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[187009_at]

Description Y57G11C.17 /REP_DB=WormBase Gene ID /WP=CE14958 /TR=O18241 /GB=CAB16518.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 40      6grp_high1_10_10 > 05      4grp_high_8_8 > 44      6grp_highest_nonorm > 90      6grp_total > 15      4grp_total > 51      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 158
1 2 3 4 range
A 154.3
(-2.2)
P 		239.6
(3)
P 		140.9
(-3)
P 		173.2
(-1)
P 		99
B 171.9
(3)
P 		171.7
(3)
P 		169
(2.8)
P 		81.9
(-3)
A 		90
C 130.7
(-0.1)
P 		174.6
(3)
P 		151.8
(1.4)
P 		88.7
(-3)
P 		86
avr 152.3
(-0.2) 		195.3
(3) 		153.9
(-0.1) 		114.6
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 137
1 2 3 4 5 6 range
A 238.6
(3)
P 		172.4
(-0.2)
P 		154.8
(-1.1)
P 		115.2
(-3)
P 		149.1
(-1.4)
P 		139.5
(-1.8)
P 		123
B 185.3
(3)
P 		182.7
(2.8)
P 		112.1
(-2.3)
P 		132.2
(-0.8)
P 		124.1
(-1.4)
P 		101.7
(-3)
P 		84
C 189.5
(3)
P 		180.4
(2.3)
P 		166.4
(1.3)
P 		168.8
(1.5)
P 		118.5
(-2.1)
P 		105.7
(-3)
P 		84
avr 204.5
(3) 		178.5
(1.2) 		144.4
(-1.1) 		138.7
(-1.4) 		130.6
(-2) 		115.6
(-3) 		89
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 56.2
(3)
P 		52
(2.2)
P 		36.8
(-0.8)
P 		25.4
(-3)
P 		31
B 54.8
(3)
P 		42.4
(1)
P 		39.2
(0.5)
P 		17.5
(-3)
A 		37
C 43.3
(1.5)
P 		50.2
(2.6)
P 		52.9
(3)
P 		15
(-3)
P 		38
avr 51.4
(3) 		48.2
(2.4) 		43
(1.4) 		19.3
(-3) 		32

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 44
(-0.2)
P 		61.3
(3)
P 		33.7
(-2.2)
P 		29.2
(-3)
P 		45
(-0)
P 		34
(-2.1)
P 		32
B 64.5
(3)
P 		60.8
(2.4)
P 		27.2
(-3)
P 		35
(-1.7)
P 		50.4
(0.7)
P 		37.9
(-1.3)
P 		37
C 70.3
(3)
P 		55.1
(0.5)
P 		42.3
(-1.6)
P 		34
(-3)
P 		38.3
(-2.3)
P 		35.4
(-2.8)
P 		36
avr 59.6
(3) 		59.1
(2.9) 		34.4
(-2.6) 		32.7
(-3) 		44.6
(-0.3) 		35.8
(-2.3) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187009_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8730
Sequence Type Exemplar sequence
Representative Public ID Y57G11C.17 NCBI
Target Description Y57G11C.17 /REP_DB=WormBase Gene ID /WP=CE14958 /TR=O18241 /GB=CAB16518.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070394(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070394 NCBI Caenorhabditis elegans Homolog of Hedgehog AcylTransferase family member (hhat-2) (hhat-2) mRNA, complete cds. 11/11 None
SNAP00000003489 ENSEMBL cdna:SNAP chromosome:CEL140:IV:14826430:14829046:-1 11/11 None
GENEFINDER00000003573 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:14826565:14829046:-1 10/11 None
Y57G11C.17 ENSEMBL cdna:known chromosome:CEL140:IV:14826411:14829077:-1 gene:Y57G11C.17 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:14826435-14828288(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol hhat-2
Ensembl Y57G11C.17
Entrez Gene 190374 Entrez gene
SwissProt O18241 EMBL-EBI
WormBase CE14958 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB16518 Hypothetical protein Y57G11C.17 [Caenorhabditis elegans] ref|NP_502795.1| Homolog of Hedgehog AcylTransferase family member (hhat-2) [Caenorhabditis elegans] 0.0
blast CAE73899 Hypothetical protein CBG21501 [Caenorhabditis briggsae] 0.0
blast CAE73899 Hypothetical protein CBG21501 [Caenorhabditis briggsae] 1.0E-167
blast CAB16518 Hypothetical protein Y57G11C.17 [Caenorhabditis elegans] ref|NP_502795.1| Homolog of Hedgehog AcylTransferase family member (hhat-2) [Caenorhabditis elegans] 1.0E-120
blast CAE73899 Hypothetical protein CBG21501 [Caenorhabditis briggsae] 1.0E-102
blast CAE65899 Hypothetical protein CBG11066 [Caenorhabditis briggsae] 1.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR004299 EMBL-EBI Membrane bound O-acyl transferase, MBOAT 2.7E-46
Pfam IPR004299 EMBL-EBI Membrane bound O-acyl transferase, MBOAT 1.1E-21
Pfam IPR004299 EMBL-EBI Membrane bound O-acyl transferase, MBOAT 3.8E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_502795.1 9 30-52,59-81,85-107,192-214,234-256,308-330,335-357,377-399,423-445
Y57G11C.17 9 30-52,59-81,85-107,192-214,234-256,308-330,335-357,377-399,423-445
SNAP00000003489 5 20-42,62-84,131-153,186-208,232-254
GENEFINDER00000003573 6 20-42,126-143,148-170,281-303,356-378,399-421
Sequence
Target Sequence 
>C. ELEGANS:187009_AT
ttcgcggctctggatggttttcgacctccgccaccacctatctgcatttccagagtgtcg
ctttattccagaatgtggagatttttcgataatggactctatcaattcttaaagtatcag
gtctatatcccagctatgcgaaaaccgttgcctctattttggtccattttgagaagtctt
ggggctctgtgtgcagtttttggtgttgttctagcttggcatggaactcgaaggcacttc
atcttttgggtaacattatcagcaaccgaattgatagtagagcggataggatggcaaatt
tggaaaattcccaaggttcagcgaatcaggaaacgtctcggggaacactgctgcagaaga
attatggcaacactcatgcttctcactgtaacaccaggaattttcggagttttcttcttt
cttggacaggaaggtgttggcgagacgatatctcaaaacgtggtcgtgcaaggagtatta
gatgtattttcaattaatataagtattttcccattaactgccgggtttgcatttctgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCGCGGCTCTGGATGGTTTTCGAC 472 697 781 Antisense
CACCACCTATCTGCATTTCCAGAGT 107 203 812 Antisense
TTTCCAGAGTGTCGCTTTATTCCAG 591 667 827 Antisense
AAGTATCAGGTCTATATCCCAGCTA 467 157 892 Antisense
AGCTATGCGAAAACCGTTGCCTCTA 586 77 912 Antisense
TGAGAAGTCTTGGGGCTCTGTGTGC 262 569 950 Antisense
GTTTTTGGTGTTGTTCTAGCTTGGC 480 449 976 Antisense
GAAGGCACTTCATCTTTTGGGTAAC 165 333 1010 Antisense
TATGGCAACACTCATGCTTCTCACT 409 655 1143 Antisense
GAGTTTTCTTCTTTCTTGGACAGGA 278 399 1187 Antisense
TAACTGCCGGGTTTGCATTTCTGCA 304 637 1295 Antisense
Sequence Source Affymetrix Proprietary Database