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[187015_at]

Description ZK643.6 /REP_DB=WormBase Gene ID /WP=CE00444 /TR=SW:P30652 /GB=CAA77472.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 94      4grp_total > 02      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 2.7
(-3)
A 		5.7
(-2.5)
A 		9.2
(-2)
A 		40.7
(3)
A 		38
B 6.6
(-2.7)
A 		3.6
(-2.9)
A 		2.7
(-3)
A 		82.1
(3)
P 		79
C 2.8
(-3)
A 		4
(-2.9)
A 		3.3
(-3)
A 		74.7
(3)
A 		72
avr 4
(-3) 		4.4
(-3) 		5.1
(-2.9) 		65.8
(3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 859
1 2 3 4 5 6 range
A 6.3
(-3)
A 		7.3
(-3)
A 		4
(-3)
A 		382.9
(-0)
P 		772.4
(3)
P 		304.1
(-0.7)
P 		768
B 4.8
(-3)
A 		4.5
(-3)
A 		4.1
(-3)
A 		291.4
(-1)
P 		860.9
(3)
P 		318.4
(-0.8)
P 		857
C 2
(-3)
A 		7.7
(-3)
A 		2.5
(-3)
A 		327
(-0.4)
P 		739.5
(3)
P 		403.6
(0.3)
P 		738
avr 4.4
(-3) 		6.5
(-3) 		3.5
(-3) 		333.8
(-0.5) 		790.9
(3) 		342
(-0.4) 		787
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 1
(-3)
A 		1.2
(-2.8)
A 		2.4
(-1.3)
A 		6
(3)
A 		5
B 2.1
(-2.5)
A 		0.9
(-2.9)
A 		0.6
(-3)
A 		17.5
(3)
P 		17
C 0.9
(-3)
A 		1.1
(-2.9)
A 		1.2
(-2.8)
A 		12.6
(3)
A 		12
avr 1.3
(-2.9) 		1.1
(-3) 		1.4
(-2.8) 		12
(3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 349
1 2 3 4 5 6 range
A 1.2
(-3)
A 		2.6
(-3)
A 		0.9
(-3)
A 		97.1
(-0.5)
P 		233.1
(3)
P 		74
(-1.1)
P 		232
B 1.7
(-3)
A 		1.5
(-3)
A 		1
(-3)
A 		77.2
(-1.7)
P 		349.8
(3)
P 		118.7
(-1)
P 		349
C 0.7
(-3)
A 		2.3
(-3)
A 		0.6
(-3)
A 		65.8
(-1.4)
P 		239.3
(3)
P 		135.1
(0.4)
P 		239
avr 1.2
(-3) 		2.1
(-3) 		0.8
(-3) 		80
(-1.3) 		274.1
(3) 		109.3
(-0.6) 		273
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187015_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7038
Sequence Type Exemplar sequence
Representative Public ID ZK643.6 NCBI
Target Description ZK643.6 /REP_DB=WormBase Gene ID /WP=CE00444 /TR=SW:P30652 /GB=CAA77472.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066580(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066580 NCBI Caenorhabditis elegans ZK643.6 (ZK643.6) mRNA, complete cds. 9/11 None
SNAP00000011218 ENSEMBL cdna:SNAP chromosome:CEL140:III:8952864:8958550:-1 9/11 None
GENEFINDER00000011237 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8957659:8960598:-1 10/11 None
ZK643.6 ENSEMBL cdna:known chromosome:CEL140:III:8957659:8958550:-1 gene:ZK643.6 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8957654-8958923(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK643.6
Entrez Gene 191375 Entrez gene
SwissProt P30652 EMBL-EBI
WormBase CE28865 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA77474 Hypothetical protein ZK643.5 [Caenorhabditis elegans] ref|NP_498979.1| ZK643.5 [Caenorhabditis elegans] sp|P30651|YOW5_CAEEL Hypothetical protein ZK643.5 in chromosome III 1.0E-171
blast S23243 hypothetical protein ZK643.5 - Caenorhabditis elegans 1.0E-168
blast CAA77472 Hypothetical protein ZK643.6 [Caenorhabditis elegans] ref|NP_498981.1| ZK643.6 [Caenorhabditis elegans] sp|P30652|YOW6_CAEEL Hypothetical protein ZK643.6 precursor 3.0E-98
blast CAA77472 Hypothetical protein ZK643.6 [Caenorhabditis elegans] ref|NP_498981.1| ZK643.6 [Caenorhabditis elegans] sp|P30652|YOW6_CAEEL Hypothetical protein ZK643.6 precursor 6.0E-89
blast CAA77472 Hypothetical protein ZK643.6 [Caenorhabditis elegans] ref|NP_498981.1| ZK643.6 [Caenorhabditis elegans] sp|P30652|YOW6_CAEEL Hypothetical protein ZK643.6 precursor 5.0E-79
blast CAE62710 Hypothetical protein CBG06864 [Caenorhabditis briggsae] 6.0E-59
blast CAE62710 Hypothetical protein CBG06864 [Caenorhabditis briggsae] 1.0E-50
blast AAF99887 Hypothetical protein C46H11.8 [Caenorhabditis elegans] ref|NP_491508.2| C46H11.8 [Caenorhabditis elegans] 6.0E-17
Protein Domains
Database ID Description E-Value
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 0.033
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.8E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.6E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.6E-4
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 4.9E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.8E-10
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.6E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-11
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 1.4E-11
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000011237 1 4-21
Sequence
Target Sequence 
>C. ELEGANS:187015_AT
gatactatcatcgttacaccgggaaacttcggtttaaaggcacatgactgccttgtgaca
tgcaatgcatgcgaatcctacacttgcaacaacccacagcccgatacaactctcaactgc
acagcacttgctggtgaatgtaactcagcattgtttagtgagcttatgaaggaaaaatgt
ccagcaacgtgtggaaagtgtaatcgaaaaaatgccaatttatgttcggacaagagcaaa
cctgacatttgcgtaaacctaaagacactttgtaattcagtggaattttacgataaactt
tccgaacagtgtccgtcaacttgcaatagatgtccgcataatggaacaaacccggaaaat
aaaactggaggaaatggaggaactggaacacaagaatgtactgatttggcgaatgattgt
tcttacaatcaaaaccgatgttccgtaaaagaatattcttcactgatgcaccgcctatgt
ccaaaaacctgcaacgcttgcaacatctgtgaggatgccaacaaaatgtgcccgatttgg
gtgcctcggggattctgttcgaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATACTATCATCGTTACACCGGGAA 278 423 46 Antisense
GACTGCCTTGTGACATGCAATGCAT 169 375 91 Antisense
GCATGCGAATCCTACACTTGCAACA 508 307 112 Antisense
CAACTCTCAACTGCACAGCACTTGC 471 187 152 Antisense
TAAACTTTCCGAACAGTGTCCGTCA 288 641 339 Antisense
ACAGTGTCCGTCAACTTGCAATAGA 453 107 351 Antisense
AGAATATTCTTCACTGATGCACCGC 42 77 495 Antisense
GCCTATGTCCAAAAACCTGCAACGC 661 279 518 Antisense
GCAACGCTTGCAACATCTGTGAGGA 169 321 536 Antisense
CAAAATGTGCCCGATTTGGGTGCCT 480 189 567 Antisense
GTGCCTCGGGGATTCTGTTCGAAAT 346 477 586 Antisense
Sequence Source Affymetrix Proprietary Database