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[186933_at]

Description F54G8.1 /REP_DB=WormBase Gene ID /WP=CE00203 /TR=SW:Q03599 /GB=CAA79559.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 10      4grp_highest_8_8 > 62      4grp_high_8_8 > 27      6grp_total > 08      4grp_total > 37      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 230
1 2 3 4 range
A 195.6
(3)
P 		194.3
(2.9)
P 		82
(-3)
P 		189.1
(2.7)
P 		114
B 218.4
(3)
P 		151
(-0.2)
P 		91.4
(-3)
P 		139.1
(-0.7)
P 		127
C 292.2
(3)
P 		186
(0.2)
P 		62.3
(-3)
P 		233.6
(1.5)
P 		230
avr 235.4
(3) 		177.1
(0.8) 		78.6
(-3) 		187.3
(1.2) 		157

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 249.8
(3)
A 		132
(-2.3)
P 		148.5
(-1.6)
P 		155.5
(-1.2)
P 		116.3
(-3)
P 		128.4
(-2.5)
A 		134
B 185.7
(1.9)
P 		178.7
(1.5)
P 		209.7
(3)
P 		183.7
(1.8)
P 		92.9
(-2.5)
P 		82.2
(-3)
P 		128
C 218.7
(2.9)
P 		180
(0.8)
P 		155.7
(-0.5)
P 		221.3
(3)
P 		117.2
(-2.5)
P 		108
(-3)
P 		113
avr 218.1
(3) 		163.6
(0.1) 		171.3
(0.5) 		186.8
(1.3) 		108.8
(-2.9) 		106.2
(-3) 		112
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 71.2
(3)
P 		42.2
(-0.5)
P 		21.4
(-3)
P 		27.7
(-2.2)
P 		50
B 69.6
(3)
P 		37.3
(-1)
P 		21.2
(-3)
P 		29.6
(-2)
P 		48
C 96.7
(3)
P 		53.5
(-0.5)
P 		21.7
(-3)
P 		39.4
(-1.6)
P 		75
avr 79.2
(3) 		44.3
(-0.6) 		21.4
(-3) 		32.2
(-1.9) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 46.1
(2.7)
A 		46.9
(3)
P 		32.3
(-2.6)
P 		39.4
(0.1)
P 		35.1
(-1.5)
P 		31.3
(-3)
A 		16
B 64.6
(3)
P 		59.5
(2.1)
P 		50.9
(0.6)
P 		48.7
(0.2)
P 		37.8
(-1.7)
P 		30.7
(-3)
P 		34
C 81.1
(3)
P 		54.9
(-0.5)
P 		39.6
(-2.5)
P 		44.5
(-1.9)
P 		37.9
(-2.8)
P 		36.2
(-3)
P 		45
avr 63.9
(3) 		53.8
(1.1) 		40.9
(-1.4) 		44.2
(-0.8) 		36.9
(-2.2) 		32.7
(-3) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 186933_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6304
Sequence Type Exemplar sequence
Representative Public ID F54G8.1 NCBI
Target Description F54G8.1 /REP_DB=WormBase Gene ID /WP=CE00203 /TR=SW:Q03599 /GB=CAA79559.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066628(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066628 NCBI Caenorhabditis elegans F54G8.1 (F54G8.1) mRNA, complete cds. 11/11 None
GENEFINDER00000023866 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:9153819:9157070:1 11/11 None
SNAP00000023860 ENSEMBL cdna:SNAP chromosome:CEL140:III:9155021:9159364:1 10/11 None
F54G8.1 ENSEMBL cdna:known chromosome:CEL140:III:9155021:9157070:1 gene:F54G8.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:9155016-9157066(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54G8.1
Entrez Gene 186261 Entrez gene
SwissProt Q03599 EMBL-EBI
WormBase CE00203 Wormbase
Functional Annotations
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4714 transmembrane receptor protein tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5006 epidermal growth factor receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA79559 Hypothetical protein F54G8.1 [Caenorhabditis elegans] ref|NP_499029.1| F54G8.1 [Caenorhabditis elegans] sp|Q03599|YMB1_CAEEL Hypothetical protein F54G8.1 in chromosome III 0.0
blast CAA79559 Hypothetical protein F54G8.1 [Caenorhabditis elegans] ref|NP_499029.1| F54G8.1 [Caenorhabditis elegans] sp|Q03599|YMB1_CAEEL Hypothetical protein F54G8.1 in chromosome III 1.0E-64
blast AAA96065 Hypothetical protein R03G5.3 [Caenorhabditis elegans] ref|NP_509320.3| R03G5.3 [Caenorhabditis elegans] 3.0E-23
blast AAA96065 Hypothetical protein R03G5.3 [Caenorhabditis elegans] ref|NP_509320.3| R03G5.3 [Caenorhabditis elegans] 2.0E-22
blast CAE72313 Hypothetical protein CBG19450 [Caenorhabditis briggsae] 3.0E-12
blast CAE72313 Hypothetical protein CBG19450 [Caenorhabditis briggsae] 4.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 2.6E-30
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 2.6E-30
Pfam IPR000494 EMBL-EBI Epidermal growth-factor receptor (EGFR), L domain 8.7E-5
Sequence
Target Sequence 
>C. ELEGANS:186933_AT
aacctgatctacctgtaatcttcctccaagaaaatggaaatctctccgaagtagaattct
caaatctcacttcaattccatatttcggaaattatccagatgttctttatgctttctcca
accctctgcttacaaatttatcgttggacacctgcgaaaatctaaactctctaggtccaa
tcacatttgaggaagtatcaaattctatttgtgaaaaccgaacaagctcagttgtgccaa
atcaggacaaaaatcgaactggaaattctgggggtttaatcgttgacgtggaagtttcag
ggaccaattcgactgcgctgagctccgttggaaaagatgggccaccagaaaacaacacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACCTGATCTACCTGTAATCTTCCT 483 147 758 Antisense
ATCTCACTTCAATTCCATATTTCGG 227 33 821 Antisense
AGATGTTCTTTATGCTTTCTCCAAC 606 67 855 Antisense
TCCAACCCTCTGCTTACAAATTTAT 662 587 874 Antisense
GACACCTGCGAAAATCTAAACTCTC 215 367 904 Antisense
AAACTCTCTAGGTCCAATCACATTT 624 127 921 Antisense
CAAGCTCAGTTGTGCCAAATCAGGA 119 187 980 Antisense
TCTGGGGGTTTAATCGTTGACGTGG 293 607 1024 Antisense
TTCAGGGACCAATTCGACTGCGCTG 130 681 1053 Antisense
CTGCGCTGAGCTCCGTTGGAAAAGA 484 237 1070 Antisense
GATGGGCCACCAGAAAACAACACAA 539 399 1093 Antisense
Sequence Source Affymetrix Proprietary Database