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[186952_at]

Description Y65B4BL.2 /REP_DB=WormBase Gene ID /WP=CE25536 /TR=Q9N303 /GB=AAF60852.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 4grp_highest_8_8 > 12      4grp_high_8_8 > 39      6grp_total > 97      4grp_total > 32      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 271
1 2 3 4 range
A 118.7
(-3)
P 		181.8
(-1.4)
P 		359.7
(3)
P 		221.8
(-0.4)
P 		241
B 150.7
(-3)
P 		184.4
(-2.1)
P 		256.5
(-0.2)
P 		378.5
(3)
P 		228
C 107.9
(-3)
P 		282.4
(1.2)
P 		355.9
(3)
P 		209.1
(-0.6)
P 		248
avr 125.8
(-3) 		216.2
(-0.3) 		324
(3) 		269.8
(1.4) 		198

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1596
1 2 3 4 5 6 range
A 29.3
(-3)
A 		84.7
(-2.8)
A 		140.2
(-2.6)
P 		348.7
(-1.8)
P 		958.4
(0.5)
P 		1625.7
(3)
P 		1596
B 56.1
(-3)
A 		75.6
(-2.9)
A 		156.9
(-2.5)
P 		377.6
(-1.4)
P 		945.7
(1.3)
P 		1297.5
(3)
P 		1241
C 50.3
(-3)
A 		64.9
(-2.9)
P 		191.9
(-2.4)
P 		369.9
(-1.7)
P 		1079.3
(1)
P 		1579.7
(3)
P 		1529
avr 45.2
(-3) 		75.1
(-2.9) 		163
(-2.5) 		365.4
(-1.7) 		994.5
(0.9) 		1501
(3) 		1456
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 92
1 2 3 4 range
A 43.2
(-2)
P 		39.5
(-2.3)
P 		94
(3)
P 		32.5
(-3)
P 		62
B 48
(-2.6)
P 		45.5
(-3)
P 		59.4
(-0.6)
P 		80.7
(3)
P 		35
C 35.7
(-3)
P 		81.2
(0.1)
P 		124
(3)
P 		35.3
(-3)
P 		89
avr 42.3
(-3) 		55.4
(-1.4) 		92.5
(3) 		49.5
(-2.1) 		50

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 523
1 2 3 4 5 6 range
A 5.4
(-3)
A 		30.1
(-2.6)
A 		30.5
(-2.6)
P 		88.4
(-1.7)
P 		289.2
(1.4)
P 		395.8
(3)
P 		390
B 19.5
(-3)
A 		25.2
(-2.9)
A 		38.1
(-2.8)
P 		100
(-2)
P 		384.2
(1.7)
P 		483.8
(3)
P 		464
C 18.7
(-3)
A 		19.8
(-3)
P 		48.8
(-2.6)
P 		74.4
(-2.3)
P 		349.2
(0.9)
P 		528.8
(3)
P 		510
avr 14.5
(-3) 		25
(-2.9) 		39.1
(-2.7) 		87.6
(-2) 		340.9
(1.3) 		469.5
(3) 		455
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186952_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2573
Sequence Type Exemplar sequence
Representative Public ID Y65B4BL.2 NCBI
Target Description Y65B4BL.2 /REP_DB=WormBase Gene ID /WP=CE25536 /TR=Q9N303 /GB=AAF60852.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058341(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058341 NCBI Caenorhabditis elegans Y65B4BL.2 (Y65B4BL.2) mRNA, complete cds. 11/11 None
SNAP00000040877 ENSEMBL cdna:SNAP chromosome:CEL140:I:505682:508879:1 10/11 None
GENEFINDER00000040885 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:506480:508879:1 11/11 None
Y65B4BL.2.2 ENSEMBL cdna:known chromosome:CEL140:I:506480:509070:1 gene:Y65B4BL.2 11/11 None
Y65B4BL.2.1 ENSEMBL cdna:known chromosome:CEL140:I:506480:509398:1 gene:Y65B4BL.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:506479-508879(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y65B4BL.2
Entrez Gene 171642 Entrez gene
SwissProt Q9N303 EMBL-EBI
WormBase CE25536 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF60852 Hypothetical protein Y65B4BL.2 [Caenorhabditis elegans] ref|NP_490742.1| Y65B4BL.2 [Caenorhabditis elegans] 0.0
blast CAE74299 Hypothetical protein CBG22004 [Caenorhabditis briggsae] 1.0E-157
blast CAE74299 Hypothetical protein CBG22004 [Caenorhabditis briggsae] 1.0E-154
Sequence
Target Sequence 
>C. ELEGANS:186952_AT
aatgtctacttcaaaatccccaataacttgaaggaaggaatccccattggcggaggagtt
caattcgttggaaagcgacaagctggagtcgacagtgaaattatgatcaccgaatgttct
ccatgtccagcgttcacttgtaaaaattactcggtctctggtgatacgcgcttgttccaa
gtgtacttgaagccaaattgcgatcatgagcagttggcggagagtgattcgatgggattt
gtggatttcagagagttggagactccatgtagaggaaagttcctggcatgggtccgtgaa
tccattactgtcaatgattgtcgccgtgctgctaccatcatggaagtatgctcgacagcc
atctgtcctccgttgattgcaatgagcgcgaattcgagcagagcaacatcggccagaact
acgccagctggaagttccattggatccagatcatcaattcaatcaagagcatctgcagct
acttcagtgagctcaaaccgattcgttggcccttcaagccgcagaactccatctggta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGTCTACTTCAAAATCCCCAATA 116 163 1303 Antisense
GGAATCCCCATTGGCGGAGGAGTTC 280 531 1339 Antisense
ATGATCACCGAATGTTCTCCATGTC 339 45 1405 Antisense
CCATGTCCAGCGTTCACTTGTAAAA 697 271 1423 Antisense
ATTACTCGGTCTCTGGTGATACGCG 306 13 1448 Antisense
AGGAAAGTTCCTGGCATGGGTCCGT 35 49 1575 Antisense
TGGGTCCGTGAATCCATTACTGTCA 377 553 1591 Antisense
GGAAGTATGCTCGACAGCCATCTGT 444 531 1644 Antisense
GAGCAGAGCAACATCGGCCAGAACT 385 389 1698 Antisense
GAGCATCTGCAGCTACTTCAGTGAG 221 389 1769 Antisense
TCAAGCCGCAGAACTCCATCTGGTA 61 633 1816 Antisense
Sequence Source Affymetrix Proprietary Database