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[186988_at]

Description T09A5.6 /REP_DB=WormBase Gene ID /WP=CE01087 /TR=SW:P45966 /GB=CAA85334.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 57      6grp_high1_10_10 > 85      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 83      6grp_total > 65      4grp_total > 02      6grp_cgc > 57      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 271
1 2 3 4 range
A 245.5
(-2.7)
P 		301.6
(-0.3)
P 		239.5
(-3)
P 		377.8
(3)
P 		138
B 302
(-1.6)
P 		253.6
(-3)
P 		267.7
(-2.6)
P 		466.1
(3)
P 		213
C 286.2
(-2.7)
P 		276.2
(-3)
P 		302.7
(-2.3)
P 		510.1
(3)
P 		234
avr 277.9
(-2.7) 		277.1
(-2.8) 		270
(-3) 		451.3
(3) 		181

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 367
1 2 3 4 5 6 range
A 247.5
(-3)
P 		379
(-0.6)
P 		572.7
(3)
P 		422.3
(0.2)
P 		382.5
(-0.5)
P 		435.9
(0.5)
P 		325
B 400.8
(-3)
P 		501.1
(0.3)
P 		546.2
(1.7)
P 		423.1
(-2.3)
P 		515.8
(0.7)
P 		585.4
(3)
P 		185
C 379.6
(-2.4)
P 		473.1
(-0.3)
P 		354.2
(-3)
P 		419
(-1.5)
P 		445.9
(-0.9)
P 		614.2
(3)
P 		260
avr 342.6
(-3) 		451.1
(0.2) 		491
(1.4) 		421.5
(-0.7) 		448.1
(0.1) 		545.2
(3) 		203
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 89.4
(3)
P 		65.5
(-1.2)
P 		62.6
(-1.7)
P 		55.3
(-3)
P 		34
B 96.3
(2.5)
P 		62.6
(-2.9)
P 		62
(-3)
P 		99.3
(3)
P 		37
C 94.7
(0.5)
P 		79.4
(-3)
P 		105.5
(3)
P 		86
(-1.5)
P 		26
avr 93.5
(3) 		69.2
(-3) 		76.7
(-1.1) 		80.2
(-0.3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 173
1 2 3 4 5 6 range
A 45.6
(-3)
P 		134.8
(3)
P 		124.7
(2.3)
P 		107.1
(1.1)
P 		115.4
(1.7)
P 		106.1
(1.1)
P 		89
B 139.5
(-1.5)
P 		166.8
(0.1)
P 		132.6
(-1.8)
P 		112.1
(-3)
P 		209.6
(2.5)
P 		218.3
(3)
P 		106
C 140.7
(-0.2)
P 		144.4
(-0)
P 		90.2
(-2.7)
P 		84.3
(-3)
P 		144.3
(-0)
P 		205.6
(3)
P 		121
avr 108.6
(-2.4) 		148.7
(0.8) 		115.8
(-1.8) 		101.2
(-3) 		156.4
(1.4) 		176.7
(3) 		76
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186988_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4058
Sequence Type Exemplar sequence
Representative Public ID T09A5.6 NCBI
Target Description T09A5.6 /REP_DB=WormBase Gene ID /WP=CE01087 /TR=SW:P45966 /GB=CAA85334.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063249(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063249 NCBI Caenorhabditis elegans MeDiaTor family member (mdt-10) (mdt-10) mRNA, complete cds. 11/11 None
SNAP00000029436 ENSEMBL cdna:SNAP chromosome:CEL140:II:7853870:7854961:-1 9/11 None
GENEFINDER00000029450 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7853870:7855627:-1 11/11 None
T09A5.6 ENSEMBL cdna:known chromosome:CEL140:II:7853870:7854931:-1 gene:T09A5.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7853866-7854928(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol mdt-10
