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[186973_at]

Description R08D7.2 /REP_DB=WormBase Gene ID /WP=CE00290 /TR=SW:P30641 /GB=CAA78048.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 85      4grp_highest_8_8 > 37      4grp_high_8_8 > 02      6grp_highest_nonorm > 97      6grp_total > 75      4grp_total > 07      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 541
1 2 3 4 range
A 814.9
(3)
P 		632.9
(-3)
P 		665
(-1.9)
P 		656.5
(-2.2)
P 		182
B 793.6
(0.4)
P 		634.4
(-3)
P 		640.9
(-2.9)
P 		918
(3)
P 		284
C 761.6
(-0.2)
P 		512.5
(-3)
P 		733.9
(-0.5)
P 		1053.3
(3)
P 		541
avr 790
(1.2) 		593.3
(-3) 		679.9
(-1.2) 		875.9
(3) 		283

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 989
1 2 3 4 5 6 range
A 564.8
(-3)
P 		679.6
(-2.1)
P 		935.1
(0)
P 		992.1
(0.5)
P 		1010.4
(0.6)
P 		1300
(3)
P 		735
B 586.9
(-3)
P 		718.5
(-2.1)
P 		1106.4
(0.7)
P 		1005.2
(-0)
P 		1109.1
(0.7)
P 		1436.2
(3)
P 		849
C 486.1
(-3)
P 		720.5
(-1.6)
P 		828.4
(-0.9)
P 		1099.9
(0.7)
P 		816.4
(-1)
P 		1474.7
(3)
P 		989
avr 545.9
(-3) 		706.2
(-1.9) 		956.6
(-0.1) 		1032.4
(0.4) 		978.6
(0) 		1403.6
(3) 		858
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 201
1 2 3 4 range
A 296.7
(3)
P 		137.4
(-1.8)
P 		173.8
(-0.7)
P 		96.1
(-3)
P 		201
B 253.1
(3)
P 		156.7
(-2.5)
P 		148.5
(-3)
P 		195.6
(-0.3)
P 		105
C 252.1
(2.8)
P 		147.3
(-3)
P 		255.8
(3)
P 		177.6
(-1.3)
P 		109
avr 267.3
(3) 		147.1
(-3) 		192.7
(-0.7) 		156.4
(-2.5) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 432
1 2 3 4 5 6 range
A 104.1
(-3)
P 		241.7
(0.9)
P 		203.6
(-0.2)
P 		251.6
(1.2)
P 		304.9
(2.7)
P 		316.5
(3)
P 		212
B 204.2
(-3)
P 		239.2
(-2.4)
P 		268.7
(-1.8)
P 		266.2
(-1.9)
P 		450.6
(1.5)
P 		535.6
(3)
P 		331
C 180.2
(-3)
P 		219.8
(-2.2)
P 		210.9
(-2.4)
P 		221.2
(-2.2)
P 		264.2
(-1.4)
P 		493.7
(3)
P 		314
avr 162.8
(-3) 		233.6
(-1.5) 		227.7
(-1.6) 		246.3
(-1.2) 		339.9
(0.7) 		448.6
(3) 		286
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186973_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7244
Sequence Type Exemplar sequence
Representative Public ID R08D7.2 NCBI
Target Description R08D7.2 /REP_DB=WormBase Gene ID /WP=CE00290 /TR=SW:P30641 /GB=CAA78048.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066582(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066582 NCBI Caenorhabditis elegans R08D7.2 (R08D7.2) mRNA, complete cds. 11/11 None
SNAP00000003054 ENSEMBL cdna:SNAP chromosome:CEL140:III:8967026:8972253:1 11/11 None
GENEFINDER00000003067 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8967881:8969532:1 11/11 None
R08D7.2 ENSEMBL cdna:known chromosome:CEL140:III:8967872:8969532:1 gene:R08D7.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8967876-8969528(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl R08D7.2
Entrez Gene 176267 Entrez gene
SwissProt P30641 EMBL-EBI
WormBase CE00290 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA78048 Hypothetical protein R08D7.2 [Caenorhabditis elegans] ref|NP_498983.1| R08D7.2 [Caenorhabditis elegans] sp|P30641|YNE2_CAEEL Hypothetical protein R08D7.2 in chromosome III 0.0
blast CAE62693 Hypothetical protein CBG06841 [Caenorhabditis briggsae] 1.0E-164
blast CAE62693 Hypothetical protein CBG06841 [Caenorhabditis briggsae] 1.0E-159
blast CAA78050 Hypothetical protein R08D7.4 [Caenorhabditis elegans] ref|NP_498985.2| R08D7.4 [Caenorhabditis elegans] sp|P30643|YNE4_CAEEL Hypothetical protein R08D7.4 in chromosome III 1.0E-63
blast CAH91373 hypothetical protein [Pongo pygmaeus] 2.0E-9
Protein Domains
Database ID Description E-Value
Pfam IPR007308 EMBL-EBI Protein of unknown function DUF408 5.6E-107
Pfam IPR007308 EMBL-EBI Protein of unknown function DUF408 5.6E-107
Pfam IPR008011 EMBL-EBI Complex 1 LYR protein 2.2E-21
Sequence
Target Sequence 
>C. ELEGANS:186973_AT
aaatcctggttcacacctgaaactcgtaaattggttcgagaaggtgccagaccaaccggt
ggagctgctgagcaaattttgatggattttttgtccggtaaaaaggtggacgcggagaaa
ttggtcaatttgccgaatcttgataagtacaatgtgaaagagaagagattgaatattttc
ctgcattctattaggaatcactggatggatttggaagcacgtttacatttaactccaact
cgtcgtgatattctcagtcgagtagcttcgacattccaactcgattcggaaaatatcact
ggctggacaaagagagaaattaattctatcgttattgctcttttcattgtgatttgcctc
gtcgatgttgagcttggagacgactacttcaagaaggacaacgcttctccagaacttact
gcgatttccaatgaactatgcgggcttgattctttcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAATCCTGGTTCACACCTGAAACTC 582 115 883 Antisense
TCGAGAAGGTGCCAGACCAACCGGT 314 603 918 Antisense
CCAACCGGTGGAGCTGCTGAGCAAA 293 275 934 Antisense
GAAGCACGTTTACATTTAACTCCAA 515 147 1096 Antisense
AACTCCAACTCGTCGTGATATTCTC 384 141 1113 Antisense
GCTTCGACATTCCAACTCGATTCGG 56 305 1147 Antisense
ATTCTATCGTTATTGCTCTTTTCAT 526 11 1205 Antisense
GATTTGCCTCGTCGATGTTGAGCTT 539 427 1233 Antisense
TGTTGAGCTTGGAGACGACTACTTC 698 559 1248 Antisense
ACAACGCTTCTCCAGAACTTACTGC 557 111 1280 Antisense
AACTATGCGGGCTTGATTCTTTCCA 283 137 1316 Antisense
Sequence Source Affymetrix Proprietary Database