(-1.7)
P 		250.7
(3)
P 		196
(0.8)
P 		151
C 176
(0.8)
P 		184.1
(1.1)
P 		127.3
(-1.3)
P 		88.8
(-3)
P 		227
(3)
P 		197.7
(1.7)
P 		138
avr 145.5
(-0.8) 		188.7
(1.6) 		105.7
(-3) 		125.8
(-1.9) 		214.8
(3) 		181.7
(1.2) 		109
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 187046_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8048
Sequence Type Exemplar sequence
Representative Public ID F59B2.2 NCBI
Target Description F59B2.2 /REP_DB=WormBase Gene ID /WP=CE20899 /TR=SW:P34479 /GB=CAA77582.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066588(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066588 NCBI Caenorhabditis elegans F59B2.2 (F59B2.2) mRNA, complete cds. 11/11 None
SNAP00000012572 ENSEMBL cdna:SNAP chromosome:CEL140:III:8994894:8996686:-1 11/11 None
GENEFINDER00000012590 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8994894:8999672:-1 11/11 None
F59B2.2 ENSEMBL cdna:known chromosome:CEL140:III:8994584:8996692:-1 gene:F59B2.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8994889-8996682(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F59B2.2
Entrez Gene 176271 Entrez gene
SwissProt P34479 EMBL-EBI
WormBase CE20899 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6865 amino acid transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5279 amino acid-polyamine transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA77582 Hypothetical protein F59B2.2 [Caenorhabditis elegans] ref|NP_498989.1| F59B2.2 [Caenorhabditis elegans] sp|P34479|YMJ2_CAEEL Hypothetical protein F59B2.2 in chromosome III 0.0
blast CAE62700 Hypothetical protein CBG06849 [Caenorhabditis briggsae] 0.0
blast CAA77585 Hypothetical protein F59B2.3 [Caenorhabditis elegans] ref|NP_498990.1| F59B2.3 [Caenorhabditis elegans] sp|P34480|NAGA_CAEEL Putative N-acetylglucosamine-6-phosphate deacetylase (GlcNAc 6-P deacetylase) 0.0
blast CAA20995 Hypothetical protein Y38H6C.17 [Caenorhabditis elegans] ref|NP_507960.1| Y38H6C.17 [Caenorhabditis elegans] 2.0E-50
Protein Domains
Database ID Description E-Value
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 1.0E-126
Pfam IPR002422 EMBL-EBI Amino acid/polyamine transporter II 1.0E-126
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.5E-5
Pfam IPR006680 EMBL-EBI Amidohydrolase 1.6E-7
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498989.1 10 67-89,130-152,172-189,196-218,228-247,267-289,319-341,362-379,383-405,426-448
F59B2.2 10 67-89,130-152,172-189,196-218,228-247,267-289,319-341,362-379,383-405,426-448
SNAP00000012572 10 67-89,130-152,172-189,196-218,228-247,267-289,319-341,362-379,383-405,426-448
GENEFINDER00000012590 10 481-503,544-566,586-603,610-632,642-661,681-703,733-755,776-793,797-819,840-862
Sequence
Target Sequence 
>C. ELEGANS:187046_S_AT
ccccattcggagttctgtcgacaactatgatcatttgtactgcattcatgacagctctcg
gatttttcggatacactggatttggagattctattgctccaacaatcacaacgaatgtac
caaaagaaggtctttattcaacagtaaatgtattccttatgcttcaatctcttcttggta
attccattgcaatgtacgtcgtctacgatatgttcttcaatggattccgtcggaaattcg
gtgcccgattcccaaatgttccaaagtggctgtcagataaaggattccgtgtcttctggg
tgcttgtcacgtatcttatggcagttttgattccaaaacttgaaatcatgattccattag
tcggagttacaagtggtgccctgtgtgctttaattttcccaccattcttcgaaatgatca
ccttctggaccgattggaagggacttctgacatatcgtcaacgtatgacaaagatcttca
tcaacctcgtcgtcatggctattggagtatttgccattatcgccggagtctacacaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCCCATTCGGAGTTCTGTCGACAAC 48 261 815 Antisense
GACAGCTCTCGGATTTTTCGGATAC 189 369 864 Antisense
TCCTTATGCTTCAATCTCTTCTTGG 47 593 968 Antisense
AATTCGGTGCCCGATTCCCAAATGT 255 175 1049 Antisense
TAAAGGATTCCGTGTCTTCTGGGTG 347 635 1092 Antisense
GCTTGTCACGTATCTTATGGCAGTT 446 307 1116 Antisense
AGTCGGAGTTACAAGTGGTGCCCTG 402 59 1173 Antisense
GGTGCCCTGTGTGCTTTAATTTTCC 479 501 1189 Antisense
TAATTTTCCCACCATTCTTCGAAAT 509 631 1205 Antisense
TGATCACCTTCTGGACCGATTGGAA 291 563 1229 Antisense
CCATTATCGCCGGAGTCTACACAAA 70 271 1328 Antisense
Sequence Source Affymetrix Proprietary Database