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[186992_at]

Description F54C8.6 /REP_DB=WormBase Gene ID /WP=CE00192 /TR=SW:P34444 /GB=CAA80158.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 14      4grp_total > 44      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 51.2
(-0.8)
A 		87.8
(3)
A 		30.1
(-3)
A 		62.9
(0.4)
A 		58
B 52.5
(2.7)
A 		54.7
(3)
A 		41.8
(1)
A 		15.5
(-3)
A 		39
C 73.6
(3)
P 		64.4
(1.2)
M 		43.3
(-3)
M 		46.9
(-2.3)
A 		30
avr 59.1
(1.1) 		69
(3) 		38.4
(-3) 		41.8
(-2.3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 86.9
(3)
P 		48
(-0.8)
P 		52.1
(-0.4)
P 		48.3
(-0.7)
A 		30
(-2.5)
M 		24.8
(-3)
A 		62
B 68.7
(3)
P 		60.7
(1.9)
P 		25.8
(-2.7)
A 		50.6
(0.6)
A 		36.9
(-1.3)
P 		23.9
(-3)
P 		45
C 61.7
(2.7)
P 		61.5
(2.6)
P 		64.2
(3)
P 		64.2
(3)
A 		20.5
(-3)
A 		27.4
(-2.1)
A 		44
avr 72.4
(3) 		56.7
(1) 		47.4
(-0.2) 		54.4
(0.7) 		29.1
(-2.5) 		25.4
(-3) 		47
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 18.6
(2.7)
A 		19.1
(3)
A 		7.9
(-3)
A 		9.2
(-2.3)
A 		11
B 16.7
(3)
A 		13.5
(1.6)
A 		9.7
(-0.1)
A 		3.3
(-3)
A 		13
C 24.4
(3)
P 		18.5
(0.9)
M 		15.1
(-0.4)
M 		7.9
(-3)
A 		17
avr 19.9
(3) 		17
(1.7) 		10.9
(-1.1) 		6.8
(-3) 		13

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 18
1 2 3 4 5 6 range
A 16
(2.4)
P 		17.1
(3)
P 		11.3
(-0.1)
P 		12.2
(0.4)
A 		9
(-1.4)
M 		6
(-3)
A 		11
B 23.9
(3)
P 		20.2
(1.7)
P 		6.3
(-3)
A 		13.4
(-0.6)
A 		15
(-0)
P 		8.9
(-2.1)
P 		18
C 22.9
(3)
P 		18.8
(1.5)
P 		16.3
(0.6)
P 		12.9
(-0.7)
A 		6.6
(-3)
A 		9.2
(-2)
A 		16
avr 20.9
(3) 		18.7
(2) 		11.3
(-1.5) 		12.8
(-0.8) 		10.2
(-2) 		8
(-3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186992_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8065
Sequence Type Exemplar sequence
Representative Public ID F54C8.6 NCBI
Target Description F54C8.6 /REP_DB=WormBase Gene ID /WP=CE00192 /TR=SW:P34444 /GB=CAA80158.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066676(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066676 NCBI Caenorhabditis elegans F54C8.6 (F54C8.6) mRNA, complete cds. 9/11 None
GENEFINDER00000014873 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9441654:9450193:-1 9/11 None
SNAP00000014863 ENSEMBL cdna:SNAP chromosome:CEL140:III:9444305:9446462:-1 9/11 None
F54C8.6 ENSEMBL cdna:known chromosome:CEL140:III:9444305:9446462:-1 gene:F54C8.6 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9444300-9446458(-) 99.89 99.89
Public Domain and Genome References
Gene Title
Ensembl F54C8.6
Entrez Gene 186224 Entrez gene
SwissProt P34444 EMBL-EBI
WormBase CE28832 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA80156 Hypothetical protein F54C8.4 [Caenorhabditis elegans] ref|NP_499076.1| F54C8.4 [Caenorhabditis elegans] sp|P34442|PTP4_CAEEL Probable tyrosine-protein phosphatase F54C8.4 1.0E-173
blast CAA80158 Hypothetical protein F54C8.6 [Caenorhabditis elegans] ref|NP_499077.1| F54C8.6 [Caenorhabditis elegans] sp|P34444|YL86_CAEEL Hypothetical protein F54C8.6 in chromosome III 1.0E-133
blast S40748 hypothetical protein F54C8.6 - Caenorhabditis elegans 1.0E-132
blast CAA80158 Hypothetical protein F54C8.6 [Caenorhabditis elegans] ref|NP_499077.1| F54C8.6 [Caenorhabditis elegans] sp|P34444|YL86_CAEEL Hypothetical protein F54C8.6 in chromosome III 1.0E-132
blast B88555 protein F54C8.6 [imported] - Caenorhabditis elegans 1.0E-127
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499077.1 2 97-119,232-254
F54C8.6 2 97-119,232-254
GENEFINDER00000014873 2 189-211,324-346
SNAP00000014863 2 97-119,232-254
Sequence
Target Sequence 
>C. ELEGANS:186992_AT
gtgtagaggtttcgcctattaccagaaatagtgtagagtcacatttttgtgaatacttcc
cgagtgtccttctgggctttgcaattttcttcttcggaatcacatttatttcctttatca
aacttttgcgtggtgtttctacacaaccatcattcttctgtagatacttctccggaccat
tctcaccattgtttgatgctctttgtgagccggatccatatgttttgtctgaagagttac
caaaagttatgagtggattgcttgatgaaatgtttataacaatctccgaattcttccaaa
caatttcaaaaggtttcactgtgtttggaactttgttcactgcaatttggcatggattgt
ccgaaaatgtcttctttggattccatgagcttggagaaaacctcggagaaaacataaacc
accttctacattttgttgttcaatttttccatcaaattattcatttaattctcagttcaa
ttgcaactattgcaggggccattgccggagttttcacttctgtaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGTAGAGGTTTCGCCTATTACCAG 46 429 374 Antisense
TTGTGAATACTTCCCGAGTGTCCTT 281 707 420 Antisense
GTCCTTCTGGGCTTTGCAATTTTCT 489 477 439 Antisense
GTGTTTCTACACAACCATCATTCTT 382 485 506 Antisense
TTCTGTAGATACTTCTCCGGACCAT 349 693 529 Antisense
GGACCATTCTCACCATTGTTTGATG 520 523 547 Antisense
GAGCCGGATCCATATGTTTTGTCTG 253 385 580 Antisense
GTCCGAAAATGTCTTCTTTGGATTC 59 471 732 Antisense
TAAACCACCTTCTACATTTTGTTGT 341 639 788 Antisense
CAACTATTGCAGGGGCCATTGCCGG 307 189 857 Antisense
TTGCCGGAGTTTTCACTTCTGTACA 366 701 875 Antisense
Sequence Source Affymetrix Proprietary Database