Ensembl T09A5.6
Entrez Gene 188311 Entrez gene
SwissProt P45966 EMBL-EBI
WormBase CE01087 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1631196_AT Mediator complex subunit 10 dm
DROSGENOME1:148854_AT Mediator complex subunit 10 dm
CHICKEN:GGA.5810.1.S1_AT similar to DNA segment, Chr 13, Wayne State University 50, expressed gga
Protein Similarities
Method ID Description E-Value
blast CAA85334 Hypothetical protein T09A5.6 [Caenorhabditis elegans] ref|NP_495650.1| MeDiaTor family member (mdt-10) [Caenorhabditis elegans] sp|P45966|YNZ6_CAEEL Hypothetical protein T09A5.6 in chromosome III 3.0E-65
blast CAA85334 Hypothetical protein T09A5.6 [Caenorhabditis elegans] ref|NP_495650.1| MeDiaTor family member (mdt-10) [Caenorhabditis elegans] sp|P45966|YNZ6_CAEEL Hypothetical protein T09A5.6 in chromosome III 5.0E-63
blast CAA85334 Hypothetical protein T09A5.6 [Caenorhabditis elegans] ref|NP_495650.1| MeDiaTor family member (mdt-10) [Caenorhabditis elegans] sp|P45966|YNZ6_CAEEL Hypothetical protein T09A5.6 in chromosome III 1.0E-58
blast CAE59836 Hypothetical protein CBG03308 [Caenorhabditis briggsae] 3.0E-53
blast CAE59836 Hypothetical protein CBG03308 [Caenorhabditis briggsae] 6.0E-53
blast CAE59836 Hypothetical protein CBG03308 [Caenorhabditis briggsae] 1.0E-48
blast CAA85335 Hypothetical protein T09A5.7 [Caenorhabditis elegans] ref|NP_495651.1| T09A5.7 [Caenorhabditis elegans] gb|AAG41146.1| 2I146 [Caenorhabditis elegans] sp|P45967|YNZ7_CAEEL Hypothetical UPF0203 protein T09A5.7 in chromosome III 1.0E-29
blast EAA11572 ENSANGP00000021242 [Anopheles gambiae str. PEST] ref|XP_316314.2| ENSANGP00000021242 [Anopheles gambiae str. PEST] 3.0E-22
Protein Domains
Database ID Description E-Value
Pfam IPR007918 EMBL-EBI Protein of unknown function UPF0203 4.8E-36
Sequence
Target Sequence 
>C. ELEGANS:186988_AT
gaaaatgcgagattcatcggagatcttgcttccaattttcaaacaaagtatcaagacgct
ctaaatggccggatatacacactagttagaggtttgcaggatctagatagaatgaaagga
acttttagtgataagaaggttccattggatcttttaccatatcttgatgatggaaaaaat
ccatgtctgtattcgaaacattgtatggagaaaaccttggaaaagaataaagcggtgaat
ggcaagattgagatttacaagaaattccgtgcccatttgatgaaagaattcagtgaagaa
atgcctgatctcgttatgtact

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAATGCGAGATTCATCGGAGATC 429 353 178 Antisense
ATCGGAGATCTTGCTTCCAATTTTC 360 35 193 Antisense
ATCAAGACGCTCTAAATGGCCGGAT 692 25 227 Antisense
AATGGCCGGATATACACACTAGTTA 561 161 241 Antisense
ACACTAGTTAGAGGTTTGCAGGATC 67 107 256 Antisense
GAAGGTTCCATTGGATCTTTTACCA 179 335 312 Antisense
GGATCTTTTACCATATCTTGATGAT 150 511 324 Antisense
AAAAATCCATGTCTGTATTCGAAAC 489 127 352 Antisense
GAGATTTACAAGAAATTCCGTGCCC 171 395 427 Antisense
GAAATTCCGTGCCCATTTGATGAAA 483 363 438 Antisense
GAAATGCCTGATCTCGTTATGTACT 575 359 475 Antisense
Sequence Source Affymetrix Proprietary Database