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[186972_at]

Description C43C3.3 /REP_DB=WormBase Gene ID /WP=CE23591 /TR=SW:Q09276 /GB=CAA87330.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Cluster 6grp_total > 57      4grp_total > 31      6grp_cgc > 38      6grp_cgc_sub > Da      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 150.8
(-0.1)
A 		117.5
(-3)
A 		125.9
(-2.3)
A 		186.8
(3)
A 		69
B 172.1
(3)
A 		122.3
(0.1)
A 		140.6
(1.1)
A 		70.7
(-3)
A 		101
C 167.9
(2.5)
A 		123.8
(-2.7)
A 		121.7
(-3)
A 		171.8
(3)
A 		50
avr 163.6
(3) 		121.2
(-3) 		129.4
(-1.8) 		143.1
(0.1) 		42

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 145
1 2 3 4 5 6 range
A 128.3
(-1.9)
A 		107.5
(-3)
A 		127.7
(-2)
A 		144.3
(-1.1)
A 		176.4
(0.5)
A 		226.2
(3)
A 		119
B 179.9
(3)
A 		137.6
(-0.7)
M 		162.9
(1.5)
A 		111.6
(-3)
M 		125.6
(-1.8)
A 		172
(2.3)
P 		68
C 140.9
(1)
A 		153.8
(1.9)
A 		125.2
(-0.1)
A 		118.3
(-0.5)
A 		81.6
(-3)
A 		170.9
(3)
P 		89
avr 149.7
(-0.7) 		133
(-2.2) 		138.6
(-1.7) 		124.7
(-3) 		127.9
(-2.7) 		189.7
(3) 		65
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 54.9
(3)
A 		25.5
(-3)
A 		32.9
(-1.5)
A 		27.4
(-2.6)
A 		29
B 54.9
(3)
A 		30.2
(-0.7)
A 		32.6
(-0.4)
A 		15.1
(-3)
A 		40
C 55.6
(3)
A 		35.6
(-1.5)
A 		42.4
(0)
A 		29
(-3)
A 		27
avr 55.1
(3) 		30.4
(-1.7) 		36
(-0.7) 		23.8
(-3) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 23.6
(-3)
A 		38.2
(-0.2)
A 		27.8
(-2.2)
A 		36.6
(-0.5)
A 		53.2
(2.6)
A 		55.1
(3)
A 		32
B 62.6
(2.7)
A 		45.8
(-0.2)
M 		39.6
(-1.2)
A 		29.5
(-3)
M 		51
(0.7)
A 		64.1
(3)
P 		35
C 52.2
(2.1)
A 		46.9
(1.1)
A 		31.9
(-1.5)
A 		23.8
(-3)
A 		26.4
(-2.5)
A 		57.2
(3)
P 		33
avr 46.1
(0.4) 		43.6
(-0.2) 		33.1
(-2.4) 		30
(-3) 		43.5
(-0.2) 		58.8
(3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186972_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16628
Sequence Type Exemplar sequence
Representative Public ID C43C3.3 NCBI
Target Description C43C3.3 /REP_DB=WormBase Gene ID /WP=CE23591 /TR=SW:Q09276 /GB=CAA87330.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077229(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077229 NCBI Caenorhabditis elegans abnormal DYe Filling family member (dyf-8) (dyf-8) mRNA, complete cds. 11/11 None
GENEFINDER00000031850 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9698611:9701107:1 11/11 None
SNAP00000031848 ENSEMBL cdna:SNAP chromosome:CEL140:X:9699868:9701107:1 9/11 None
C43C3.3 ENSEMBL cdna:known chromosome:CEL140:X:9698609:9701609:1 gene:C43C3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9698610-9701107(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dyf-8
Ensembl C43C3.3
Entrez Gene 181183 Entrez gene
SwissProt Q09276 EMBL-EBI
WormBase CE23591 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA87330 Hypothetical protein C43C3.3 [Caenorhabditis elegans] ref|NP_509630.1| abnormal DYe Filling family member (dyf-8) [Caenorhabditis elegans] sp|Q09276|DYF8_CAEEL Protein dyf-8 (Abnormal dye filling protein 8) 0.0
blast B89614 protein C43C3.3 [imported] - Caenorhabditis elegans 0.0
blast CAE69770 Hypothetical protein CBG16051 [Caenorhabditis briggsae] 0.0
blast CAA87330 Hypothetical protein C43C3.3 [Caenorhabditis elegans] ref|NP_509630.1| abnormal DYe Filling family member (dyf-8) [Caenorhabditis elegans] sp|Q09276|DYF8_CAEEL Protein dyf-8 (Abnormal dye filling protein 8) 1.0E-70
Protein Domains
Database ID Description E-Value
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 3.3E-37
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509630.1 1 379-401
C43C3.3 1 379-401
GENEFINDER00000031850 1 379-401
SNAP00000031848 1 107-129
Sequence
Target Sequence 
>C. ELEGANS:186972_AT
gtgaacgaccaaatctacttctccaagttccgtacttttcgcttcggaaatcaaagcacc
gtgtttgttcactgtcaggtacaagtttgtctcaaaaaggacgaatgttcaaagacgtgt
tacaagaaagtcagcgactcaaatctgactgcagaacgtctgcgtttccgtcacaagcgt
agtattactgatttggaacggaggacaacacggtcagcaccaaccgatgataacggttcc
cttgatctcacaaattctctcaccgtcgtaagcagaattgaatctgcagaacttgttgct
tccccgatttcgcaaccaacaattgtcgatacaccaagtgagcaaagaagagacccatgt
ccaaagagctcaaatatgggattcatcccactcatcatcatgggctctcttgcgtctttg
ttactattctccgcaggcgctgcaatttactttgggtgtaaactgaaaagtatgaaaaag
aaggactcctttgacatgatgtctgcattttcaaatccaacagtcagcatgccggtcaca
tattcccattatcaacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGAACGACCAAATCTACTTCTCCA 224 481 757 Antisense
GCACCGTGTTTGTTCACTGTCAGGT 403 313 812 Antisense
CTGCGTTTCCGTCACAAGCGTAGTA 112 213 916 Antisense
ACAACACGGTCAGCACCAACCGATG 615 111 961 Antisense
ATGATAACGGTTCCCTTGATCTCAC 29 47 983 Antisense
CAAATTCTCTCACCGTCGTAAGCAG 427 193 1007 Antisense
ATCCCACTCATCATCATGGGCTCTC 647 37 1141 Antisense
CTTGCGTCTTTGTTACTATTCTCCG 321 219 1165 Antisense
GCAGGCGCTGCAATTTACTTTGGGT 634 309 1189 Antisense
CAAATCCAACAGTCAGCATGCCGGT 84 193 1268 Antisense
CGGTCACATATTCCCATTATCAACG 354 243 1289 Antisense
Sequence Source Affymetrix Proprietary Database