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[186999_at]

Description Y45G12C.7 /REP_DB=WormBase Gene ID /WP=CE20501 /TR=Q9UAU5 /GB=AAF60583.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 08      4grp_total > 10      6grp_cgc > 35      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 30
(-3)
A 		47.3
(-1.4)
A 		75.9
(1.2)
A 		95.2
(3)
P 		65
B 51.6
(-0.2)
A 		42.2
(-1.1)
A 		20
(-3)
A 		88.6
(3)
P 		69
C 51.5
(-0.2)
P 		55
(0.1)
A 		19.4
(-3)
A 		88.3
(3)
P 		69
avr 44.4
(-2.3) 		48.2
(-1.9) 		38.4
(-3) 		90.7
(3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 83.1
(2.2)
P 		63.1
(0.2)
P 		85.6
(2.4)
P 		30.5
(-3)
A 		44.6
(-1.6)
A 		91.7
(3)
A 		61
B 96
(3)
P 		56.1
(-1.3)
A 		69.1
(0.1)
P 		78.9
(1.1)
A 		40.9
(-3)
A 		49.1
(-2.1)
P 		55
C 68.1
(0.4)
P 		58.6
(-0.3)
A 		73.4
(0.7)
A 		106.8
(3)
A 		19.1
(-3)
A 		41.4
(-1.5)
P 		88
avr 82.4
(3) 		59.3
(0.1) 		76
(2.2) 		72.1
(1.7) 		34.9
(-3) 		60.7
(0.3) 		48
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 15
1 2 3 4 range
A 10.9
(-2.6)
A 		10.3
(-3)
A 		19.8
(3)
A 		13.9
(-0.7)
P 		10
B 16.4
(2)
A 		10.4
(-0.6)
A 		4.6
(-3)
A 		18.9
(3)
P 		14
C 17
(3)
P 		15.8
(2.3)
A 		6.7
(-3)
A 		14.9
(1.8)
P 		10
avr 14.8
(1.8) 		12.2
(-1) 		10.4
(-3) 		15.9
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 27
1 2 3 4 5 6 range
A 15.3
(0.1)
P 		22.4
(3)
P 		18.7
(1.5)
P 		7.7
(-3)
A 		13.5
(-0.6)
A 		22.3
(3)
A 		15
B 33.4
(3)
P 		18.7
(-2.3)
A 		16.8
(-2.9)
P 		20.9
(-1.5)
A 		16.6
(-3)
A 		18.3
(-2.4)
P 		17
C 25.3
(3)
P 		17.9
(0.7)
A 		18.7
(0.9)
A 		21.5
(1.8)
A 		6.2
(-3)
A 		13.8
(-0.6)
P 		19
avr 24.7
(3) 		19.7
(0.6) 		18.1
(-0.1) 		16.7
(-0.8) 		12.1
(-3) 		18.1
(-0.1) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186999_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15602
Sequence Type Exemplar sequence
Representative Public ID Y45G12C.7 NCBI
Target Description Y45G12C.7 /REP_DB=WormBase Gene ID /WP=CE20501 /TR=Q9UAU5 /GB=AAF60583.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071263(10), NM_001029070(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071263 NCBI Caenorhabditis elegans C13B7.6 (C13B7.6) mRNA, complete cds. 10/11 None
NM_001029070 NCBI Caenorhabditis elegans Y45G12C.16 (Y45G12C.16) mRNA, complete cds. 10/11 None
SNAP00000022915 ENSEMBL cdna:SNAP chromosome:CEL140:V:2421695:2423908:1 10/11 None
GENEFINDER00000022919 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2421695:2422723:1 10/11 None
SNAP00000043308 ENSEMBL cdna:SNAP chromosome:CEL140:V:2529974:2532187:1 10/11 None
GENEFINDER00000043332 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2529974:2531002:1 10/11 None
C13B7.6 ENSEMBL cdna:known chromosome:CEL140:V:2421695:2422723:1 gene:C13B7.6 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2418908-2422723(+) 100.0 100.0
chrV:2527187-2531002(+) 100.0 100.0
Public Domain and Genome References
Gene Title

Ensembl C13B7.6
Entrez Gene 178719 Entrez gene
3565153 Entrez gene
SwissProt Q95X12 EMBL-EBI
Q5CCJ0 EMBL-EBI
WormBase CE27681 Wormbase
CE38242 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001024241 Y45G12C.16 [Caenorhabditis elegans] gb|AAX22281.1| Hypothetical protein Y45G12C.16 [Caenorhabditis elegans] 2.0E-84
blast AAK68175 Hypothetical protein C13B7.6 [Caenorhabditis elegans] ref|NP_503664.1| C13B7.6 [Caenorhabditis elegans] 1.0E-82
blast AAK68175 Hypothetical protein C13B7.6 [Caenorhabditis elegans] ref|NP_503664.1| C13B7.6 [Caenorhabditis elegans] 5.0E-82
blast NP_001024241 Y45G12C.16 [Caenorhabditis elegans] gb|AAX22281.1| Hypothetical protein Y45G12C.16 [Caenorhabditis elegans] 4.0E-81
Sequence
Target Sequence 
>C. ELEGANS:186999_AT
aaaaattccagattcacttcgtcgaaacacgttcaaaaatgccgaaaaatcaagaagatt
ccgaggagaagggcgaaaaagacgagcaaactgcgaaatttcttcacaaactcgcggaaa
acggaaaagatcgcggactgcccgactgtgttgttgctcaatacgagaaaaatgcttggg
aagctacaaacagtgacgaaaagtacaaaagttgtgagaaatgcggaatgccgccaccgc
cgccaattgatggcgcaaagaattctgttgtcgaggaggtatgcaaaaactgcgagacga
tcaacgaaaagcccatcggaattgagtcgcagattctcgaaaagcggcgaaatgaagaga
ttttgaagagaaaagaggagagaaaatctttgaagcgaagtgccgaggaatctgtgacga
attcggcgccagagaaaatccagaaaaaggttcatgtcaaaataatgcctagaaaatgcg
ccccacaaatcctccggcgatcgctcgttccaccgccaatcctcacaccgaagtcggcaa
agaagaagctgaagctcgccgaaaacaaggagcccgatttcttgaaggcg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAATTCCAGATTCACTTCGTCGA 446 129 1007 Antisense
GAAATTTCTTCACAAACTCGCGGAA 166 363 1101 Antisense
CGGAAAAGATCGCGGACTGCCCGAC 152 111 1128 Antisense
CCCGACTGTGTTGTTGCTCAATACG 530 257 1147 Antisense
GAGAAATGCGGAATGCCGCCACCGC 344 393 1222 Antisense
CCACCGCCGCCAATTGATGGCGCAA 177 275 1240 Antisense
AAGCCCATCGGAATTGAGTCGCAGA 559 149 1315 Antisense
GAATCTGTGACGAATTCGGCGCCAG 271 329 1414 Antisense
TAATGCCTAGAAAATGCGCCCCACA 35 637 1469 Antisense
CAATCCTCACACCGAAGTCGGCAAA 660 179 1523 Antisense
CAAGGAGCCCGATTTCTTGAAGGCG 682 181 1572 Antisense
Sequence Source Affymetrix Proprietary Database