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[186958_s_at]

Description Y47G6A.12 /REP_DB=WormBase Gene ID /WP=CE22098 /TR=Q9N3T9 /GB=AAF60651.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_total > 97      4grp_total > 51      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 173.6
(-0.5)
A 		265.4
(3)
A 		240.6
(2.1)
A 		107.9
(-3)
A 		158
B 195.7
(-0.1)
A 		281
(3)
A 		242.7
(1.6)
A 		113.3
(-3)
A 		168
C 193.8
(-0.6)
A 		284.4
(3)
A 		212.4
(0.1)
A 		133.1
(-3)
A 		151
avr 187.7
(-0.4) 		276.9
(3) 		231.9
(1.3) 		118.1
(-3) 		159

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 596
1 2 3 4 5 6 range
A 179.6
(-2.4)
A 		126.8
(-3)
A 		172.1
(-2.5)
A 		223.8
(-2)
M 		421.6
(0.1)
P 		693.1
(3)
P 		566
B 188.6
(-2.4)
A 		177.4
(-2.5)
A 		149.5
(-3)
A 		303.8
(-0.5)
M 		404.8
(1.2)
P 		517.4
(3)
P 		368
C 137
(-2.5)
A 		97.5
(-3)
A 		176
(-2)
A 		222.9
(-1.3)
A 		407.4
(1.1)
P 		550
(3)
P 		453
avr 168.4
(-2.5) 		133.9
(-3) 		165.9
(-2.6) 		250.2
(-1.5) 		411.3
(0.7) 		586.8
(3) 		453
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 63.2
(3)
A 		57.6
(2.3)
A 		62.9
(3)
A 		15.8
(-3)
A 		47
B 62.4
(2.1)
A 		69.4
(3)
A 		56.2
(1.3)
A 		24.1
(-3)
A 		45
C 64.2
(1.2)
A 		81.8
(3)
A 		74
(2.2)
A 		22.4
(-3)
A 		59
avr 63.3
(2.2) 		69.6
(3) 		64.4
(2.4) 		20.8
(-3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 163
1 2 3 4 5 6 range
A 33.1
(-3)
A 		45.1
(-2.5)
A 		37.5
(-2.8)
A 		56.8
(-2)
M 		127.2
(1.2)
P 		168.8
(3)
P 		136
B 65.6
(-1.9)
A 		59.1
(-2.1)
A 		36.3
(-3)
A 		80.5
(-1.3)
M 		164.5
(1.9)
P 		192.9
(3)
P 		157
C 50.8
(-2.2)
A 		29.8
(-3)
A 		44.8
(-2.4)
A 		44.8
(-2.4)
A 		131.8
(1)
P 		184.1
(3)
P 		154
avr 49.8
(-2.6) 		44.7
(-2.8) 		39.5
(-3) 		60.7
(-2.1) 		141.2
(1.3) 		181.9
(3) 		142
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 186958_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.235
Sequence Type Exemplar sequence
Representative Public ID Y47G6A.12 NCBI
Target Description Y47G6A.12 /REP_DB=WormBase Gene ID /WP=CE22098 /TR=Q9N3T9 /GB=AAF60651.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058759(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058759 NCBI Caenorhabditis elegans SEParase family member (sep-1) (sep-1) mRNA, complete cds. 11/11 None
SNAP00000033969 ENSEMBL cdna:SNAP chromosome:CEL140:I:3433301:3438534:-1 11/11 None
GENEFINDER00000034087 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:3433301:3438534:-1 11/11 None
Y47G6A.12 ENSEMBL cdna:known chromosome:CEL140:I:3433158:3438544:-1 gene:Y47G6A.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:3433300-3438534(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sep-1
Ensembl Y47G6A.12
Entrez Gene 171912 Entrez gene
SwissProt Q9N3T9 EMBL-EBI
WormBase CE22098 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8233 peptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_491160 SEParase family member (sep-1) [Caenorhabditis elegans] gb|AAF60651.1| Separase protein 1 [Caenorhabditis elegans] gb|AAK77200.1| separase [Caenorhabditis elegans] 0.0
blast CAE68974 Hypothetical protein CBG14956 [Caenorhabditis briggsae] 0.0
blast AAA83576 Hypothetical protein ZK430.5 [Caenorhabditis elegans] ref|NP_494778.1| ZK430.5 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005314 EMBL-EBI Peptidase C50, separase 1.0E-126
Pfam IPR005314 EMBL-EBI Peptidase C50, separase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:186958_S_AT
cgattctggactacgcgatggcaaagtgtccactgatcgttggatgcttatggactgtca
ctgatggtgaaatcgatcgtttcttgattcgaatgatcgatgattgtttcgaagattcca
aatcgctcaccggaatcgacaagcttcgtcagctatcggaggctatgcacgaggccagat
caaaagccagactgaaatatttgaccggtgccgctgtggtgatgtatggattgccagttg
ttgcaaagcaaacgacgccattcgtggaaaaggatcagagaaacttgccgcagactccga
agacttcagcccgaacttcgatgcgaatggaaactgtgccgaagacaccgaaacaagaat
ttgttacatcgaagtctgttccaatgactccaattttctcaaataatgagaacaagtctc
caagccgagcaaggatgccaagcagagttctgaagacacctcgtcaggtgaaaacgttcc
aagaggaagatgatgaagctccgaaacggtcgacaacgcggcagctgaaaccgctggttg
caccacctattccagcaacaccgacgactagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGATTCTGGACTACGCGATGGCAAA 488 245 3182 Antisense
AAGTGTCCACTGATCGTTGGATGCT 431 159 3205 Antisense
GTTGGATGCTTATGGACTGTCACTG 160 435 3220 Antisense
TCCAAATCGCTCACCGGAATCGACA 99 587 3298 Antisense
GAATCGACAAGCTTCGTCAGCTATC 381 329 3314 Antisense
GGAGGCTATGCACGAGGCCAGATCA 603 513 3339 Antisense
AATATTTGACCGGTGCCGCTGTGGT 502 173 3377 Antisense
CAGAGAAACTTGCCGCAGACTCCGA 57 207 3457 Antisense
ACTTCAGCCCGAACTTCGATGCGAA 529 103 3484 Antisense
AGCAGAGTTCTGAAGACACCTCGTC 660 77 3622 Antisense
ATTCCAGCAACACCGACGACTAGAA 514 7 3730 Antisense
Sequence Source Affymetrix Proprietary Database