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[187003_at]

Description Y116A8C.13 /REP_DB=WormBase Gene ID /WP=CE23323 /TR=Q9U2S8 /GB=CAB55149.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 75      4grp_total > 60      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 77
1 2 3 4 range
A 23
(-3)
A 		46.7
(2.2)
A 		50.4
(3)
A 		48.8
(2.6)
A 		27
B 47.3
(0.9)
A 		8
(-3)
A 		67.8
(3)
A 		11.5
(-2.6)
A 		60
C 75.8
(2.1)
A 		85.1
(3)
A 		30.9
(-2.2)
A 		22.4
(-3)
A 		63
avr 48.7
(2.7) 		46.6
(2.2) 		49.7
(3) 		27.6
(-3) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 112
1 2 3 4 5 6 range
A 8.8
(-3)
A 		26.5
(-1.9)
A 		21.2
(-2.2)
A 		70.4
(0.8)
A 		28.8
(-1.8)
A 		105.7
(3)
A 		97
B 63
(-0.4)
A 		63.2
(-0.3)
A 		65.7
(-0.1)
A 		59.1
(-0.7)
A 		29
(-3)
A 		106
(3)
A 		77
C 43.9
(-1.4)
A 		15.6
(-3)
A 		36.9
(-1.8)
A 		120.9
(3)
A 		41
(-1.6)
A 		106.6
(2.2)
A 		105
avr 38.6
(-2.5) 		35.1
(-2.8) 		41.3
(-2.3) 		83.5
(1.1) 		32.9
(-3) 		106.1
(3) 		73
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 8.4
(-1.7)
A 		10.1
(-0)
A 		13.2
(3)
A 		7.1
(-3)
A 		6
B 15.1
(2.7)
A 		2
(-3)
A 		15.7
(3)
A 		2.5
(-2.8)
A 		14
C 25.1
(3)
A 		24.5
(2.8)
A 		10.8
(-1)
A 		3.8
(-3)
A 		21
avr 16.2
(3) 		12.2
(0.9) 		13.2
(1.5) 		4.5
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 38
1 2 3 4 5 6 range
A 1.6
(-3)
A 		9.4
(-1.1)
A 		4.6
(-2.3)
A 		17.9
(1.1)
A 		8.7
(-1.2)
A 		25.7
(3)
A 		24
B 21.9
(-0.8)
A 		21
(-1)
A 		15.9
(-2.1)
A 		15.7
(-2.2)
A 		11.8
(-3)
A 		39.5
(3)
A 		28
C 16.3
(-0.8)
A 		4.8
(-3)
A 		9.4
(-2.1)
A 		24.3
(0.8)
A 		13.3
(-1.3)
A 		35.7
(3)
A 		31
avr 13.3
(-2.2) 		11.7
(-2.6) 		10
(-3) 		19.3
(-0.6) 		11.3
(-2.7) 		33.6
(3) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 187003_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8447
Sequence Type Exemplar sequence
Representative Public ID Y116A8C.13 NCBI
Target Description Y116A8C.13 /REP_DB=WormBase Gene ID /WP=CE23323 /TR=Q9U2S8 /GB=CAB55149.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070611(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070611 NCBI Caenorhabditis elegans Y116A8C.13 (Y116A8C.13) mRNA, complete cds. 11/11 None
SNAP00000004081 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16956964:16962760:1 11/11 None
GENEFINDER00000004202 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16950065:16962760:1 11/11 None
Y116A8C.13 ENSEMBL cdna:known chromosome:CEL140:IV:16950025:16962760:1 gene:Y116A8C.13 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16950030-16962766(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y116A8C.13
Entrez Gene 190999 Entrez gene
SwissProt Q9U2S8 EMBL-EBI
WormBase CE35647 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB55149 Hypothetical protein Y116A8C.13 [Caenorhabditis elegans] ref|NP_503012.2| Y116A8C.13 [Caenorhabditis elegans] 0.0
blast CAE57388 Hypothetical protein CBG00336 [Caenorhabditis briggsae] 0.0
blast CAE57388 Hypothetical protein CBG00336 [Caenorhabditis briggsae] 1.0E-156
blast EAA04280 ENSANGP00000007696 [Anopheles gambiae str. PEST] ref|XP_308811.2| ENSANGP00000007696 [Anopheles gambiae str. PEST] 1.0E-105
Protein Domains
Database ID Description E-Value
Pfam IPR000330 EMBL-EBI SNF2 related domain 3.4E-62
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 6.4E-24
Pfam IPR000330 EMBL-EBI SNF2 related domain 2.4E-14
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 6.4E-24
Pfam IPR000330 EMBL-EBI SNF2 related domain 3.3E-46
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.2E-17
Sequence
Target Sequence 
>C. ELEGANS:187003_AT
gacaagttcggccatgccacatttatcgattgattaccacgggcacaatcgaggaaaaga
tgctccaacgacaaatcaaaaagaccggtctgggatgtgtgatcgacgcgatcgaggtcg
gcgacacgattgccacgttcaccgacgaggatttgaaggatattttcacttttactggct
cggcgaccgaatgtaatactcatgatctttgcgagtgcccgtgtgatggttgtggaatgc
ttgagaatgagatggacgaggaagacgatggggatgacgtggtggaaaatgatgcggaaa
aaatcgataaatctggaaaaatcgataaaatcgatagcgacgacgatgaggaagccgatg
atgaggagctcgaggcggatgtgaccgatgaagttgtggaggatagtcaggagccccccg
aggccacaaaacccgccgtcgccgccgccggcctccgcacgatcgcctcgctggccgaac
tgtcccgttggcgccacttttcgccacgtcacgccgacacgtggcatcatttcatgacga
atgccgggttgtcgcaggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACAAGTTCGGCCATGCCACATTTA 230 365 1658 Antisense
TTATCGATTGATTACCACGGGCACA 671 677 1680 Antisense
AAGACCGGTCTGGGATGTGTGATCG 68 153 1738 Antisense
TCGACGCGATCGAGGTCGGCGACAC 89 603 1760 Antisense
CTTTTACTGGCTCGGCGACCGAATG 561 219 1826 Antisense
ACTCATGATCTTTGCGAGTGCCCGT 302 99 1855 Antisense
GAGTGCCCGTGTGATGGTTGTGGAA 377 401 1870 Antisense
TGAGGAGCTCGAGGCGGATGTGACC 481 567 2019 Antisense
TGTGGAGGATAGTCAGGAGCCCCCC 683 553 2052 Antisense
ACGCCGACACGTGGCATCATTTCAT 462 91 2168 Antisense
TGACGAATGCCGGGTTGTCGCAGGT 162 569 2192 Antisense
Sequence Source Affymetrix Proprietary Database